Team:Penn/Protocol

From 2014.igem.org

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<h3>AMB-1 Specific Protocols</h3>
<h3>AMB-1 Specific Protocols</h3>
<p> Our protocols for working with AMB-1 have been included in this spec sheet. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.</p>
<p> Our protocols for working with AMB-1 have been included in this spec sheet. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.</p>
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<a href="https://2014.igem.org/Team:Penn/Specsheet" style = "font-size: 15px; margin:0 auto; color:#fff"> Click here to view the Spec Sheet! </a></br>
<p><a href="https://static.igem.org/mediawiki/2014/e/ea/Glycerol_Stock_of_Anaerobic_AMB-1.pdf">Glycerol Stock</a></p>
<p><a href="https://static.igem.org/mediawiki/2014/e/ea/Glycerol_Stock_of_Anaerobic_AMB-1.pdf">Glycerol Stock</a></p>

Revision as of 23:25, 17 October 2014

University of Pennsylvania iGEM

Protocols

Miniprep Cloning PCR Colony PCR Gel Extraction PCR Cleanup Restriction Enzyme Digest Ligation Transformation Inoculation E.Coli Specific Protocols

Gel Extraction

AMB-1 Specific Protocols

Our protocols for working with AMB-1 have been included in this spec sheet. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.

Click here to view the Spec Sheet!

Glycerol Stock

MSGM Media Preparation

EMSGM Media Preparation

Inoculation of Aerobic Cultures

Counting Cell Cultures

Transformation Protocol 1

Transformation Protocol 2

Penn iGEM Transformation Protocol

Plating Techniques