Team:Brasil-SP/Notebook/Protocols

From 2014.igem.org

(Difference between revisions)
 
(20 intermediate revisions not shown)
Line 1: Line 1:
-
<b>The content of this page is merely provisional!</b>
+
{{:Team:Brasil-SP/Templates/Header|headersrc=HeaderNotebookBRASILAP.png}}
-
<p>Well... the page title describe the page by itself!</p>
+
-
 
+
-
{{CSS/Main}}
+
<html>
<html>
-
<!--main content -->
+
<h1 align=center>Experimental Methodology</h1>
-
<table width="70%" align="center">
+
-
<!--navigation menu -->
+
<p>For the assemblies constructuion we addopted the following method:</p>
-
<td align="center" colspan="3">
+
-
<table  width="100%">
+
<div align=center><img src="https://static.igem.org/mediawiki/2014/f/fc/Metodologia_Brasil-SP.png" width="900" height="400"/></div>
-
<tr heigth="15px"></tr>
+
-
<tr heigth="75px">  
+
 +
<br>
 +
<p>We used the pSB1C3 vector from the Biobrick <a href="http://parts.igem.org/Part:BBa_J04450">BBa_J04450</a>. Using this biobrick we added a red/white selection, where the red colonies are those which do not have the assembly, and the white ones are the correct ones.</p>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<div align=center><img src="https://static.igem.org/mediawiki/2014/2/25/Red_and_WHite_plate_.jpg" width="700" height="265"/></div>
-
<a href="https://2014.igem.org/Team:Brasil-SP"style="color:#000000">Home </a> </td>
+
<br>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://2014.igem.org/Team:Brasil-SP/Team"style="color:#000000"> Team </a> </td>
 
-
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=Brasil-SP"style="color:#000000"> Official Team Profile </a></td>
 
-
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
 
-
<a href="https://2014.igem.org/Team:Brasil-SP/Project"style="color:#000000"> Project</a></td>
 
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://2014.igem.org/Team:Brasil-SP/Parts"style="color:#000000"> Parts</a></td>
 
-
 
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://2014.igem.org/Team:Brasil-SP/Modeling"style="color:#000000"> Modeling</a></td>
 
-
 
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
 
-
<a href="https://2014.igem.org/Team:Brasil-SP/Notebook"style="color:#000000"> Notebook</a></td>
 
-
 
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://2014.igem.org/Team:Brasil-SP/Safety"style=" color:#000000"> Safety </a></td>
 
-
 
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://2014.igem.org/Team:Brasil-SP/Attributions"style="color:#000000"> Attributions </a></td>
 
-
 
-
 
-
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
 
-
</tr>
 
-
</table>
 
-
 
-
<!--end navigation menu -->
 
Line 91: Line 59:
     <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf">PCR</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf">PCR</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf">Agarose Gel Electrophoresis and DNA Gel Purification</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf">Agarose Gel Electrophoresis and DNA Gel Purification</a></li>
-
     <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Cloning.pdf">PCR Product cloning</a></li>
+
     <li><a href="https://static.igem.org/mediawiki/2014/2/23/PCR_Product_Clonig_2.pdf">PCR Product cloning</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf">Competent <i>E. coli</i> Cells using Calcium Chloride</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf">Competent <i>E. coli</i> Cells using Calcium Chloride</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf">Transformation in <i>E. coli</i> DH5-alpha</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf">Transformation in <i>E. coli</i> DH5-alpha</a></li>
Line 97: Line 65:
     <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf">Cohesive-end assembly cloning</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf">Cohesive-end assembly cloning</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf">Restriction Analysis and Digestion in large scale</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf">Restriction Analysis and Digestion in large scale</a></li>
-
     <li><a href="https://static.igem.org/mediawiki/2014/8/8e/Bacillus_competent_cells.pdf">Competent <i>Bacillus subtilis</i> cells</a></li>
+
     <li><a href="https://static.igem.org/mediawiki/2014/6/66/Bacillus_Competent_cells.pdf">Competent <i>Bacillus subtilis</i> cells</a></li>
-
     <li><a href="https://static.igem.org/mediawiki/2014/8/84/Transformation_Bacilus.pdf">Transformation of <i>Bacillus subtilis</i></a></li>
+
     <li><a href="https://static.igem.org/mediawiki/2014/a/a6/Transformation.pdf">Transformation of <i>Bacillus subtilis</i></a></li>
-
     <li><a href="https://static.igem.org/mediawiki/2014/3/34/DSM_Medium_Sporulation.pdf">DSM Medium for Sporulation</a></li>
+
     <li><a href="https://static.igem.org/mediawiki/2014/3/34/DSM_Medium_Sporulation.pdf">DSM medium for sporulation</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/0/00/Medium_LB.pdf">Luria-Bertani (LB) medium</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/0/00/Medium_LB.pdf">Luria-Bertani (LB) medium</a></li>
-
     <li><a href="https://static.igem.org/mediawiki/2014/0/07/Neubauer_chamber.pdf">Cell counting with Neubauer Chamber</a></li>
+
     <li><a href="https://static.igem.org/mediawiki/2014/0/0e/Neubauer_chamber_2.pdf">Cell counting with Neubauer Chamber</a></li>
 +
    <li><a href="https://static.igem.org/mediawiki/2014/a/a2/Germination_Sporulation.pdf">Germination of <i>Bacillus subtilis</i> spores</a></li>
 +
    <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf">QuickChange PCR</a></li>
 +
    <li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf">PCR for Synthesis</a></li>
   </ul>
   </ul>
Line 110: Line 81:
<!--end lab protocols-->
<!--end lab protocols-->
 +
<br>
Line 124: Line 96:
<h2 align="left"><b>Characterization Protocols</b></h2>
<h2 align="left"><b>Characterization Protocols</b></h2>
 +
    <li><a href="https://static.igem.org/mediawiki/2014/0/00/Cytometry_protocol_characterization.pdf">Cytometry Protocol</a></li>
 +
    <li><a href="https://static.igem.org/mediawiki/2014/0/02/Fluorometry_Protocol_Characterization.pdf">Fluorometry Protocol</a></li>
 +
<br><br><br>
-
<!--track measurement-->
 
