Team:ITESM-CEM/Project/Data
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<sub2></sub2><sub2></sub2><sub2></sub2> | <sub2></sub2><sub2></sub2><sub2></sub2> | ||
- | <sub2><a href="#One" style="color: #FFF;"> | + | <sub2><a href="#One" style="color: #FFF;">PCR's</a></sub2> |
<sub2><a href="#Two" style="color: #FFF;">Two</a></sub2> | <sub2><a href="#Two" style="color: #FFF;">Two</a></sub2> | ||
<sub2><a href="#Three" style="color: #FFF;">Three</a></sub2> | <sub2><a href="#Three" style="color: #FFF;">Three</a></sub2> | ||
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<p>If you choose to create a model during your project, please write about it here. Modeling is not an essential part of iGEM, but we encourage any and all teams to model some aspect of their project. See previous "Best Model" awards for more information.<br><br></p> | <p>If you choose to create a model during your project, please write about it here. Modeling is not an essential part of iGEM, but we encourage any and all teams to model some aspect of their project. See previous "Best Model" awards for more information.<br><br></p> | ||
- | <a name="One"><h2> | + | <a name="One"><h2>PCR's for gene isolation</h2></a> |
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+ | <p><pie><b>Gel 1.</b>High Fidelity PCRs Electrophoresis Gel . Well content: 2) NeoR PCR(786pb) 3)CMV Promoter PCR(588bp) 4) BGHPA PCR (228bp).</p></pie><br> | ||
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+ | <p>imagen</p><br> | ||
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+ | <h4>Biobricks in plasmid psB1C3</h4> | ||
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+ | <h4>BGHPA</h4> | ||
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+ | <p style="text-align: justify; text-justify: inter-word;">The ligation (view Material and Methods) of BGHPA was made with J04450 (psB1C3 with RFP protein). The BB_J04450 by itself produces red colonies and grows in the antibiotic Cloramphenicol. After the ligation and transformation, only the white colonies were selected. An extraction from a white colony growing on 50ml LB Cam+ was made in order to perform gel electrophoresis as shown in figure B.</p><br> | ||
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+ | <p>imagen</p><br> | ||
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+ | <p><pie><b>Figure A.</b></p> Isolated BGHPA transformed colony.</pie><br> | ||
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+ | <p>imagen</p><br> | ||
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+ | <p><pie><b>Figure B.</b> BGHPA in plasmid psB1C3 gel electrophoresis. Lane 2. </p></pie><br> | ||
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+ | <h4>CMV</h4> | ||
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+ | <p style="text-align: justify; text-justify: inter-word;">The ligation (view material and methods) of CMV was made with J04450 (psB1C3 with RFP protein). The BB_J04450 by itself produces red colonies and grows in the antibiotic Cloramphenicol. After the ligation and transformation, only the white colonies were selected. An extraction from a white colony growing on 50ml LB Cam+ was made in order to perform gel electrophoresis as shown in figure B.</p><br> | ||
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+ | <p>imagen</p><br> | ||
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+ | <p><pie><b>Figure A.</b></p> Isolated CMV transformed colony.</pie><br> | ||
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+ | <p>imagen</p><br> | ||
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+ | <p><pie><b>Figure B.</b> CMV in plasmid psB1C3 gel electrophoresis. Lanes 1-3. </p></pie><br> | ||
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+ | <h4>NeoR</h4> | ||
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+ | <p style="text-align: justify; text-justify: inter-word;">The ligation (view material and methods) of NeoR was made with J04450 (psB1C3 with RFP protein). The BB_J04450 by itself produces red colonies and grows in the antibiotic Cloramphenicol. After the ligation and transformation 8 (Figure A), only the white colonies were selected. An extraction from a white colony growing on 50ml LB Cam+ was made in order to perform gel electrophoresis as shown in Figure B. On lane 8, NeoR extraction is shown with the three bands isoforms, they are barely visible because the plasmid extraction was diluted 5 fold.</p><br> | ||
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+ | <p>imagen</p><br> | ||
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+ | <p><pie><b>Figure A.</b></p> Isolated NeoR transformed colony.</pie><br> | ||
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+ | <p>imagen</p><br> | ||
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+ | <p><pie><b>Figure B.</b> NeoR in plasmid psB1C3 gel electrophoresis.<br> Lanes: 2-4. NeoR in psB1C3 digestion with XhoI. <br> 6-8. NeoR in psB1C3. <br> 10-12. CMV in psB1C3. <br> </p></pie><br> | ||
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<gotop><a href="#top">Back to top ↑</a></gotop><br><br> | <gotop><a href="#top">Back to top ↑</a></gotop><br><br> | ||
Revision as of 22:58, 17 October 2014
ITESM-CEM | Enzy7-K me |
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