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Team:Linkoping Sweden/Parts
From 2014.igem.org
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<h1>Parts</h1> | <h1>Parts</h1> | ||
| - | <p>We have registered | + | <p>We have registered five different Biobricks in the Official Registry of Parts and in the following section you will find a short description of every Biobrick registered.</p> |
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<p style="font-weight:bold">BBa_K1447002: Epitope 2 + E1010</p> | <p style="font-weight:bold">BBa_K1447002: Epitope 2 + E1010</p> | ||
| - | <p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 2 linked to RFP (E1010).</p | + | <p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 2 linked to RFP (E1010).</p> |
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<div class="image-box" style="width:340px;padding-top:0px;float:left;margin:10px 5px 0px;"> | <div class="image-box" style="width:340px;padding-top:0px;float:left;margin:10px 5px 0px;"> | ||
<a href="#part2" name="modal"> | <a href="#part2" name="modal"> | ||
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<div id="part2" class="window" style="width:800px;height:600px"> | <div id="part2" class="window" style="width:800px;height:600px"> | ||
<img src="https://static.igem.org/mediawiki/2014/3/39/Linkoping_sweden_Parts_IMG_3107%282%29.JPG" width="800px" height="600px" title="Agar-plate"> | <img src="https://static.igem.org/mediawiki/2014/3/39/Linkoping_sweden_Parts_IMG_3107%282%29.JPG" width="800px" height="600px" title="Agar-plate"> | ||
| - | <p>Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Frontside of the plate).</p> | + | <p>Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to Ara h1 epitope 2. (Frontside of the plate).</p> |
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<div id="mask"></div> | <div id="mask"></div> | ||
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| - | <p>Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Frontside of the plate).</p> | + | <p>Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to Ara h1 epitope 2. (Frontside of the plate).</p> |
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<div id="mask"></div> | <div id="mask"></div> | ||
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| - | <p>Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Backside of the plate)</p> | + | <p>Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to Ara h1 epitope 2. (Backside of the plate).</p> |
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</div> | </div> | ||
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| - | <div class="text-panel two-columns" style="padding- | + | <div class="text-panel two-columns" style="padding-top:0px;margin-top:0px;"> |
<div style="display: inline-block;"> | <div style="display: inline-block;"> | ||
| - | <p style="font-weight:bold">BBa_K1447005: | + | <p style="font-weight:bold">BBa_K1447005: Ara h1 Epitopes 1-5 + J06504</p> |
| - | <p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to MCherry (J06504).</p> | + | <p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to MCherry (J06504).</p> |
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<div style="display: inline-block;"> | <div style="display: inline-block;"> | ||
<p style="font-weight:bold">BBa_K1447003: Epitope 2 + J06504</p> | <p style="font-weight:bold">BBa_K1447003: Epitope 2 + J06504</p> | ||
| - | <p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 2 linked to MCherry (J06504).</p> | + | <p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 2 linked to MCherry (J06504).</p> |
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<div class="text-panel" style="margin-top:0px;padding-top:0px"> | <div class="text-panel" style="margin-top:0px;padding-top:0px"> | ||
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<div class="image-box" style="width:340px;padding-top:0px;float:left;margin:10px 5px 0px;"> | <div class="image-box" style="width:340px;padding-top:0px;float:left;margin:10px 5px 0px;"> | ||
<a href="#part5" name="modal"> | <a href="#part5" name="modal"> | ||
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<div id="part5" class="window" style="width:800px;height:600px"> | <div id="part5" class="window" style="width:800px;height:600px"> | ||
<img src="https://static.igem.org/mediawiki/2014/f/ff/Linkoping_sweden_Parts_IMG_3109%282%29.JPG" width="800px" height="600px" title="Agar-plate"> | <img src="https://static.igem.org/mediawiki/2014/f/ff/Linkoping_sweden_Parts_IMG_3109%282%29.JPG" width="800px" height="600px" title="Agar-plate"> | ||
| - | <p>Figure 2A. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2. (Frontside of the plate).</p> | + | <p>Figure 2A. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 2. (Frontside of the plate).</p> |
</div> | </div> | ||
<div id="mask"></div> | <div id="mask"></div> | ||
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| - | <p>Figure 2A. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2. (Frontside of the plate).</p> | + | <p>Figure 2A. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 2. (Frontside of the plate).</p> |
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<div id="part6" class="window" style="width:800px;height:600px"> | <div id="part6" class="window" style="width:800px;height:600px"> | ||
