Team:Groningen:Project:Detection/Pseudonomas

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  Detection of <i>Staphylococcus aureus</i>
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  Quorum sensing pathway in Staphylococcus aureus
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Detection of Pseudomonas aeruginosa
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  The quorum sensing system in <i>Staphylococcus aureus</i> consist of four genes (Figure 1). These genes are controlled by leaky inducible promoter (P2), and encode for two membrane proteins (AgrB and AgrC), one regulator protein (AgrA) and a precursor peptide (AgrD). The membrane protein AgrB cleaves the precursor peptide AgrD so the mature signaling peptide is formed. The mature signal peptide is called autoinducing peptide (AIP). After the AIP is secreted from the cell it is able to bind to the second membrane protein AgrC. After the binding the AgrC phosphorylates AgrA, AgrA is able to induce the P2 promoter which leads to increased production of the AIPs.
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</html>{{:Team:Groningen/Template/MODULE/newfigure|Figure 1|2/27/AGRBDCA_SYSTEM_in_staphylococcus_aureus.art.png|Quorum sensing system of <i>Staphylococcus aureus</i> The precursor peptide, AgrD (1), is modified to AIP by the membrane protein AgrB (2) and translocated over the membrane. The AIP binds to AgrC (3) which in turn phosphorylates AgrA (4) the phosphorylated ArgA induces the P2 promoter.}}<html>
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  How LactoAid detects <i>S. aureus</i>
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Quorum sensing pathway in Pseudomonas aeruginosa
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  In our LactoAid want introduced the DNA encoding for the membrane protein AgrC and the regulator protein ArgA. When <i>Staphylococcus aureus</i> starts to produce the AIPs, the LactoAid should be able to sense the molecules via the same system the <i>S. aureus</i> uses. In figure 1 the designed biobrick for the detection of <i>S. aureus</i> is shown. Unfortunately we were not able to assemble the promoter in this BioBrick. We therefore did not send the part to the registry. After the AgrA is phosphorilated it will induce to the P2 promoter which is part of our secretion system. More about the secretion system can be found at the <a href="https://2014.igem.org/Team:Groningen:Project:Secretion">sectretion page</a> 
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The Quorum sensing system of pseudomonas aeruginosa of multiple hierarchically ordered complex. In one layer of this complex system two proteins are synthesized, LasR and LasI. The LasI produces AHLs which can bind with the LasR. The LasR-AHL couple induces expression of virulence factors LasA protease, alkaline protease and exotoxin A. Moreover, the couple acts as autoinducer on the expression of LasI by influencing the promoter pLasI, increasing the synthesis of AHLs.(Papaioannou, Utari, & Quax, 2013). AHLs can move from cell to cell and thus influencing the QS of other cells in the surrounding.
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</html>{{:Team:Groningen/Template/MODULE/newfigure|Figure 1|b/b2/Groningen_2014_THOMASStpahylococcusaureus4.png|<i>Staphylococcus aureus</i>        picture adopted from center for disease control and prevention}}<html>
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Revision as of 23:50, 17 October 2014

Detection of Pseudomonas aeruginosa
Quorum sensing pathway in Pseudomonas aeruginosa
The Quorum sensing system of pseudomonas aeruginosa of multiple hierarchically ordered complex. In one layer of this complex system two proteins are synthesized, LasR and LasI. The LasI produces AHLs which can bind with the LasR. The LasR-AHL couple induces expression of virulence factors LasA protease, alkaline protease and exotoxin A. Moreover, the couple acts as autoinducer on the expression of LasI by influencing the promoter pLasI, increasing the synthesis of AHLs.(Papaioannou, Utari, & Quax, 2013). AHLs can move from cell to cell and thus influencing the QS of other cells in the surrounding.
Figure 1
 
Figure 1: Vivamus a augue diam. Nam hendrerit nisi ut tellus hendrerit, ac volutpat massa tristique.
 
 
Figure 1
 
Figure 1: Vivamus a augue diam. Nam hendrerit nisi ut tellus hendrerit, ac volutpat massa tristique.
 
 

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