Team:Oxford/modellingA

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(Difference between revisions)
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+
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+
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+
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+
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+
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+
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+
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+
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-
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+
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+
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+
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+
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-
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+
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-
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+
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-
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+
     td.td26 {width: 49.0px; height: 11.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
-
    
+
     td.td27 {width: 42.0px; height: 11.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
-
</style>
+
    td.td28 {width: 66.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
 +
     td.td29 {width: 69.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
 +
   </style>
</head>
</head>
Line 63: Line 64:
<p class="p1"><br></p>
<p class="p1"><br></p>
 +
<table cellspacing="0" cellpadding="0" class="t1">
<table cellspacing="0" cellpadding="0" class="t1">
   <tbody>
   <tbody>
Line 70: Line 72:
       </td>
       </td>
       <td valign="top" class="td2">
       <td valign="top" class="td2">
-
         <p class="p2"><span class="s1">p(DCM) /kPa</span></p>
+
         <p class="p2"><span class="s1">V(DCM)</span><span class="s2"><sub> </sub></span><span class="s1">added (± 5E-4) /ml</span></p>
       </td>
       </td>
       <td valign="top" class="td3">
       <td valign="top" class="td3">
-
         <p class="p2"><span class="s1">n</span><span class="s2"><sub>(g)</sub></span><span class="s1"> /mol</span></p>
+
         <p class="p2"><span class="s1">n(DCM)</span><span class="s2"><sub>(l)</sub></span><span class="s1"> unadjusted (± 8E-6) /mol</span></p>
       </td>
       </td>
       <td valign="top" class="td4">
       <td valign="top" class="td4">
-
         <p class="p2"><span class="s1">V(DCM)</span><span class="s2"><sub> </sub></span><span class="s1">added /ml</span></p>
+
         <p class="p2"><span class="s1">[DCM] unadjusted (± 2E-3) /M</span></p>
       </td>
       </td>
       <td valign="top" class="td5">
       <td valign="top" class="td5">
-
         <p class="p2"><span class="s1">n(DCM)</span><span class="s2"><sub>(l)</sub></span><span class="s1"> unadjusted /mol</span></p>
+
         <p class="p2"><span class="s1">p(DCM)<span class="Apple-converted-space"> </span></span></p>
 +
        <p class="p2"><span class="s1">(± 4E-2) /kPa</span></p>
       </td>
       </td>
-
       <td valign="top" class="td1">
+
       <td valign="top" class="td5">
-
         <p class="p2"><span class="s1">[DCM] unadjusted /M</span></p>
+
         <p class="p2"><span class="s1">n(DCM)</span><span class="s2"><sub>(g)</sub></span><span class="s1"><span class="Apple-converted-space"> </span></span></p>
 +
        <p class="p2"><span class="s1">(± 4E-7) /mol</span></p>
       </td>
       </td>
       <td valign="top" class="td6">
       <td valign="top" class="td6">
-
         <p class="p2"><span class="s1">n(DCM)</span><span class="s2"><sub>(l)</sub></span><span class="s1"> adjusted /mol</span></p>
+
         <p class="p2"><span class="s1">n(DCM)</span><span class="s2"><sub>(l)</sub></span><span class="s1"> (± 9E-6) adjusted /mol</span></p>
       </td>
       </td>
       <td valign="top" class="td7">
       <td valign="top" class="td7">
-
         <p class="p2"><span class="s1">[DCM] adjusted /M</span></p>
+
         <p class="p2"><span class="s1">[DCM] adjusted (± 2E-3) /M</span></p>
       </td>
       </td>
       <td valign="top" class="td8">
       <td valign="top" class="td8">
 +
        <p class="p2"><span class="s1">Δ[DCM] /M</span></p>
 +
      </td>
 +
      <td valign="top" class="td9">
         <p class="p2"><span class="s1">[DCM] % deviation</span></p>
         <p class="p2"><span class="s1">[DCM] % deviation</span></p>
       </td>
       </td>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <td valign="top" class="td9">
+
       <td valign="top" class="td10">
         <p class="p2"><span class="s1"><b>(30°C, 5mM)</b></span></p>
         <p class="p2"><span class="s1"><b>(30°C, 5mM)</b></span></p>
-
      </td>
 
