Team:British Columbia/Notebook/Protocol

From 2014.igem.org

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     Instruction for colony PCR and regular PCR
     Instruction for colony PCR and regular PCR
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     <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/gelverificaation">Gel Verification</a> </font> </b>
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    Make a gel and conduct gel elecrophoresis
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Revision as of 21:44, 17 October 2014

2014 UBC iGEM

Protocols

  • PCR
    • Instruction for colony PCR and regular PCR
  • Gel Verification
    • Make a gel and conduct gel elecrophoresis
===[[Team:British_Columbia/Notebook/Protocols/PCR|PCR]]=== Instruction for colony PCR and regular PCR ===[[Team:British_Columbia/Notebook/Protocols/gelverification|Gel Verification]]=== Make a gel and conduct gel elecrophoresis ===[[Team:British_Columbia/Notebook/Protocols/Biobrickdigest|BioBrick Restriction Digest]]=== Restriction digest with BioBrick plasmid an inserts ===[[Team:British_Columbia/Notebook/Protocols/ligation|BioBrick Ligation]]=== Ligate Biobrick insert to vector ===[[Team:British_Columbia/Notebook/Protocols/plates|Making Plates]]=== Make LB plates with antibiotics ===[[Team:British_Columbia/Notebook/Protocols/media|Liquid media]]=== Make liquid LB media ===[[Team:British_Columbia/Notebook/Protocols/compcells|Competent cells]]=== Preparation of chemically competent cells ===[[Team:British_Columbia/Notebook/Protocols/transformation|Transformation]]=== Transformation of plasmi DNA into chemically competent cells ===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]=== Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides ===[[Team:British_Columbia/Notebook/Protocols/AnnealedOligonucleotides|Assembling Parts from Oligonucleotides]]=== Assemble short parts from annealed oligonucleotides ===[[Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis|(Multi) Site Directed Mutagenesis]]=== Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations. ===[[Team:British_Columbia/Notebook/Protocols/T4PNK|Oligonucleotide Phosphorylation]]=== Phosphorylate oligonucleotides prior to ligation ===[[Team:British_Columbia/Notebook/Protocols/ElectrocompCells|Electrocompetent Cell Production]]=== Make electrocompetent cells ===[[Team:British_Columbia/Notebook/Protocols/CombinatorialLibraries|Make Combinatorial Part Libraries]]=== Make combinatorial part libraries from compatible parts

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