Team:UFAM Brazil/Biosensor
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<p align="center"><img src="https://static.igem.org/mediawiki/2014/5/57/UFAM_BRAZIL_2014_biosen2.png" width="600"></p> | <p align="center"><img src="https://static.igem.org/mediawiki/2014/5/57/UFAM_BRAZIL_2014_biosen2.png" width="600"></p> | ||
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<h3 align="center">Experiments and Results</h3> | <h3 align="center">Experiments and Results</h3> | ||
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The graph represented on Figure 1 shows the Optical Density of transformed DH5α with BBa_K1355002 in different Hg concentrations in function of time: | The graph represented on Figure 1 shows the Optical Density of transformed DH5α with BBa_K1355002 in different Hg concentrations in function of time: | ||
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<p align="center"><img src="https://static.igem.org/mediawiki/2014/1/1f/UFAM_BRAZIL_2014_biosen3.png" width="600"></p> | <p align="center"><img src="https://static.igem.org/mediawiki/2014/1/1f/UFAM_BRAZIL_2014_biosen3.png" width="600"></p> | ||
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<p align="center">Figure 2. GFP fluorescence intensity in the four given times, at mercury chloride concentrations of 0 µg/ml, 0.01 µg/ml, 0.02 µg/ml, 0.1 µg/ml, 0.2 µg/ml, and 1 µg/ml.</p> | <p align="center">Figure 2. GFP fluorescence intensity in the four given times, at mercury chloride concentrations of 0 µg/ml, 0.01 µg/ml, 0.02 µg/ml, 0.1 µg/ml, 0.2 µg/ml, and 1 µg/ml.</p> | ||
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<p>Farias, L. A., Fávaro, D. I., Pessoa, A., Aguiar, J. P., & Yuyama, L. K. (2012). Mercury and methylmercury concentration assessment in children's hair from Manaus, Amazonas state, Brazil. Acta Amazonica, 42(2), 279-286.</p> | <p>Farias, L. A., Fávaro, D. I., Pessoa, A., Aguiar, J. P., & Yuyama, L. K. (2012). Mercury and methylmercury concentration assessment in children's hair from Manaus, Amazonas state, Brazil. Acta Amazonica, 42(2), 279-286.</p> | ||
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<p>Fillion, M., Philibert, A., Mertens, F., Lemire, M., Passos, C. J. S., Frenette, B., ... & Mergler, D. (2011). Neurotoxic sequelae of mercury exposure: an intervention and follow-up study in the Brazilian Amazon. Ecohealth, 8(2), 210-222.</p> | <p>Fillion, M., Philibert, A., Mertens, F., Lemire, M., Passos, C. J. S., Frenette, B., ... & Mergler, D. (2011). Neurotoxic sequelae of mercury exposure: an intervention and follow-up study in the Brazilian Amazon. Ecohealth, 8(2), 210-222.</p> | ||
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<p>Grotto, D., Valentini, J., Fillion, M., Passos, C. J. S., Garcia, S. C., Mergler, D., & Barbosa Jr, F. (2010). Mercury exposure and oxidative stress in communities of the Brazilian Amazon. Science of the Total Environment, 408(4), 806-811.</p> | <p>Grotto, D., Valentini, J., Fillion, M., Passos, C. J. S., Garcia, S. C., Mergler, D., & Barbosa Jr, F. (2010). Mercury exposure and oxidative stress in communities of the Brazilian Amazon. Science of the Total Environment, 408(4), 806-811.</p> | ||
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<p>Hakkila, K., Maksimow, M., Karp, M., & Virta, M. (2002). Reporter Genes lucFF, luxCDABE, gfp, and dsred Have Different Characteristics in Whole-Cell Bacterial Sensors. Analytical biochemistry, 301(2), 235-242.</p> | <p>Hakkila, K., Maksimow, M., Karp, M., & Virta, M. (2002). Reporter Genes lucFF, luxCDABE, gfp, and dsred Have Different Characteristics in Whole-Cell Bacterial Sensors. Analytical biochemistry, 301(2), 235-242.</p> | ||
+ | <p>Kuncova, G., Pazlarova, J., Hlavata, A., Ripp, S., & Sayler, G. S. (2011). Bioluminescent bioreporters Pseudomonas putida TVA8 as a detector of water pollution. Operational conditions and selectivity of free cells sensor. Ecological Indicators, 11(3), 882-887.</p> | ||
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Latest revision as of 22:06, 17 October 2014