<img src="https://static.igem.org/mediawiki/2014/c/c6/Linkoping_sweden_Parts_IMG_3110%282%29.JPG" width="800px" height="600px" title="Agar-plate"> | <img src="https://static.igem.org/mediawiki/2014/c/c6/Linkoping_sweden_Parts_IMG_3110%282%29.JPG" width="800px" height="600px" title="Agar-plate"> | ||
| - | <p>Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2. (Backside of the plate)</p> | + | <p>Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 2. (Backside of the plate)</p> |
</div> | </div> | ||
<div id="mask"></div> | <div id="mask"></div> | ||
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| - | <p>Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2.(Backside of the plate)</p> | + | <p>Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope Ara h1 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to Ara h1 epitope 2.(Backside of the plate)</p> |
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<p style="font-weight:bold">BBa_K1447004: Epitope 1-5 + E1010</p> | <p style="font-weight:bold">BBa_K1447004: Epitope 1-5 + E1010</p> | ||
| - | <p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to RFP (E1010).</p | + | <p>This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to RFP (E1010).</p> |
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<div class="image-box" style="width:340px;padding-top:0px;float:left;margin:10px 5px 0px;"> | <div class="image-box" style="width:340px;padding-top:0px;float:left;margin:10px 5px 0px;"> | ||
<a href="#part8" name="modal"> | <a href="#part8" name="modal"> | ||
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<div id="part8" class="window" style="width:800px;height:600px"> | <div id="part8" class="window" style="width:800px;height:600px"> | ||
<img src="https://static.igem.org/mediawiki/2014/f/ff/Linkoping_sweden_Parts_IMG_3112%282%29.JPG" width="800px" height="600px" title="Agar-plate"> | <img src="https://static.igem.org/mediawiki/2014/f/ff/Linkoping_sweden_Parts_IMG_3112%282%29.JPG" width="800px" height="600px" title="Agar-plate"> | ||
| - | <p>Figure 3A. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Frontside of the plate).</p> | + | <p>Figure 3A. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to Ara h1 epitope 1-5. (Frontside of the plate).</p> |
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<div id="mask"></div> | <div id="mask"></div> | ||
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| - | <p>Figure 3A. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Frontside of the plate).</p> | + | <p>Figure 3A. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to Ara h1 epitope 1-5. (Frontside of the plate).</p> |
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<div id="part9" class="window" style="width:800px;height:600px"> | <div id="part9" class="window" style="width:800px;height:600px"> | ||
<img src="https://static.igem.org/mediawiki/2014/f/f9/Linkoping_sweden_Parts_IMG_3113%282%29.JPG" width="800px" height="600px" title="Agar-plate"> | <img src="https://static.igem.org/mediawiki/2014/f/f9/Linkoping_sweden_Parts_IMG_3113%282%29.JPG" width="800px" height="600px" title="Agar-plate"> | ||
| - | <p>Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Backside of the plate).</p> | + | <p>Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to Ara h1 epitope 1-5. (Backside of the plate).</p> |
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<div id="mask"></div> | <div id="mask"></div> | ||
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| - | <p>Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Backside of the plate).</p> | + | <p>Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to Ara h1 epitope 1-5. (Backside of the plate).</p> |
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<p>This biobrick contains the sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), as well as a designed His-TEV sequence.</p> | <p>This biobrick contains the sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), as well as a designed His-TEV sequence.</p> | ||
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<a href="#part11" name="modal"> | <a href="#part11" name="modal"> | ||
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<p>Figure 4B. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Frontside of the plate).</p> | <p>Figure 4B. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Frontside of the plate).</p> | ||
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Latest revision as of 23:53, 17 October 2014
Parts
We have registered five different Biobricks in the Official Registry of Parts and in the following section you will find a short description of every Biobrick registered.
<groupparts>iGEM014 Linkoping_Sweden</groupparts>
BBa_K1447002: Epitope 2 + E1010
This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 2 linked to RFP (E1010).
BBa_K1447005: Ara h1 Epitopes 1-5 + J06504
This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to MCherry (J06504).
BBa_K1447003: Epitope 2 + J06504
This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 2 linked to MCherry (J06504).
BBa_K1447004: Epitope 1-5 + E1010
This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of Ara h1 epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to RFP (E1010).
BBa_K1447007: His-TEV
This biobrick contains the sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), as well as a designed His-TEV sequence.
