"
Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014
From 2014.igem.org
(Difference between revisions)
| (5 intermediate revisions not shown) | |||
| Line 2: | Line 2: | ||
<div class="cd-timeline-block"> | <div class="cd-timeline-block"> | ||
</html> | </html> | ||
| - | {{:Team:Evry/Notebook/CellCharacterization/ | + | {{:Team:Evry/Notebook/CellCharacterization/Transformation/TopImage}} |
<html> | <html> | ||
<div class="cd-timeline-content"> | <div class="cd-timeline-content"> | ||
| + | |||
<p align="justify"> | <p align="justify"> | ||
| - | <u> <b> Amplification of plasmids pBBR1MCS and pRhokHI-2 </b> </u> <br> | + | |
| + | <u> <b> <big><FONT COLOR=#003333>Amplification of plasmids pBBR1MCS and pRhokHI-2 in E.Coli Bl21</font></big></b> </u> <br> | ||
Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology) | Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology) | ||
| Line 14: | Line 16: | ||
<img src="https://static.igem.org/mediawiki/2014/f/f2/Plamids.jpg" alt="plasmidS"/> | <img src="https://static.igem.org/mediawiki/2014/f/f2/Plamids.jpg" alt="plasmidS"/> | ||
</div> | </div> | ||
| - | <br> | + | <br><p> |
| - | Bowth were transfered into Bl21 electro-competent cells by electroporation (two replicate) | + | Bowth were transfered into Bl21 electro-competent cells by electroporation (two replicate)(1µL of plasmid for 40µL of cells, 1800V). <br> After a liquid culture of three hours, cells were plated on LB 1X + Cam (1:1000) and let in incubator overnight.</p> |
Latest revision as of 22:21, 17 October 2014
Amplification of plasmids pBBR1MCS and pRhokHI-2 in E.Coli Bl21
Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)
Bowth were transfered into Bl21 electro-competent cells by electroporation (two replicate)(1µL of plasmid for 40µL of cells, 1800V).
After a liquid culture of three hours, cells were plated on LB 1X + Cam (1:1000) and let in incubator overnight.
