Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014

From 2014.igem.org

(Difference between revisions)

Latest revision as of 22:21, 17 October 2014

Amplification of plasmids pBBR1MCS and pRhokHI-2 in E.Coli Bl21
Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)

Bowth were transfered into Bl21 electro-competent cells by electroporation (two replicate)(1µL of plasmid for 40µL of cells, 1800V).
After a liquid culture of three hours, cells were plated on LB 1X + Cam (1:1000) and let in incubator overnight.

Aug 23

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+{{:Team:Evry/Notebook/CellCharacterization/Transformation/TopImage}}
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-<img src=""  alt="pBBR1MCS"/>
-<img src=""  alt="phokH1-2"/>
+<u> <b> <big><FONT COLOR=#003333>Amplification of plasmids pBBR1MCS and pRhokHI-2 in E.Coli Bl21</font></big></b> </u> <br>
+Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)
+<br>
+<div align="center">
+<img src="https://static.igem.org/mediawiki/2014/f/f2/Plamids.jpg"  alt="plasmidS"/>
+</div>
+<br><p>
+Bowth were transfered into Bl21 electro-competent cells by electroporation (two replicate)(1µL of plasmid for 40µL of cells, 1800V). <br> After a liquid culture of three hours, cells were plated on LB 1X + Cam (1:1000) and let in incubator overnight.</p>