Team:British Columbia/Notebook/Protocol/Biobrickdigest

From 2014.igem.org

(Difference between revisions)
(Created page with "> {{:Team:British_Columbia/Templates/MainHeader}} <html> <div class="container"> <h1>BioBrick Restriction Digest</h1> <h2>Supplies:</h2> <ul> <li>dsH2O</li> <li>10x buffer...")
 
(4 intermediate revisions not shown)
Line 28: Line 28:
<li>Make a master mix with everything except the DNA. Keep everything on ice.</li>
<li>Make a master mix with everything except the DNA. Keep everything on ice.</li>
-
https://static.igem.org/mediawiki/2013/9/92/DigestA.JPG
+
<img src= "https://static.igem.org/mediawiki/2013/9/92/DigestA.JPG">
-
 
+
-
*depending on DNA concentration*
+
 +
<br>*depending on DNA concentration*
 +
<br>
 +
<br>
<li>Pipet aliquots to each sample tube and add DNA.</li>
<li>Pipet aliquots to each sample tube and add DNA.</li>
-
 
+
<br>
<li>Incubate at 37°C for 2-3h or let digest over night at room temperature.</li>
<li>Incubate at 37°C for 2-3h or let digest over night at room temperature.</li>
-
 
+
<br>
 +
<br>
</ol>
</ol>
</div>
</div>
</html>
</html>

Latest revision as of 23:29, 17 October 2014

> 2014 UBC iGEM



BioBrick Restriction Digest

Supplies:

  • dsH2O
  • 10x buffer (1,2,3,4)
  • 100x BSA
  • Restriction Enzymes (EcoRI, Xbal, SpeI, PstI)
  • DNA

Steps:

  1. Make a master mix with everything except the DNA. Keep everything on ice.

    2. *depending on DNA concentration*

  2. Pipet aliquots to each sample tube and add DNA.

  3. Incubate at 37°C for 2-3h or let digest over night at room temperature.


Retrieved from "http://2014.igem.org/Team:British_Columbia/Notebook/Protocol/Biobrickdigest"