 +
</ul> 
-
<p><h2>Measurement Interlab Study</h2></p>
 
-
<p> As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are<p/>
 
-
<tr>
 
-
 
-
<td colspan="2" align="center">
 
-
 
-
<p align="center"> Biobrick device 1: BBa_I20260, an existing device </p>
 
-
 
-
<br>
 
-
 
-
<a href="https://static.igem.org/mediawiki/2014/c/c1/Assembly_Biobrick_Device_1_Measurement_Brasilsp.pdf">Assembly Protocol Biobrick Device 1</a>
 
-
 
-
 
-
<td colspan="2" align="center">
 
-
<img src="https://static.igem.org/mediawiki/2014/d/d0/Device1_measurement_Brasil-SP.png" width="330" height="auto">
 
-
 
-
 
-
 
-
<tr>
 
-
 
-
 
-
<tr>
 
-
<br>
 
-
<td colspan="2" align="center">
 
-
 
-
<p align="center"> Biobrick device 2: BBa_J23101 + BBa_E0240, a new device built by each team  </p>
 
-
<br>
 
-
 
-
<a href="https://static.igem.org/mediawiki/2014/d/d3/Assembly_Biobrick_Devices_2_and_3_Measurement_Interlab_Study.pdf"> Assembly Protocol Biobrick Device 2 and 3 </a>
 
-
 
-
<br>
 
-
 
-
<td colspan="2" align="center">
 
-
<img src="https://static.igem.org/mediawiki/2014/8/84/Device2_measurement_brasilsp.png" width="350" height="auto">
 
-
<br>
 
-
<br>
 
-
 
-
 
-
<tr>
 
-
 
-
<td colspan="2" align="center">
 
-
 
-
<p align="center"> Biobrick device 3: BBa_J23115 + BBa_E0240, a new device built by each team.</p>
 
-
<br>
 
-
 
-
<a href="https://static.igem.org/mediawiki/2014/d/d3/Assembly_Biobrick_Devices_2_and_3_Measurement_Interlab_Study.pdf"> Assembly Protocol Biobrick Device 2 and 3 </a>
 
-
 
-
 
-
<td colspan="2" align="center">
 
-
<img src="https://static.igem.org/mediawiki/2014/9/93/Device3_measurement_brasilsp.png" width="350" height="auto">
 
-
 
-
<tr>
 
</td>
</td>
-
<td>
+
</html>
-
<ul>
+
-
    <li><a href="https://static.igem.org/mediawiki/2014/4/40/Interlab_Study_Measurement_Flow_Cytometer_Brasil-SP_Team.pdf">Flow Cytometry Protocol-Samples Preparation</a></li>
+
-
</ul>
+
-
<tr>
+
-
</td>
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
<!--end characterization protocols-->
+
{{:Team:Brasil-SP/Templates/Footer}}

Latest revision as of 23:38, 17 October 2014

Sponsors

This project is part of iGEM 2014

Wiki Links

Recent Changes Related Changes Special Pages Privacy Policy What Links Here Upload File My Preferences Disclaimers

Retrieved from "http://2014.igem.org/Team:Brasil-SP/Notebook/Protocols"