Team:Goettingen/protocol

From 2014.igem.org

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<div id="protocolImg"><img src="https://static.igem.org/mediawiki/2014/d/d7/Goettingen_slider3.png" width="1002" height="360" /></div>
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        <div id="protocolImg" ><img src="https://static.igem.org/mediawiki/2014/c/cb/Goettingen-Slider3_reduced-banner.jpg" width="1002" height="360" /></div>
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        <h2 id="OverviewTitle">Overview</h2>
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<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR">PCR Methods</a></h4>
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        <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR">Polymerase chain reaction(PCR) Methods</a></h4>
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<ul>
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<ul>
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<li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Pfus_PCR">Pfusion PCR</a></li>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Pfus_PCR"><i>PfuS</i> PCR</a></li>
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<li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Gra_PCR">Gradient PCR</a></li>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Fusi_PCR">Fusion PCR</a></li>
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</ul>
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            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#E_col"><i>E.coli</i> colony PCR</a></li>
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            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Y_col">Yeast colony PCR</a></li>
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</ul>
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<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA">DNA Assessment</a></h4>
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            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#Gel_Ext">Gel Extraction</a></li>
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<li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#Gel_Ele">DNA Gel Electrophoresis</a></li>
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            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#Seq">Pretreatment of DNA for Sequencing</a></li>
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            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#Flu">Fluorescence Assay</a></li>
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            </ul>
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        </div>
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             <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4>
             <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4>
             <ul>
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            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#rest_DNA">Restriction of DNA</a></li>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#liga_DNA">Ligation of DNA fragments</a></li>
             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#BP">BP recombination reaction</a></li>
             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#BP">BP recombination reaction</a></li>
             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LR">LR recombination reaction</a></li>
             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LR">LR recombination reaction</a></li>
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            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#seam_clone">SEAMLESS Cloning</a></li>
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            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LB">Peptide Library construction</a></li>
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             </ul>
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             <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran">Plasmid Transformation</a></h4>
             <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran">Plasmid Transformation</a></h4>
             <ul>
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             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#"><i>E.coli</i> competence cells</a></li>
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             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_comp"><i>E.coli</i> competent cells</a></li>
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            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_plas">Plasmid isolation (<i>E.coli</i>)</a></li>
             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_tran"><i>E.coil</i> transformation</a></li>
             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_tran"><i>E.coil</i> transformation</a></li>
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            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_iso">Plasmid isolation (Yeast)</a></li>
             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_tran">Yeast transformation</a></li>
             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_tran">Yeast transformation</a></li>
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             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li>
             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li>
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            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y_col">Yeast colony PCR</a></li>
 
             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li>
             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li>
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             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_Scr">Yeast-Two-Hybrid Screening</a></li>
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             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_robot">Yeast-Two-Hybrid Assay with Robot</a></li>
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             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_bw">Y2H blue/white selection</a></li>
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             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_screen">Yeast-Two-Hybrid Screening</a></li>
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             </ul>
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            <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Peptide">Peptide library</a></h4>
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        <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_protein">Protein Assessment</a></li>
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            <ul>
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            <li><a href="#"></a></li>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#SDS">SDS PAGE</a></li>
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            </ul>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Coo">Coomassie Staining</a></li>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#P_Exp">Protein Expression</a></li>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Puri">Protein Purification</a></li>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Dialysis">Protein Dialysis </a></li>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Bradford">Bradford Assay</a></li>
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</ul>
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      </div>
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        <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo"><i>In vivo</i> tests</a></li>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Prod">Production of spore suspension</a></li>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Culti_A">Cell cultivation</a></li>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#immu">Immunofluorescence Staining </a></li>
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    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Calc">Calcofluor staining</a></li>
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</ul>
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      </div>
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</div>
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Latest revision as of 21:15, 17 October 2014