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Team:Goettingen/protocol
From 2014.igem.org
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<div id="protocolImg" ><img src="https://static.igem.org/mediawiki/2014/c/cb/Goettingen-Slider3_reduced-banner.jpg" width="1002" height="360" /></div> | <div id="protocolImg" ><img src="https://static.igem.org/mediawiki/2014/c/cb/Goettingen-Slider3_reduced-banner.jpg" width="1002" height="360" /></div> | ||
| + | <h2 id="OverviewTitle">Overview</h2> | ||
<div class="pro_sub"> | <div class="pro_sub"> | ||
| - | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR">PCR Methods</a></h4> | + | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR">Polymerase chain reaction(PCR) Methods</a></h4> |
<ul> | <ul> | ||
| - | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Pfus_PCR"><i>PfuS</i> PCR</a></li> | |
| - | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Fusi_PCR">Fusion PCR</a></li> | |
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#E_col"><i>E.coli</i> colony PCR</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Y_col">Yeast colony PCR</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="pro_sub"> | <div class="pro_sub"> | ||
<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4> | ||
<ul> | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#rest_DNA">Restriction of DNA</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#liga_DNA">Ligation of DNA fragments</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#BP">BP recombination reaction</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#BP">BP recombination reaction</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LR">LR recombination reaction</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LR">LR recombination reaction</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#seam_clone">SEAMLESS Cloning</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LB">Peptide Library construction</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran">Plasmid Transformation</a></h4> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran">Plasmid Transformation</a></h4> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#"><i>E.coli</i> | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_comp"><i>E.coli</i> competent cells</a></li> |
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_plas">Plasmid isolation (<i>E.coli</i>)</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_tran"><i>E.coil</i> transformation</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_tran"><i>E.coil</i> transformation</a></li> | ||
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_iso">Plasmid isolation</a></li> | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_iso">Plasmid isolation (Yeast)</a></li> |
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_tran">Yeast transformation</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_tran">Yeast transformation</a></li> | ||
</ul> | </ul> | ||
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<ul> | <ul> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li> | ||
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<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li> | ||
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony# | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_robot">Yeast-Two-Hybrid Assay with Robot</a></li> |
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony# | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_screen">Yeast-Two-Hybrid Screening</a></li> |
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</ul> | </ul> | ||
</div> | </div> | ||
| + | <div class="pro_sub"> | ||
| + | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_protein">Protein Assessment</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#SDS">SDS PAGE</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Coo">Coomassie Staining</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#P_Exp">Protein Expression</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Puri">Protein Purification</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Dialysis">Protein Dialysis </a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Bradford">Bradford Assay</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | <div class="pro_sub"> | ||
| + | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo"><i>In vivo</i> tests</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Prod">Production of spore suspension</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Culti_A">Cell cultivation</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#immu">Immunofluorescence Staining </a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Calc">Calcofluor staining</a></li> | ||
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| + | </ul> | ||
| + | </div> | ||
</div> | </div> | ||
Latest revision as of 21:15, 17 October 2014
