Team:Penn/Protocol

From 2014.igem.org

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Revision as of 23:24, 17 October 2014

University of Pennsylvania iGEM

Protocols

Miniprep Cloning PCR Colony PCR Gel Extraction PCR Cleanup Restriction Enzyme Digest Ligation Transformation Inoculation E.Coli Specific Protocols

Gel Extraction

AMB-1 Specific Protocols

Our protocols for working with AMB-1 have been included in this spec sheet. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.

Glycerol Stock

MSGM Media Preparation

EMSGM Media Preparation

Inoculation of Aerobic Cultures

Counting Cell Cultures

Transformation Protocol 1

Transformation Protocol 2

Penn iGEM Transformation Protocol

Plating Techniques

@@ Line 73: / Line 73: @@
 <p> Our protocols for working with AMB-1 have been included in this spec sheet. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.</p>
-<p><a href="">Inoculation</a></p>
+<p><a href="https://static.igem.org/mediawiki/2014/e/ea/Glycerol_Stock_of_Anaerobic_AMB-1.pdf">Glycerol Stock</a></p>
-<p><a href="">Inoculation Long-Term Protocol</a></p>
+<p><a href="https://static.igem.org/mediawiki/2014/a/a0/MSGM_Media_Preparation.pdf">MSGM Media Preparation </a></p>
-<p><a href="">MSGM Media Preparation </a></p>
+<p><a href="https://static.igem.org/mediawiki/2014/5/5e/EMSGM_Media_Preparation.pdf">EMSGM Media Preparation</a></p>
-<p><a href="">EMSGM Media Preparation</a></p>
+<p><a href="https://static.igem.org/mediawiki/2014/8/8c/Inoculation.pdf">Inoculation of Aerobic Cultures</a></p>
-<p><a href="">Inoculation of Aerobic Cultures</a></p>
+<p><a href="https://static.igem.org/mediawiki/2014/a/a3/Counting_Cell_Cultures.pdf">Counting Cell Cultures</a></p>
-<p><a href="">Counting Cell Cultures</a></p>
+<p><a href="https://static.igem.org/mediawiki/2014/e/ef/Transformation_Protocol_1.pdf">Transformation Protocol 1</a></p>
-<p><a href="">Transformation Protocol 1</a></p>
+<p><a href="https://static.igem.org/mediawiki/2014/9/9a/Transformation_Protocol_2.pdf">Transformation Protocol 2</a></p>
-<p><a href="">Transformation Protocol 2</a></p>
+<p><a href="https://static.igem.org/mediawiki/2014/7/77/Penn_iGEM_Transformation_Protocol.pdf">Penn iGEM Transformation Protocol</a></p>
-<p><a href="">Penn iGEM Transformation Protocol</a></p>
+<p><a href="https://static.igem.org/mediawiki/2014/5/5d/Plating_Techniques.pdf">Plating Techniques</a></p>
-<p><a href="">Plating Techniques</a></p>