Team:British Columbia/Notebook/Protocol

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           <h1>Protocols</h1>
           <h1>Protocols</h1>
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     <li>Item</li>
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     <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/PCR">PCR</a> </font> </b>
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===[[Team:British_Columbia/Notebook/Protocols/PCR|PCR]]===
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Instruction for colony PCR and regular PCR
Instruction for colony PCR and regular PCR
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<br></br>
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    <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocol/gelverification"> Gel Verification
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</a> </font> </b>
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    Make a gel and conduct gel elecrophoresis
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===[[Team:British_Columbia/Notebook/Protocols/gelverification|Gel Verification]]===
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Make a gel and conduct gel elecrophoresis
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===[[Team:British_Columbia/Notebook/Protocols/Biobrickdigest|BioBrick Restriction Digest]]===
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocol/Biobrickdigest">BioBrick Restriction Digest</a> </font> </b>
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Restriction digest with BioBrick plasmid an inserts
Restriction digest with BioBrick plasmid an inserts
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===[[Team:British_Columbia/Notebook/Protocols/ligation|BioBrick Ligation]]===
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/ligation">BioBrick Ligation</a> </font> </b>
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</li>
Ligate Biobrick insert to vector
Ligate Biobrick insert to vector
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===[[Team:British_Columbia/Notebook/Protocols/plates|Making Plates]]===
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/plates">Making Plates</a> </font> </b>
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Make LB plates with antibiotics
Make LB plates with antibiotics
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===[[Team:British_Columbia/Notebook/Protocols/media|Liquid media]]===
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/media">Liquid media</a> </font> </b>
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Make liquid LB media
Make liquid LB media
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===[[Team:British_Columbia/Notebook/Protocols/compcells|Competent cells]]===
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/compcells">Competent cells</a> </font> </b>
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Preparation of chemically competent cells
Preparation of chemically competent cells
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===[[Team:British_Columbia/Notebook/Protocols/transformation|Transformation]]===
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/transformation">Transformation</a> </font> </b>
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Transformation of plasmi DNA into chemically competent cells
Transformation of plasmi DNA into chemically competent cells
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===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]===
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly ">CRISPR Repeat-Spacer Array Assembly</a> </font> </b>
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Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
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===[[Team:British_Columbia/Notebook/Protocols/AnnealedOligonucleotides|Assembling Parts from Oligonucleotides]]===
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/AnnealedOligonucleotides">Assembling Parts from Oligonucleotides</a> </font> </b>
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Assemble short parts from annealed oligonucleotides
Assemble short parts from annealed oligonucleotides
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===[[Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis|(Multi) Site Directed Mutagenesis]]===
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis">(Multi) Site Directed Mutagenesis</a> </font> </b>
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Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
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===[[Team:British_Columbia/Notebook/Protocols/T4PNK|Oligonucleotide Phosphorylation]]===
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/T4PNK">Oligonucleotide Phosphorylation</a> </font> </b>
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</li>
Phosphorylate oligonucleotides prior to ligation
Phosphorylate oligonucleotides prior to ligation
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/ElectrocompCells">Electrocompetent Cell Production</a> </font> </b>
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===[[Team:British_Columbia/Notebook/Protocols/ElectrocompCells|Electrocompetent Cell Production]]===
 
Make electrocompetent cells
Make electrocompetent cells
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===[[Team:British_Columbia/Notebook/Protocols/CombinatorialLibraries|Make Combinatorial Part Libraries]]===
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/CombinatorialLibraries">Make Combinatorial Part Libraries</a> </font> </b>
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</li>
Make combinatorial part libraries from compatible parts
Make combinatorial part libraries from compatible parts
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Latest revision as of 23:26, 17 October 2014

2014 UBC iGEM

Protocols


Retrieved from "http://2014.igem.org/Team:British_Columbia/Notebook/Protocol"