"
Team:Bielefeld-CeBiTec/Results/rMFC
From 2014.igem.org
| Line 24: | Line 24: | ||
<div id="text"> | <div id="text"> | ||
<p> | <p> | ||
| - | + | Our first module deals with the construction of an <i>E. coli</i> strain, which is able to accept electrons stimulating its metabolism. | |
| - | + | We investigated the effect of the C4 carboxylate transporter DcuB knockout on <i>E. coli</i> KRX. Furthermore we showed the integration of the outer membrane porine OprF (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1172507">BBa_K1172507</a>) into the bacterial genome by replacing the gene of E. coli C4 carboxylate antiporter DcuB. | |
| - | + | The electrobiochemical behavior of <i>E. coli</i> KRX with knocked out C4 carboxylate antiporter DcuB was tested in a <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC/Construction#H-cellResults">H-cell reactor</a>. | |
| + | The funtionality of the outer membrane porin OprF (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1172507">BBa_K1172507</a>) in <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs#KRXdeltadcuB"><i>E.coli</i> KRX ΔdcuB::oprF</a> was investigated with a <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#NPNAssay">NPN-Uptake-Assay</a> (<a href="#Cheng2005">Cheng <i>et al.</i>, 2005</a>). | ||
| + | <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BiologSystem">Biolog® analysis</a> showed that there is no significant respiratory activity of <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs#KRXdeltadcuB"><i>E.coli</i> KRX ΔdcuB::oprF</a> | ||
| + | The results in figure XXX demonstrate that the knockout of C4 carboxylate antiporter <i>dcuB</i> was successful. | ||
| + | Our constructed <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs#KRXdeltadcuB"><i>E. coli</i> KRX ΔdcuB::oprF</a> strain shows no succinate export under anaerobic conditions. This demonstrates a successful knockout of the <i>dcuB</i> gene. | ||
</p> | </p> | ||
</div> | </div> | ||
Revision as of 00:30, 18 October 2014
Module I - Reverse Microbial Fuel Cell (rMFC)
Our first module deals with the construction of an E. coli strain, which is able to accept electrons stimulating its metabolism. We investigated the effect of the C4 carboxylate transporter DcuB knockout on E. coli KRX. Furthermore we showed the integration of the outer membrane porine OprF (BBa_K1172507) into the bacterial genome by replacing the gene of E. coli C4 carboxylate antiporter DcuB. The electrobiochemical behavior of E. coli KRX with knocked out C4 carboxylate antiporter DcuB was tested in a H-cell reactor. The funtionality of the outer membrane porin OprF (BBa_K1172507) in E.coli KRX ΔdcuB::oprF was investigated with a NPN-Uptake-Assay (Cheng et al., 2005). Biolog® analysis showed that there is no significant respiratory activity of E.coli KRX ΔdcuB::oprF The results in figure XXX demonstrate that the knockout of C4 carboxylate antiporter dcuB was successful. Our constructed E. coli KRX ΔdcuB::oprF strain shows no succinate export under anaerobic conditions. This demonstrates a successful knockout of the dcuB gene.
