Team:Reading/Parts

From 2014.igem.org

(Difference between revisions)
 
(30 intermediate revisions not shown)
Line 1: Line 1:
{{Head}}
{{Head}}
-
 
+
<br><br><br><br><br><br><br><br><br><br>
-
 
+
<html>
<html>
<style type="text/css">
<style type="text/css">
Line 9: Line 8:
<table width="70%" align="center">
<table width="70%" align="center">
-
 
+
<!--Our parts-->
-
 
+
<h3 class="title"> Our parts</h3>
-
</tr>
+
-
 
+
-
 
+
-
</tr>
+
-
+
-
 
+
-
 
+
-
 
+
-
 
+
-
</td>
+
<tr> <td colspan="3"  height="10px"> </td></tr>
<tr> <td colspan="3"  height="10px"> </td></tr>
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
<tr> <td colspan="3"  height="5px"> </td></tr>
<tr> <td colspan="3"  height="5px"> </td></tr>
-
 
<!--Parts Submitted to the Registry  -->
<!--Parts Submitted to the Registry  -->
-
<tr><td > <h3> Parts Submitted to the Registry </h3></td>
+
Genetic modification in
-
<td ></td >
+
Synechocystis
-
<td > <h3>What information do I need to start putting my parts on the Registry? </h3></td>
+
is done by modifying the chromosome,
-
</tr>
+
rather than inserting plasmids. Synechocystis is naturally transformable, and will
-
<tr>
+
undergo homologous recombination between its chromosome and a plasmid
-
<td width="45%"  valign="top">
+
containing a homologous region. This means that plasmids for insertion or
-
<p>
+
deletion need to have regions of ~500 to ~1000bp either side of the inserted
-
An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.
+
sequence, making modifications time consuming or costly depending on your
-
 
+
method of plasmid construction. We will therefore submit all our BioBricks for
-
<p>
+
insertions and deletions to the registry. All of these will contain Kanamycin
-
<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
+
resistance for simple selection of transformants.  
-
</p>
+
<br />
-
 
+
<br />
-
<p>
+
<center><img src="https://static.igem.org/mediawiki/2014/6/6c/Matt_bg11plate_1.jpg" width=600px></center>
-
Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
+
<br />
-
</p>
+
<center>Above: Wild-type <i>Synechocystis</i> transformed with a plasmid carrying kanamycin resistance.</center>
-
 
+
<br />
-
 
+
<br />
-
 
+
The mechanism for each of the BioBricks is the same. They will undergo
-
<h3>When should you put parts into the Registry?</h3>
+
recombination with the region that they share homology with. Knockouts will
-
 
+
undergo recombination with the specified gene, replacing it with kanamycin resistance. Insertions will undergo recombination with a region of the
-
<p>
+
chromosome that is not important for the metabolic
-
As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.  
+
conditions we are using, and will insert the gene of interest along with a kanamycin resistance gene.  
-
</p>
+
<br />
-
</td>
+
<br />
-
 
+
We are creating 4 BioBricks that will be submitted
-
<td > </td>
+
to registry, consisting of 2
-
<td width="45%" valign="top">
+
deletions (<a href="http://parts.igem.org/Part:BBa_K1476003" title="Go to PsaD wiki page">PsaD</a> and <a href="http://parts.igem.org/Part:BBa_K1476000" title="Go to PilT1 wiki page">PilT1</a>) and 2 insertions (<a href="http://parts.igem.org/Part:BBa_K1476004" title="Go to PetF wiki page">PetF</a> and <a href="http://parts.igem.org/Part:BBa_K1476001" title="Go to PilA1 wiki Page">PilA1</a>). Background information on the aim of these parts can be
-
 
+
found in our <a href="https://2014.igem.org/Team:Reading/Project" title="Go to project section">project section</a>. <br />  
-
<p>
+
<br />  
-
The information needed to initially create a part on the Registry is:
+
-
</p>
+
-
<ol>
+
-
 
+
-
<li>Part Name</li>
+
-
<li>Part type</li>
+
-
<li>Creator</li>
+
-
<li>Sequence</li>
+
-
<li>Short Description (60 characters on what the DNA does)</li>
+
-
<li>Long Description (Longer description of what the DNA does)</li>
+
-
<li>Design considerations</li>
+
-
</ol>
+
-
 
+
-
<p>
+
-
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.
+
-
</p>
+
-
 
+
-
<p>
+
-
You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
+
-
</p>
+
-
</td>
+
-
</tr>
+
-
 
+
<tr> <td colspan="3"  height="15px"> </td></tr>
<tr> <td colspan="3"  height="15px"> </td></tr>
-
<tr><td colspan="3" > <h3> Parts Table</h3></td></tr>
+
<tr><td><h3 class="title"> Reading iGEM 2014 parts</h3></td>
-
 
+
<td></td>
-
 
+
-
<tr><td width="45%" colspan="3"  valign="top">
+
-
Any parts your team has created will appear in this table below:</td></tr>
+
</table>
</table>

Latest revision as of 00:31, 18 October 2014

University of Reading
Home Team Project Fuel Cell Parts Human Practices Lab book Protocols Attributions










Our parts

Genetic modification in Synechocystis is done by modifying the chromosome, rather than inserting plasmids. Synechocystis is naturally transformable, and will undergo homologous recombination between its chromosome and a plasmid containing a homologous region. This means that plasmids for insertion or deletion need to have regions of ~500 to ~1000bp either side of the inserted sequence, making modifications time consuming or costly depending on your method of plasmid construction. We will therefore submit all our BioBricks for insertions and deletions to the registry. All of these will contain Kanamycin resistance for simple selection of transformants.


Above: Wild-type Synechocystis transformed with a plasmid carrying kanamycin resistance.


The mechanism for each of the BioBricks is the same. They will undergo recombination with the region that they share homology with. Knockouts will undergo recombination with the specified gene, replacing it with kanamycin resistance. Insertions will undergo recombination with a region of the chromosome that is not important for the metabolic conditions we are using, and will insert the gene of interest along with a kanamycin resistance gene.

We are creating 4 BioBricks that will be submitted to registry, consisting of 2 deletions (PsaD and PilT1) and 2 insertions (PetF and PilA1). Background information on the aim of these parts can be found in our project section.

Reading iGEM 2014 parts

<groupparts>iGEM013 Reading</groupparts>

Facebook
Twitter
rusynbioigem@gmail.com

Retrieved from "http://2014.igem.org/Team:Reading/Parts"