Team:ETH Zurich/expresults/qs/tab-prhl

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Revision as of 20:57, 17 October 2014

**Table 3** Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])
In this set of experiments the promoter pRhl (ref) was tested for potential crosstalk. In the top left position we observe the induction of pRhl (ref)by C4-HSL bound to the regulator RhlR(ref). The switching behaviour was observed at a C4-HSL concentration of 1 μM. In the case of 3OC12-HSL binding the RhlR (ref)regulator and subsequently the promoter pRhl (ref)insignificant crosstalk has been observed. Severe crosstalk was observed in the case of 3OC6-HSL binding the RhlR (reg)regulator followed by induction of pRhl(reg). The transition occurred at a concentration of the inducer molecule of 1 μM but compared to the reference curve a lower value of fluorescence per OD was observed (1000 a.u.). Another case of crosstalk with the pRhl (ref) was detected with 3OC12-HSL binding to the corresponding LasR (ref)regulator followed by inducing the promoter pRhl(ref). Here switching occurred at a concentration 1 nM of 3OC12-HSL and reached fluorescence per OD of 750 a.u.. This is approximately 0.5 fold the value of the fluorescence per OD shown by the reference curve indicated in green.

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 |+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])
-|colspan="4" style='font-size:10pt';text-align:left|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM.
+|colspan="4" style='font-size:10pt';text-align:left|In this set of experiments the promoter pRhl (ref) was tested for potential crosstalk. In the top left position we observe the induction of pRhl (ref)by C4-HSL bound to the regulator RhlR(ref). The switching behaviour was observed at a C4-HSL concentration of 1 μM.
-In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.
+In the case of 3OC12-HSL binding the RhlR (ref)regulator and subsequently the promoter pRhl (ref)insignificant crosstalk has been observed.
-Severe crosstalk was detected in the case of 3OC6-HSL binding the RhlR regulator followed by induction of pRhl. The transition occurred at a concentration of the inducer molecule of 10<sup>3</sup> nM but compared to the reference curve a lower (1000 AU) value of fluorescence was observed. The second case of crosstalk with the pRhl was detected with 3OC12-HSL binding to the corresponding LasR regulator followed by inducing the promoter pRhl. There the switching occurred at a concentration 1nM of 3OC12-HSL reaching a fluorescence of 750 AU. This is approximately 0.5 fold the value of the fluorescence shown by the reference curve in green.
+Severe crosstalk was observed in the case of 3OC6-HSL binding the RhlR (reg)regulator followed by induction of pRhl(reg). The transition occurred at a concentration of the inducer molecule of 1 μM but compared to the reference curve a lower  value of fluorescence per OD was observed (1000 a.u.). Another case of crosstalk with the pRhl (ref) was detected with 3OC12-HSL binding to the corresponding LasR (ref)regulator followed by inducing the promoter pRhl(ref). Here switching occurred at a concentration 1 nM of 3OC12-HSL and reached fluorescence per OD of 750 a.u.. This is approximately 0.5 fold the value of the fluorescence per OD shown by the reference curve indicated in green.
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