"
Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0034 mRFP Assay
From 2014.igem.org
(Difference between revisions)
| Line 38: | Line 38: | ||
<div class="ldd_submenu" style="left:-415px; width:970px;"> | <div class="ldd_submenu" style="left:-415px; width:970px;"> | ||
<ul style="margin-left:20px;"> | <ul style="margin-left:20px;"> | ||
| - | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/ | + | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Background">Overview</a></li> |
| - | + | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Background">Background</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Background">Background</a></li> | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Achievements">Achievements</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Achievements">Achievements</a></li> | ||
Revision as of 22:43, 17 October 2014
Notebook
Lab Documents
140910 asB0034&B0034 mRFP Assay
-
Start
-
Transformation
Ptrc-stem-asB0034-stem on pHN1257&Ptet-B0034-mRFP-dt on pSB6A1 on ice 30min each of 2.5µL DNA to DH5α 42℃ 30sec on ice 2min -
Colony PCR
Ptrc-stem-asB0034-stem(pHN1257)&Ptet-B0034-mRFP-dT(pSB6A1) pHN1257_up_F Tm:60.1℃ asB0034-R Tm:68.7℃ -
Preculture
Ptrc-stem-asB0034-stem(pHN1257)&Ptet-B0034-mRFP-dT(pSB6A1) LB 2 mL for 3hours Turbidity was controlled 0.1 -
Culture
LB 2 mL IPTG 20 µ or not for 11 hours, 13hours, 15hours, 17 hours -
Assay
fluoresence imageing 514 nm 610 filter -
Complete!
