Team:Imperial/Lab-book

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            <h1>Lab-books</h1>
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                        <h2>Introduction</h2>
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                        <p>We kept a record of our lab work across a variety of online documents as well as paper based lab-books. Access our online working documents on this page.</p>
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                        <h2>General Mixed LabWork</h2>
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                        <p> This <a href="https://static.igem.org/mediawiki/2014/8/8c/Mixed_Labbook.pdf">document</a> contains experimental details for some of the following parts of the project
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                          <li>Bulk cellulose growth and processing techniques</li>
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                          <li>Coculture experiments </li>
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                          <li><i>G. xylinus</i> isolation and culturing</li>
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                          <li>Initial cloning work for Acs operon </li>
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                          <li>Preliminary work for isolating curli genes </li>
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                          </ul></p>
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                        <h2>Primer Inventory</h2>
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                        <p>Primers for sequencing, mutagenesis, Gibson Assembly, overlap extension PCR... here is our <a href="https://static.igem.org/mediawiki/2014/d/de/PRIMER-INVENTORY.xls
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                        <h2>Functionalisation cloning</h2>
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                        <p><a href="https://static.igem.org/mediawiki/2014/9/97/Functionalisation_Labwork_record_GC-CM_collated.pdf">This lab-book </a> is a break-away from the mixed lab-book and contains details of cloning the constructs used in the <a href="https://2014.igem.org/Team:Imperial/Functionalisation">functionalisation</a> part of our project.  </p>
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<p> <a href="https://static.igem.org/mediawiki/2014/c/ce/PCR-Digestion-Phos-Ligation-Test_Functionalisation-mainly.xls
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">This spreadsheet</a> also details specific digestions and ligation set-ups for these constructs.
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                        <h2>Interlab study cloning</h2>
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                        <p>Precise cloning reaction set-ups are detailed <a href="https://static.igem.org/mediawiki/2014/a/af/Interlab-study-cloning-lab-work-record.xls">here</a></p>
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                        <h2><i>Gluconacetobacter xylinus</i></h2>
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                        <p><a href="https://static.igem.org/mediawiki/2014/7/71/Michael%27s_Lab_notebook_-_Copy.pdf">Lab-book</a> exclusively for work on <i>G.xylinus</i>, with parts including:
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                        <li> Strain growth and isolation </li>
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                        <li> Parts library and characterisation</li>
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                        <li> Genomic DNA extraction and library preparation </li>
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Latest revision as of 00:30, 18 October 2014

Imperial iGEM 2014

Aqualose

Lab-books

Introduction

We kept a record of our lab work across a variety of online documents as well as paper based lab-books. Access our online working documents on this page.

General Mixed LabWork

This document contains experimental details for some of the following parts of the project

  • Bulk cellulose growth and processing techniques
  • Coculture experiments
  • G. xylinus isolation and culturing
  • Initial cloning work for Acs operon
  • Preliminary work for isolating curli genes

Primer Inventory

Primers for sequencing, mutagenesis, Gibson Assembly, overlap extension PCR... here is our inventory

Functionalisation cloning

This lab-book is a break-away from the mixed lab-book and contains details of cloning the constructs used in the functionalisation part of our project.

This spreadsheet also details specific digestions and ligation set-ups for these constructs.

Interlab study cloning

Precise cloning reaction set-ups are detailed here

Gluconacetobacter xylinus

Lab-book exclusively for work on G.xylinus, with parts including:

  • Strain growth and isolation
  • Parts library and characterisation
  • Genomic DNA extraction and library preparation

Retrieved from "http://2014.igem.org/Team:Imperial/Lab-book"