Team:Oxford/Results
From 2014.igem.org
(Difference between revisions)
AndyRussell (Talk | contribs) |
|||
Line 58: | Line 58: | ||
<a href="#hide5" class="hide" id="hide5"><div class="modelling"> | <a href="#hide5" class="hide" id="hide5"><div class="modelling"> | ||
- | <h1white> | + | <h1white>1. purify the pUNI-ABTUNJK and pSB1C3-ABTUNJK plasmids, which encode the microcompartments</h1white></div></a> |
+ | <div class="list"> | ||
+ | <div class="white_news_block2"> | ||
+ | <h1blue2>Calculating the pH change</h1blue2> | ||
+ | |||
+ | <br><br> | ||
+ | We then used our model to predict the effect on the system if you simply increase the amount of water in the aqueous layer. | ||
+ | This shows how much water is necessary to prevent the pH from dropping too much. It demonstrates why addition of a buffer is the more reasonable choice to control the pH of the system. | ||
+ | |||
+ | <br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/6/6c/Oxford_DCMdeg18.png" style="float:left;position:relative; width:60%; margin-left:20%; margin-right:20%;margin-bottom:2%;" /> | ||
+ | |||
+ | The graph here is for non specific inputs and is for demonstration purposes only. It shows well how the model responds to changing the input values. | ||
+ | <br><br> | ||
+ | <u></u> | ||
+ | |||
+ | <a href="https://2014.igem.org/Team:Oxford/how_much_can_we_degrade#hide4">Buffers? | ||
+ | </a> | ||
+ | |||
+ | <a href="#hide5" class="hide" id="hide5"><div class="modelling"> | ||
+ | <h1white>2. verify expression of pUNI-ABTUNJK plasmids in E. coli using Western blotting</h1white></div></a> | ||
<div class="list"> | <div class="list"> | ||
<div class="white_news_block2"> | <div class="white_news_block2"> |
Revision as of 22:44, 17 October 2014