-
      <td valign="top" class="td10">
 
-
        <p class="p3"><span class="s1">1.56E-04</span></p>
 
       </td>
       </td>
       <td valign="top" class="td11">
       <td valign="top" class="td11">
-
         <p class="p3"><span class="s1">3.94E-12</span></p>
+
         <p class="p3"><span class="s1">6.4E-03</span></p>
       </td>
       </td>
       <td valign="top" class="td12">
       <td valign="top" class="td12">
-
         <p class="p3"><span class="s1">6.4E-03</span></p>
+
         <p class="p3"><span class="s1">1.0E-04</span></p>
       </td>
       </td>
       <td valign="top" class="td13">
       <td valign="top" class="td13">
-
        <p class="p3"><span class="s1">1.0E-04</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td9">
 
         <p class="p3"><span class="s1">2.0E-02</span></p>
         <p class="p3"><span class="s1">2.0E-02</span></p>
       </td>
       </td>
       <td valign="top" class="td14">
       <td valign="top" class="td14">
-
         <p class="p3"><span class="s1">1.0E-04</span></p>
+
        <p class="p3"><span class="s1">6.2E-01</span></p>
 +
      </td>
 +
      <td valign="top" class="td14">
 +
         <p class="p3"><span class="s1">1.6E-08</span></p>
       </td>
       </td>
       <td valign="top" class="td15">
       <td valign="top" class="td15">
-
         <p class="p3"><span class="s1"><b>2.0E-02</b></span></p>
+
         <p class="p3"><span class="s1">1.0E-04</span></p>
       </td>
       </td>
       <td valign="top" class="td16">
       <td valign="top" class="td16">
-
         <p class="p3"><span class="s1">3.9E-06</span></p>
+
         <p class="p3"><span class="s1"><b>2.0E-02</b></span></p>
       </td>
       </td>
-
    </tr>
 
-
    <tr>
 
       <td valign="top" class="td17">
       <td valign="top" class="td17">
-
         <p class="p2"><span class="s1"><b>(30°C, 10mM)</b></span></p>
+
         <p class="p3"><span class="s1">3.1E-06</span></p>
       </td>
       </td>
       <td valign="top" class="td18">
       <td valign="top" class="td18">
-
         <p class="p3"><span class="s1">3.12E-04</span></p>
+
         <p class="p3"><span class="s1">1.6E-02</span></p>
       </td>
       </td>
 +
    </tr>
 +
    <tr>
       <td valign="top" class="td19">
       <td valign="top" class="td19">
-
         <p class="p3"><span class="s1">7.88E-12</span></p>
+
         <p class="p2"><span class="s1"><b>(30°C, 10mM)</b></span></p>
       </td>
       </td>
       <td valign="top" class="td20">
       <td valign="top" class="td20">
Line 139: Line 143:
         <p class="p3"><span class="s1">5.0E-05</span></p>
         <p class="p3"><span class="s1">5.0E-05</span></p>
       </td>
       </td>
-
       <td valign="top" class="td17">
+
       <td valign="top" class="td22">
         <p class="p3"><span class="s1">1.0E-02</span></p>
         <p class="p3"><span class="s1">1.0E-02</span></p>
       </td>
       </td>
-
       <td valign="top" class="td22">
+
       <td valign="top" class="td23">
-
         <p class="p3"><span class="s1">5.0E-05</span></p>
+
         <p class="p3"><span class="s1">3.1E-01</span></p>
       </td>
       </td>
       <td valign="top" class="td23">
       <td valign="top" class="td23">
-
         <p class="p3"><span class="s1"><b>1.0E-02</b></span></p>
+
         <p class="p3"><span class="s1">7.9E-09</span></p>
       </td>
       </td>
       <td valign="top" class="td24">
       <td valign="top" class="td24">
-
         <p class="p3"><span class="s1">1.6E-05</span></p>
+
        <p class="p3"><span class="s1">5.0E-05</span></p>
 +
      </td>
 +
      <td valign="top" class="td25">
 +
        <p class="p3"><span class="s1"><b>1.0E-02</b></span></p>
 +
      </td>
 +
      <td valign="top" class="td26">
 +
         <p class="p3"><span class="s1">1.6E-06</span></p>
 +
      </td>
 +
      <td valign="top" class="td27">
 +
        <p class="p3"><span class="s1">1.6E-02</span></p>
       </td>
       </td>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <td valign="top" class="td17">
+
       <td valign="top" class="td19">
         <p class="p2"><span class="s1"><b>(30°C, 20mM)</b></span></p>
         <p class="p2"><span class="s1"><b>(30°C, 20mM)</b></span></p>
-
      </td>
 
