Team:British Columbia/Notebook/Protocol

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          <h1>Protocols</h1>
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Revision as of 22:48, 17 October 2014



  • <a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/PCR">PCR</a>

    • Instruction for colony PCR and regular PCR
    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/gelverificaation">Gel Verification</a> 
    •    Make a gel and conduct gel elecrophoresis


    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/Biobrickdigest">BioBrick Restriction Digest</a>

    • Restriction digest with BioBrick plasmid an inserts
    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/ligation">BioBrick Ligation</a>

    • Ligate Biobrick insert to vector
    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/plates">Making Plates</a>

    • Make LB plates with antibiotics
    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/media">Liquid media</a>

    • Make liquid LB media
    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/compcells">Competent cells</a>

    • Preparation of chemically competent cells
    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/transformation">Transformation</a>

    • Transformation of plasmi DNA into chemically competent cells
    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly ">CRISPR Repeat-Spacer Array Assembly</a>

    • Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/AnnealedOligonucleotides">Assembling Parts from Oligonucleotides</a>

    • Assemble short parts from annealed oligonucleotides
    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis">(Multi) Site Directed Mutagenesis</a>

    • Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/T4PNK">Oligonucleotide Phosphorylation</a>

    • Phosphorylate oligonucleotides prior to ligation
    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/ElectrocompCells">Electrocompetent Cell Production</a>

    • Make electrocompetent cells
    </br>

  • <a href="Team:British_Columbia/Notebook/Protocols/CombinatorialLibraries">Make Combinatorial Part Libraries</a>

    • Make combinatorial part libraries from compatible parts
    </br>


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