Team:UT-Dallas/Notebook/9-11
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Made glycerol stock of clones inoculated yesterday. </p> | Made glycerol stock of clones inoculated yesterday. </p> | ||
+ | <p> Test digested clones with SpeI and NheI (all clones are correct!). | ||
+ | <p> Start first batch of double transformations - inoculated competent cells with gRNA/Cas9 and corresponding reporter plasmids: acfA, acfB, ctxA, ctxB. | ||
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Latest revision as of 23:55, 17 October 2014
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Thursday, September 11th, 2014
Made glycerol stock of clones inoculated yesterday. Test digested clones with SpeI and NheI (all clones are correct!). Start first batch of double transformations - inoculated competent cells with gRNA/Cas9 and corresponding reporter plasmids: acfA, acfB, ctxA, ctxB. |