Team:TCU Taiwan/Attributions

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                 <li>Transformation clone RFP/pBluescript SK(-) to DH5α.<br>
                 <li>Transformation clone RFP/pBluescript SK(-) to DH5α.<br>
                 <li>Extract plasmid clone RFP/pBluescript SK(-).</li></font></td>
                 <li>Extract plasmid clone RFP/pBluescript SK(-).</li></font></td>
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                 <li> Release titer.(Releasing titer of phagemid which is <em>E.coli</em> infected by helper phage)<br>
                 <li> Release titer.(Releasing titer of phagemid which is <em>E.coli</em> infected by helper phage)<br>
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               <td width="50%" height="30px">&nbsp;</td>
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               <td>&nbsp;</td>
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    <td><p align="center">&nbsp;</p>
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      <p align="center"><font size="7" face="Verdana" color="#D92332">Acknowledgements</font></p></td>
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    <td valign="top"><p align="center">&nbsp;</p>
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      <p><font size="5" face="Verdana" color="#333">We have taken efforts in this project. However, it would not have been possible without the kind support and help of many individuals and organizations. We would like to extend our sincere thanks to all of them.</font></p></td>
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Latest revision as of 22:08, 17 October 2014


 
Attributions
  
We would like to thank all the people who came to our progress presentations and gave useful feedback.
 
Instructors support
 
Lin,Guang-Huey

Professor Lin teaches knowledge about microbiology for TCU iGEM team each year, and she also guides our experimental design. Besides, she provides her lab for us to working on our experiment.

  
Lin,Nien-Tsung

Prof. Lin, a gracious teacher, is the guiders of our experiment on M13 phage display. She provides us a lot knowledge about M13 phage and DNA cloning.
     
Ching,Yung-Hao

Prof. Ching is one of the leader teachers of our team. She is a kind teacher who always encourages us, and she also gives us much advice and direction on safety concerns.

 
Woon,Peng-Yeong

Prof. Woon is one of the leader teachers for this year. He helps us the basic concept of iGEM and reminds us many iGEM rules or requests we nearly missed. He is a lively professor.
   
Experiment
 
Bobo, Regina, Denise, Eating
 
Contributions
 
  • Transformation ( Cas9 in pSB1C3 into DH5α)
  • Extract plasmid with pSB1C3 from DH5α.
  • Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.
  • Use Enzyme digestion (PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
  • Purification of RFP sequence from pSB1C3
  • Prepare Cm. plate、LB. plate.
  • Ligation of RFP and pBluescript SK(-).
  • Transformation clone RFP/pBluescript SK(-) to DH5α.
  • Extract plasmid clone RFP/pBluescript SK(-).
    		•

    Experiment Design

    Ted and Bobo

    Design gRNA for AmpR.

    Biobricks Design

    Ted

     Design the gRNA for different resistance gene.

       
    Modeling
     
    House, Bobo, Chao-Di Chang(National Chiao Tung University)
     
    Contributions
     
  • Infectional efficiency ratio of M13KO7.
  • Release titer.(Releasing titer of phagemid which is E.coli infected by helper phage)
    		•

    Special Thanks

    Modeling Advisor

    Chang,Chao-Di


    Thanks for Chang,Chao-Di strong support on our modeling.
       
    Human Practice
     
    Eating
     
    Contributions
     
  • Held the 2014 Synthetic Biology information meeting of local senior high school and Tzu Chi University as a chief convener.
  • Contact with National Chiao Tung University for cooperation.
    		•
       
    Wiki
     
    House
     
    Contributions
     
  • Construct the wiki page.
  • Coding,coding and coding the wiki page.
  • Study all about Synthetic Biology from wetlab to drylab.
    		•
     
    And last but not least
    Art Design

    Denise, Regina, Eating, House

    Design of poster and PPT.
    Design of team uniform.

    		 
    Photo & Video

    Sharon, Eating

     Take photo and video.
    Arrange all photos.

    Administeration

    Eating

    Apply for the allowances.
    Arrange all files.

    		  
       
    Video support
     
    Wang, Yiru and Chen, Jiawei
     
    we should be obliged to them for their assistance in this matter.
     

    Wang, Yi-ru

    Chen, Jia-wei
       
    Special Thanks
     
    Our partner : NCTU_Formosa
     
  • We are extremely appreciative of NCTU_Formosa that helped and support us.
  • The model1 courtesy of Chao-Di Chang.
    		•
       
    Sponsors
     
               
    General support
     
    Master program in Microbiology and Immunology
     
    Department of Medical Informatics
     
    Department of Molecular Biology and Human Genetics
       

     

    Acknowledgements

     

    We have taken efforts in this project. However, it would not have been possible without the kind support and help of many individuals and organizations. We would like to extend our sincere thanks to all of them.
    ^


        
    Team Members Project Parts Human Pratics Modeling Safety Notebook Attributions

    Lost the way? Use it to help you if you're lost.

    ^

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