Revision as of 22:22, 17 October 2014

Mutation·LABNOTE

May: amplify biobricks

June: clone the WT DNAQ ,recA,umuC,umuD from E.coli

July: perform the point-mutation and truncation of the four genes

August: add prefix and suffix to the genes

&nbsp&nbsp&nbsp&nbspAdd RBS to the genes

September: Construct the polycistron plasmid (DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;

&nbsp&nbsp&nbsp&nbspDNAQ926+umuD’+umuC+recA730;) <p>&nbsp&nbsp&nbsp&nbspAdd promoter to construct the expression vector <p> Test the mutation rate

October: adapt the testing method and test the mutation rates.

&nbsp&nbsp&nbsp&nbspPrepare the biobrick for submission

@@ Line 4: / Line 4: @@
 <p>June: clone the WT DNAQ ,recA,umuC,umuD from E.coli </p>
 <p>July: perform the point-mutation and truncation of the four genes</p>
-<p>August: add prefix and suffix to the genes
+<p>August: add prefix and suffix to the genes</p>
-       Add RBS to the genes</p>
+<p>&nbsp&nbsp&nbsp&nbspAdd RBS to the genes</p>
-<p>September: Construct the polycistron plasmid (DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;DNAQ926+umuD’+umuC+recA730;)
+<p>September: Construct the polycistron plasmid (DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;</p>
-          Add promoter to construct the expression vector
+<p>&nbsp&nbsp&nbsp&nbspDNAQ926+umuD’+umuC+recA730;)
-          Test the mutation rate</p>
+<p>&nbsp&nbsp&nbsp&nbspAdd promoter to construct the expression vector
-<p>October: adapt the testing method and test the mutation rates
+<p>          Test the mutation rate</p>
-        Prepare the biobrick for submission </p>
+<p>October: adapt the testing method and test the mutation rates.</p>
+<p>&nbsp&nbsp&nbsp&nbspPrepare the biobrick for submission </p>
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