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Team:Groningen/Template/MODULE/Notebook/characterisation/week2
From 2014.igem.org
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| - | Obtaining the NisA (<a href="http://parts.igem.org/Part:BBa_K1365000">BBa_K1365000</a>) and Superfolded GFP (<a href="http://parts.igem.org/Part:BBa_K1365020">BBa_K1365020</a>) BioBricks in larger concentration for further use | + | Obtaining the NisA (<a href="http://parts.igem.org/Part:BBa_K1365000">BBa_K1365000</a>) and the Superfolded GFP gene (<a href="http://parts.igem.org/Part:BBa_K1365020">BBa_K1365020</a>) BioBricks in larger concentration for further use |
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| - | <div class="item">Transformed both, pSB1C3 containing the NisA BioBrick and pSB1C3 with the Superfolded GFP Biobrick, to two different <i>E. coli</i> DH5α strains</div> | + | <div class="item">Transformed both, pSB1C3 containing the NisA BioBrick and pSB1C3 with the Superfolded GFP gene Biobrick, to two different <i>E. coli</i> DH5α strains</div> |
<div class="item">The transformations were checked on gel, and the desired bands were observed, therefore the transformation was most likely successful</div> | <div class="item">The transformations were checked on gel, and the desired bands were observed, therefore the transformation was most likely successful</div> | ||
<div class="item">The plasmids with the desired BioBricks were isolated out of <i>E. coli</i> DH5α, and glycerol stocks were made of the two strains containing the previous named parts</div> | <div class="item">The plasmids with the desired BioBricks were isolated out of <i>E. coli</i> DH5α, and glycerol stocks were made of the two strains containing the previous named parts</div> | ||
Revision as of 21:31, 17 October 2014
September 8 - September 14
Obtaining the NisA (BBa_K1365000) and the Superfolded GFP gene (BBa_K1365020) BioBricks in larger concentration for further use
Transformed both, pSB1C3 containing the NisA BioBrick and pSB1C3 with the Superfolded GFP gene Biobrick, to two different E. coli DH5α strains
The transformations were checked on gel, and the desired bands were observed, therefore the transformation was most likely successful
The plasmids with the desired BioBricks were isolated out of E. coli DH5α, and glycerol stocks were made of the two strains containing the previous named parts
