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Team:SYSU-China/file/Project/Notebook/Labnote/Mutation.html
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| - | + | <!--SYSUCHINA--> | |
| + | <h1>Mutation·LABNOTE</h1> | ||
| + | <p><b>May:</b> Amplify biobricks. </p> | ||
| + | <p><b>June:</b> Clone the WT DNAQ ,recA,umuC,umuD from <em>E.coli</em>. </p> | ||
| + | <p><b>July:</b> Perform the point-mutation and truncation of the four genes.</p> | ||
| + | <p><b>August:</b> Add prefix and suffix to the genes;</p> | ||
| + | <p>            Add RBS to the genes.</p> | ||
| + | <p><b>September:</b> Construct the polycistron plasmid </p> | ||
| + | <p>    (DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;DNAQ926+umuD’+umuC+recA730;); | ||
| + | <p>            Add promoter to construct the expression vector; | ||
| + | <p>            Test the mutation rate.</p> | ||
| + | <p><b>October:</b> adapt the testing method and test the mutation rates.</p> | ||
| + | <p>            Prepare the biobrick for submission. </p> | ||
| + | |||
| + | <!--SYSUCHINA!--> | ||
Latest revision as of 22:30, 17 October 2014
Mutation·LABNOTE
May: Amplify biobricks.
June: Clone the WT DNAQ ,recA,umuC,umuD from E.coli.
July: Perform the point-mutation and truncation of the four genes.
August: Add prefix and suffix to the genes;
            Add RBS to the genes.
September: Construct the polycistron plasmid
    (DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;DNAQ926+umuD’+umuC+recA730;); <p>            Add promoter to construct the expression vector; <p>            Test the mutation rate.
October: adapt the testing method and test the mutation rates.
            Prepare the biobrick for submission.
