Team:Gaston Day School/Parts
From 2014.igem.org
(Difference between revisions)
(15 intermediate revisions not shown) | |||
Line 254: | Line 254: | ||
<div class="row"> | <div class="row"> | ||
- | < | + | <h1 id="mainhead" style="color:#072751" class="show" align="center">2014 GDS iGEM Parts Page</p> |
+ | <h2 style="color:#072751">>Parts</h2> | ||
</div> | </div> | ||
Line 301: | Line 302: | ||
<div class="col-1-2"> | <div class="col-1-2"> | ||
<h2 id="head">Cd Detector</h2> | <h2 id="head">Cd Detector</h2> | ||
- | < | + | <div class="show" id="pad"> |
- | K1320000 | + | <a href="http://parts.igem.org/Part:BBa_K1320000"> |
- | </ | + | K1320000 |
+ | </a> | ||
+ | <br> | ||
+ | <br>In 2012 our team created a cadmium detector part K824008. We also created several other heavy metal detectors, but since our team was unable to gain access to other heavy metals due to our BS1 status, and cadmium was quickly becoming a local issue, we decided to focus on improving our cadmium detector. This first detector was sensitive to 10 mM of cadmium. This year, with the addition of sensitivity tuners, we improved the sensitivity four-fold. We can now detect 2.5 mM of cadmium, which is a much more realistic and useful concentration to find. At 2.5 mM of cadmium, the presence is not necessarily apparent in water, but is still above safe levels and the federal limit. | ||
+ | <br> | ||
+ | <br>2012 Cd Detector Results | ||
+ | <br><img src="https://static.igem.org/mediawiki/2014/2/21/2012_Cd_graph.jpg" alt="6.11.14iso.png" style="width:100%"> | ||
+ | <br> | ||
+ | <br>2014 Cd Detector Results | ||
+ | <br><img src="https://static.igem.org/mediawiki/2014/d/db/Cadmium_graph.jpg" alt="6.11.14iso.png" style="width:100%"> | ||
+ | |||
+ | |||
+ | </div> | ||
</div> | </div> | ||
<div class="col-1-2"> | <div class="col-1-2"> | ||
<h2 id="head">GlmY</h2> | <h2 id="head">GlmY</h2> | ||
- | < | + | <div class="show" id="pad"> |
- | + | <a href="http://parts.igem.org/Part:BBa_K1320100"> | |
- | + | K1320100 | |
- | + | </a> | |
- | + | <br> | |
- | + | <br> Gel showing our working GlmY construct. Wells are as follows: | |
- | + | <br>1.Ladder | |
- | + | <br>2.Control | |
- | + | <br>3.GlmY | |
- | + | <br>4.GlmY | |
- | + | <br><img src="https://static.igem.org/mediawiki/2014/thumb/3/39/Working_GlmY_Gel.jpg/336px-Working_GlmY_Gel.jpg" alt="6.11.14iso.png" style="width:100%"> | |
- | + | </div> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | </ | + | |
</div> | </div> | ||
</div> | </div> |
Latest revision as of 21:23, 17 October 2014
2014 GDS iGEM Parts Page
>Parts
Cd Detector
K1320000
In 2012 our team created a cadmium detector part K824008. We also created several other heavy metal detectors, but since our team was unable to gain access to other heavy metals due to our BS1 status, and cadmium was quickly becoming a local issue, we decided to focus on improving our cadmium detector. This first detector was sensitive to 10 mM of cadmium. This year, with the addition of sensitivity tuners, we improved the sensitivity four-fold. We can now detect 2.5 mM of cadmium, which is a much more realistic and useful concentration to find. At 2.5 mM of cadmium, the presence is not necessarily apparent in water, but is still above safe levels and the federal limit.
2012 Cd Detector Results
2014 Cd Detector Results
In 2012 our team created a cadmium detector part K824008. We also created several other heavy metal detectors, but since our team was unable to gain access to other heavy metals due to our BS1 status, and cadmium was quickly becoming a local issue, we decided to focus on improving our cadmium detector. This first detector was sensitive to 10 mM of cadmium. This year, with the addition of sensitivity tuners, we improved the sensitivity four-fold. We can now detect 2.5 mM of cadmium, which is a much more realistic and useful concentration to find. At 2.5 mM of cadmium, the presence is not necessarily apparent in water, but is still above safe levels and the federal limit.
2012 Cd Detector Results
2014 Cd Detector Results
GlmY
K1320100
Gel showing our working GlmY construct. Wells are as follows:
1.Ladder
2.Control
3.GlmY
4.GlmY
Gel showing our working GlmY construct. Wells are as follows:
1.Ladder
2.Control
3.GlmY
4.GlmY