-
      <td valign="top" class="td18">
 
-
        <p class="p3"><span class="s1">6.24E-04</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td19">
 
-
        <p class="p3"><span class="s1">1.58E-11</span></p>
 
       </td>
       </td>
       <td valign="top" class="td20">
       <td valign="top" class="td20">
Line 168: Line 175:
         <p class="p3"><span class="s1">2.5E-05</span></p>
         <p class="p3"><span class="s1">2.5E-05</span></p>
       </td>
       </td>
-
       <td valign="top" class="td17">
+
       <td valign="top" class="td22">
         <p class="p3"><span class="s1">5.0E-03</span></p>
         <p class="p3"><span class="s1">5.0E-03</span></p>
       </td>
       </td>
-
       <td valign="top" class="td22">
+
       <td valign="top" class="td23">
-
         <p class="p3"><span class="s1">2.5E-05</span></p>
+
         <p class="p3"><span class="s1">1.6E-01</span></p>
       </td>
       </td>
       <td valign="top" class="td23">
       <td valign="top" class="td23">
-
         <p class="p3"><span class="s1"><b>5.0E-03</b></span></p>
+
         <p class="p3"><span class="s1">3.9E-09</span></p>
       </td>
       </td>
       <td valign="top" class="td24">
       <td valign="top" class="td24">
-
         <p class="p3"><span class="s1">6.3E-05</span></p>
+
         <p class="p3"><span class="s1">2.5E-05</span></p>
 +
      </td>
 +
      <td valign="top" class="td25">
 +
        <p class="p3"><span class="s1"><b>5.0E-03</b></span></p>
 +
      </td>
 +
      <td valign="top" class="td26">
 +
        <p class="p3"><span class="s1">7.9E-07</span></p>
 +
      </td>
 +
      <td valign="top" class="td27">
 +
        <p class="p3"><span class="s1">1.6E-02</span></p>
       </td>
       </td>
     </tr>
     </tr>
     <tr>
     <tr>
-
      <td valign="top" class="td9">
 
-
        <p class="p1"><br></p>
 
-
      </td>
 
       <td valign="top" class="td10">
       <td valign="top" class="td10">
         <p class="p1"><br></p>
         <p class="p1"><br></p>
       </td>
       </td>
-
       <td valign="top" class="td11">
+
       <td valign="top" class="td28">
         <p class="p1"><br></p>
         <p class="p1"><br></p>
       </td>
       </td>
-
       <td valign="top" class="td25">
+
       <td valign="top" class="td12">
         <p class="p1"><br></p>
         <p class="p1"><br></p>
       </td>
       </td>
Line 197: Line 210:
         <p class="p1"><br></p>
         <p class="p1"><br></p>
       </td>
       </td>
-
       <td valign="top" class="td9">
+
       <td valign="top" class="td14">
         <p class="p1"><br></p>
         <p class="p1"><br></p>
       </td>
       </td>
Line 203: Line 216:
         <p class="p1"><br></p>
         <p class="p1"><br></p>
       </td>
       </td>
-
       <td valign="top" class="td26">
+
       <td valign="top" class="td15">
         <p class="p1"><br></p>
         <p class="p1"><br></p>
       </td>
       </td>
-
       <td valign="top" class="td16">
+
       <td valign="top" class="td29">
         <p class="p1"><br></p>
         <p class="p1"><br></p>
       </td>
       </td>
-
    </tr>
 
