Team:WashU StLouis/Notebook/August

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<h1> <center><b>August</b> </center></h1>
<h1> <center><b>August</b> </center></h1>
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<div style="text-align: center;">
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<h3><a href="https://2014.igem.org/Team:WashU_StLouis/Notebook">Notebook</a>
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&nbsp;&nbsp;&nbsp; |&nbsp;&nbsp; &nbsp; <a
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href="https://2014.igem.org/Team:WashU_StLouis/Notebook/June">June</a>
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&nbsp;&nbsp;&nbsp; |&nbsp;&nbsp; &nbsp; <a
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href="https://2014.igem.org/Team:WashU_StLouis/Notebook/July">July</a>
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&nbsp;&nbsp;&nbsp; |&nbsp;&nbsp; &nbsp; <a href="https://2014.igem.org/Team:WashU_StLouis/Notebook/September">School Year</a></h3>
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<center> <img style="border: 0px solid ; width: 100%" alt="August Calendar" src="https://static.igem.org/mediawiki/2014/9/9c/WashU_August_Calendar.png"> </center> <br><br>
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<td style="vertical-align: top;"><img
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<div style="text-align: center;"><span style="font-weight: bold;">Week
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Ten- August 4-10</span><br>
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</div>
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Continued Acetylene Reduction Assay for all strains and controls. <br>
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Ran light induction experiment and troubleshoot data based on results.
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Started design of a new positive control to more accurately find out
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what is wrong with our system. <br>
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Had our final Worldly Wednesday at Blueberry Hill before people started
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leaving campus before school starts.<br>
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Website rearranged formatting of the basic page. Change color scheme and background. <br>
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Jeffrey left the team for the summer to go abroad.</td>
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<td style="vertical-align: top; text-align: center;"><img
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style="width: 300px; height: 300px;" alt="Week 11 Picture"
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src="https://static.igem.org/mediawiki/2014/e/ec/WashU_Week_11.JPG"><br>
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      <span style="font-weight: bold;">Week Eleven- August 11-17</span><br><div style="text-align: left;">
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<p> insert data here </p>
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Presented our preliminary results to Pakrasi Group and all of the advisors. <br>
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Ben started and tested new positive control and found that it did not
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fluoresce as expected and suspect a faulty RBS. Started design of
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primers to modify the RBS of the system.</div></td>
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<td style="vertical-align: top; text-align: center;"><img
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style="width: 300px; height: 405px;" alt="School year begins"
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src="https://static.igem.org/mediawiki/2014/2/23/WashU_Week_onwards.jpg"><br>
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<td style="vertical-align: top; text-align: center;"><span style="font-weight: bold;">Week Twelve- August 25 - 31</span><br><div style="text-align: left;">
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School year started. <br>Rebstock group continued to use acetylene reduction assay to test nitrogen fixation activity of JM109 and WM1788 under known optimal conditions.<br>
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Meanwhile Rebstock group started to plan specific protocol and design for BioBricking the <i>nif</i> plasmid.<br>
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Ben starting cloning project to test whether switching the rbs to a comparable one would fix the EYFP expression in the light plasmids. He sent the clones in for sequencing.</i>
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Ben also worked on the title and abstract for the iGEM September 1st deadline.
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Latest revision as of 22:15, 17 October 2014



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