Team:WPI-Worcester

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<h1>A Summary of our Project</h1>
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<a href="http://www.wpi.edu/news/20134/igemteam.html">
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<h1>Women in Science Outreach</h1>
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<a href="https://2014.igem.org/Team:WPI-Worcester/About">About this wiki!</a>
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 +
</br>
 +
<h4>Ag-goat-ination:</h4><p><h8>Combating Antibiotic Resistance through Diagnostic Agglutination Assays</h8></p><p>Just this year, in a vote held for the Longitude Prize, antibiotic resistance was voted the most pressing issue of our time. One of the biggest causes of antibiotic resistance lies in the farming industry; more than 3 times the amount of antibiotics given to humans are administered to livestock. Over-prescription of antibiotics promotes resistance in both livestock pathogens and natural flora, which can be transmitted to humans through meat and other products.</p>
 +
<p>A cheap, quick, and reliable mechanism for detecting and identifying infection is one solution to this problem. We developed an E.coli based cell surface antigen expression system that can be utilized as a diagnostic tool for nearly any viral, bacterial, or fungal infection. The recombinant E.coli are utilized in a direct agglutination test using the host organism’s serum. Our team successfully demonstrated the feasibility of this diagnostic tool using a GFP antibody and antigen pair. </p>
 +
<p><center><a href="https://2014.igem.org/Team:WPI-Worcester/Medal-Fulfillment"><img src="https://static.igem.org/mediawiki/2014/1/1a/WPI_MedalsGraphic.png"/></a></center></p>
 +
<p><center><h9>Click here to view our medal fulfullment checklist!</h9></center></p>
 +
<p><center><img src="https://static.igem.org/mediawiki/2014/thumb/a/ac/WPI_igem_Team_photo_2014.png/800px-WPI_igem_Team_photo_2014.png"/></center></p>
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Latest revision as of 22:42, 17 October 2014

Team:WPI-Worcester - 2014.igem.org

 

Team:WPI-Worcester

From 2014.igem.org


Ag-goat-ination:

Combating Antibiotic Resistance through Diagnostic Agglutination Assays

Just this year, in a vote held for the Longitude Prize, antibiotic resistance was voted the most pressing issue of our time. One of the biggest causes of antibiotic resistance lies in the farming industry; more than 3 times the amount of antibiotics given to humans are administered to livestock. Over-prescription of antibiotics promotes resistance in both livestock pathogens and natural flora, which can be transmitted to humans through meat and other products.

A cheap, quick, and reliable mechanism for detecting and identifying infection is one solution to this problem. We developed an E.coli based cell surface antigen expression system that can be utilized as a diagnostic tool for nearly any viral, bacterial, or fungal infection. The recombinant E.coli are utilized in a direct agglutination test using the host organism’s serum. Our team successfully demonstrated the feasibility of this diagnostic tool using a GFP antibody and antigen pair.

Click here to view our medal fulfullment checklist!




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