-
    <tr>
 
       <td valign="top" class="td17">
       <td valign="top" class="td17">
-
         <p class="p2"><span class="s1"><b>(37°C, 5mM)</b></span></p>
+
         <p class="p1"><br></p>
       </td>
       </td>
       <td valign="top" class="td18">
       <td valign="top" class="td18">
-
         <p class="p3"><span class="s1">1.56E-04</span></p>
+
         <p class="p1"><br></p>
       </td>
       </td>
 +
    </tr>
 +
    <tr>
       <td valign="top" class="td19">
       <td valign="top" class="td19">
-
         <p class="p3"><span class="s1">3.94E-12</span></p>
+
         <p class="p2"><span class="s1"><b>(37°C, 5mM)</b></span></p>
       </td>
       </td>
       <td valign="top" class="td20">
       <td valign="top" class="td20">
Line 226: Line 239:
         <p class="p3"><span class="s1">1.0E-04</span></p>
         <p class="p3"><span class="s1">1.0E-04</span></p>
       </td>
       </td>
-
       <td valign="top" class="td17">
+
       <td valign="top" class="td22">
         <p class="p3"><span class="s1">2.0E-02</span></p>
         <p class="p3"><span class="s1">2.0E-02</span></p>
       </td>
       </td>
-
       <td valign="top" class="td22">
+
       <td valign="top" class="td23">
-
         <p class="p3"><span class="s1">1.0E-04</span></p>
+
         <p class="p3"><span class="s1">6.2E-01</span></p>
       </td>
       </td>
       <td valign="top" class="td23">
       <td valign="top" class="td23">
-
         <p class="p3"><span class="s1"><b>2.0E-02</b></span></p>
+
         <p class="p3"><span class="s1">1.5E-08</span></p>
       </td>
       </td>
       <td valign="top" class="td24">
       <td valign="top" class="td24">
-
         <p class="p3"><span class="s1">3.9E-06</span></p>
+
        <p class="p3"><span class="s1">1.0E-04</span></p>
 +
      </td>
 +
      <td valign="top" class="td25">
 +
        <p class="p3"><span class="s1"><b>2.0E-02</b></span></p>
 +
      </td>
 +
      <td valign="top" class="td26">
 +
         <p class="p3"><span class="s1">3.1E-06</span></p>
 +
      </td>
 +
      <td valign="top" class="td27">
 +
        <p class="p3"><span class="s1">1.5E-02</span></p>
       </td>
       </td>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <td valign="top" class="td17">
+
       <td valign="top" class="td19">
         <p class="p2"><span class="s1"><b>(37°C, 10mM)</b></span></p>
         <p class="p2"><span class="s1"><b>(37°C, 10mM)</b></span></p>
-
      </td>
 
-
      <td valign="top" class="td18">
 
-
        <p class="p3"><span class="s1">3.12E-04</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td19">
 
-
        <p class="p3"><span class="s1">7.88E-12</span></p>
 
       </td>
       </td>
       <td valign="top" class="td20">
       <td valign="top" class="td20">
Line 255: Line 271:
         <p class="p3"><span class="s1">5.0E-05</span></p>
         <p class="p3"><span class="s1">5.0E-05</span></p>
       </td>
       </td>
-
       <td valign="top" class="td17">
+
       <td valign="top" class="td22">
         <p class="p3"><span class="s1">1.0E-02</span></p>
         <p class="p3"><span class="s1">1.0E-02</span></p>
       </td>
       </td>
-
       <td valign="top" class="td22">
+
       <td valign="top" class="td23">
-
         <p class="p3"><span class="s1">5.0E-05</span></p>
+
         <p class="p3"><span class="s1">3.1E-01</span></p>
       </td>
       </td>
       <td valign="top" class="td23">
       <td valign="top" class="td23">
-
         <p class="p3"><span class="s1"><b>1.0E-02</b></span></p>
+
         <p class="p3"><span class="s1">7.7E-09</span></p>
       </td>
       </td>
       <td valign="top" class="td24">
       <td valign="top" class="td24">
-
         <p class="p3"><span class="s1">1.6E-05</span></p>
+
         <p class="p3"><span class="s1">5.0E-05</span></p>
 +
      </td>
 +
      <td valign="top" class="td25">
 +
        <p class="p3"><span class="s1"><b>1.0E-02</b></span></p>
 +
      </td>
 +
      <td valign="top" class="td26">
 +
        <p class="p3"><span class="s1">1.5E-06</span></p>
 +
      </td>
 +
      <td valign="top" class="td27">
 +
        <p class="p3"><span class="s1">1.5E-02</span></p>
       </td>
       </td>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <td valign="top" class="td9">
+
       <td valign="top" class="td10">
         <p class="p2"><span class="s1"><b>(37°C, 20mM)</b></span></p>
         <p class="p2"><span class="s1"><b>(37°C, 20mM)</b></span></p>
-
      </td>
 
