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Team:Paris Saclay/Notebook/July/16
From 2014.igem.org
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* BBa J23119 | * BBa J23119 | ||
| + | |||
| + | '''Samples:''' | ||
| + | |||
| + | # Concentrated | ||
| + | # Undiluted | ||
| + | # Diluted 10^-1 | ||
| + | # Control | ||
| + | |||
| + | '''Results:''' | ||
| + | |||
| + | # Concentrated: 99 colonies | ||
| + | # Undiluted: 5 colonies | ||
| + | # Diluted: 0 | ||
| + | # Control: 0 | ||
| + | |||
| + | '''Conclusion:''' | ||
| + | |||
| + | Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures. | ||
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | ||
Revision as of 10:02, 17 July 2014
Contents |
Wednesday 16th July
Lab work
1 - Transformation in DH5α
by Fabio, Maher and Romain
We transformed in DH5α the following Biobrick:
- BBa J23119
Samples:
- Concentrated
- Undiluted
- Diluted 10^-1
- Control
Results:
- Concentrated: 99 colonies
- Undiluted: 5 colonies
- Diluted: 0
- Control: 0
Conclusion:
Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures.
Reunion
Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning)
Reunion: Lab meeting to discuss the project. (afternoon)
Meeting with Evry's Team. (night) Arnaud represents us
