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Team:SYSU-China/file/Project/Design/Overview.html
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<p>M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An <em>E. coli</em> with pBT-Bait and pRPT-gⅧ. At the temperature of 0 degree Celcius</p> | <p>M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An <em>E. coli</em> with pBT-Bait and pRPT-gⅧ. At the temperature of 0 degree Celcius</p> | ||
| - | < | + | <a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/8/85/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE1_new.png"><img src="https://static.igem.org/mediawiki/2014/8/85/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE1_new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> |
<p>2.Infection</p> | <p>2.Infection</p> | ||
<p>When the temperature rise to 30 degree Celcius, the M13 vector infects into the <em>E. coli</em>.</p> | <p>When the temperature rise to 30 degree Celcius, the M13 vector infects into the <em>E. coli</em>.</p> | ||
| - | < | + | <a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/2/21/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE2new.png"><img src="https://static.igem.org/mediawiki/2014/2/21/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE2new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> |
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<p>3.Mutagenesis</p> | <p>3.Mutagenesis</p> | ||
<p>When the M13 vector undergo replication, a library of different Prey is generated.</p> | <p>When the M13 vector undergo replication, a library of different Prey is generated.</p> | ||
| - | < | + | <a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/7/7f/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE3new.png"><img src="https://static.igem.org/mediawiki/2014/7/7f/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE3new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> |
<p>4.Selection</p> | <p>4.Selection</p> | ||
<p>Various Prey undergo selection processes with the help of Bacteria Two-Hybrid system</p> | <p>Various Prey undergo selection processes with the help of Bacteria Two-Hybrid system</p> | ||
| - | < | + | <a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/f/f2/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE4new.png"><img src="https://static.igem.org/mediawiki/2014/f/f2/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE4new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> |
<p>5.Temperature regulated amplification</p> | <p>5.Temperature regulated amplification</p> | ||
<p>The temperature rise to 37 degree Celcius, so that mRNA of gⅧ can be translated into p8</p> | <p>The temperature rise to 37 degree Celcius, so that mRNA of gⅧ can be translated into p8</p> | ||
| - | < | + | <a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/6/6d/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE5new.png"><img src="https://static.igem.org/mediawiki/2014/6/6d/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE5new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> |
<p>6. Rescue and Evolved</p> | <p>6. Rescue and Evolved</p> | ||
<p>The p8 compensate M13 deficient, so that progeny phage can be generated</p> | <p>The p8 compensate M13 deficient, so that progeny phage can be generated</p> | ||
| - | < | + | <a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/f/f6/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE6new.png"><img src="https://static.igem.org/mediawiki/2014/f/f6/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE6new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> |
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<!--SYSUCHINA!--> | <!--SYSUCHINA!--> | ||
Revision as of 23:07, 17 October 2014
Overview
In order to achieve the goal of integrating the directed evolution processes into a single automatic and controllable system, we come up with the following design.
1.The initiate state
M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An E. coli with pBT-Bait and pRPT-gⅧ. At the temperature of 0 degree Celcius
<a class="fancybox" rel="group" href="
"><img src="" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a>
2.Infection
When the temperature rise to 30 degree Celcius, the M13 vector infects into the E. coli.
<a class="fancybox" rel="group" href="
"><img src="" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a>
3.Mutagenesis
When the M13 vector undergo replication, a library of different Prey is generated.
<a class="fancybox" rel="group" href="
"><img src="" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a>
4.Selection
Various Prey undergo selection processes with the help of Bacteria Two-Hybrid system
<a class="fancybox" rel="group" href="
"><img src="" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a>
5.Temperature regulated amplification
The temperature rise to 37 degree Celcius, so that mRNA of gⅧ can be translated into p8
<a class="fancybox" rel="group" href="
"><img src="" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a>
6. Rescue and Evolved
The p8 compensate M13 deficient, so that progeny phage can be generated
<a class="fancybox" rel="group" href="
"><img src="" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a>