-
      <td valign="top" class="td10">
 
-
        <p class="p3"><span class="s1">6.24E-04</span></p>
 
       </td>
       </td>
       <td valign="top" class="td11">
       <td valign="top" class="td11">
-
        <p class="p3"><span class="s1">1.58E-11</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td12">
 
         <p class="p3"><span class="s1">1.6E-03</span></p>
         <p class="p3"><span class="s1">1.6E-03</span></p>
       </td>
       </td>
-
       <td valign="top" class="td13">
+
       <td valign="top" class="td12">
         <p class="p3"><span class="s1">2.5E-05</span></p>
         <p class="p3"><span class="s1">2.5E-05</span></p>
       </td>
       </td>
-
       <td valign="top" class="td9">
+
       <td valign="top" class="td13">
         <p class="p3"><span class="s1">5.0E-03</span></p>
         <p class="p3"><span class="s1">5.0E-03</span></p>
       </td>
       </td>
       <td valign="top" class="td14">
       <td valign="top" class="td14">
-
         <p class="p3"><span class="s1">2.5E-05</span></p>
+
         <p class="p3"><span class="s1">1.6E-01</span></p>
 +
      </td>
 +
      <td valign="top" class="td14">
 +
        <p class="p3"><span class="s1">3.8E-09</span></p>
       </td>
       </td>
       <td valign="top" class="td15">
       <td valign="top" class="td15">
-
         <p class="p3"><span class="s1"><b>5.0E-03</b></span></p>
+
         <p class="p3"><span class="s1">2.5E-05</span></p>
       </td>
       </td>
       <td valign="top" class="td16">
       <td valign="top" class="td16">
-
         <p class="p3"><span class="s1">6.3E-05</span></p>
+
         <p class="p3"><span class="s1"><b>5.0E-03</b></span></p>
 +
      </td>
 +
      <td valign="top" class="td17">
 +
        <p class="p3"><span class="s1">7.7E-07</span></p>
 +
      </td>
 +
      <td valign="top" class="td18">
 +
        <p class="p3"><span class="s1">1.5E-02</span></p>
       </td>
       </td>
     </tr>
     </tr>
   </tbody>
   </tbody>
</table>
</table>
 +
<p class="p1"><br></p>
<p class="p1"><br></p>

Revision as of 07:54, 17 July 2014

[DCM] calibration for solution-vapour equilibration

Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a real binary solution/ideal gas, based on literature solubility data, confirming negligible [DCM] deviation (~ orders of magnitude smaller than the uncertainty in [DCM]) and that :

V(DCM) added (± 5E-4) /ml

n(DCM)(l) unadjusted (± 8E-6) /mol

[DCM] unadjusted (± 2E-3) /M

p(DCM) 

(± 4E-2) /kPa

n(DCM)(g) 

(± 4E-7) /mol

n(DCM)(l) (± 9E-6) adjusted /mol

[DCM] adjusted (± 2E-3) /M

Δ[DCM] /M

[DCM] % deviation

(30°C, 5mM)

6.4E-03

1.0E-04

2.0E-02

6.2E-01

1.6E-08

1.0E-04

2.0E-02

3.1E-06

1.6E-02

(30°C, 10mM)

3.2E-03

5.0E-05

1.0E-02

3.1E-01

7.9E-09

5.0E-05

1.0E-02

1.6E-06

1.6E-02

(30°C, 20mM)

1.6E-03

2.5E-05

5.0E-03

1.6E-01

3.9E-09

2.5E-05

5.0E-03

7.9E-07

1.6E-02











(37°C, 5mM)

6.4E-03

1.0E-04

2.0E-02

6.2E-01

1.5E-08

1.0E-04

2.0E-02

3.1E-06

1.5E-02

(37°C, 10mM)

3.2E-03

5.0E-05

1.0E-02

3.1E-01

7.7E-09

5.0E-05

1.0E-02

1.5E-06

1.5E-02

(37°C, 20mM)

1.6E-03

2.5E-05

5.0E-03

1.6E-01

3.8E-09

2.5E-05

5.0E-03

7.7E-07

1.5E-02


more detailed system modelling is therefore unnecessary.

Retrieved from "http://2014.igem.org/Team:Oxford/modellingA"