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Team:TU Delft-Leiden/Project/Notebook
From 2014.igem.org
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<p>General work </p> | <p>General work </p> | ||
</a></p> | </a></p> | ||
| - | <p><a href="https:// | + | <p><a href="https://static.igem.org/mediawiki/2014/1/10/Delft2014_LabjournalLandmine.pdf"> |
<p>Landmine Module</p> | <p>Landmine Module</p> | ||
</a></p> | </a></p> | ||
| - | <p><a href="https:// | + | <p><a href="https://static.igem.org/mediawiki/2014/b/b8/Delft2014_LabjournalEET.pdf"> |
<p>EET Module</p> | <p>EET Module</p> | ||
</a></p> | </a></p> | ||
| - | <p> <a href="https:// | + | <p> <a href="https://static.igem.org/mediawiki/2014/a/ae/Delft2014_LabjournalCurli.pdf"> |
<p>Curli Module</p> | <p>Curli Module</p> | ||
</a></p> | </a></p> | ||
<br> | <br> | ||
| - | <h3>Protocols</h3> | + | <h3>Protocols characterization</h3> |
| + | <p><a href="https://static.igem.org/mediawiki/2014/d/d9/Delft2014_Hemestainingprotocol.pdf"> | ||
| + | <p>Heme stain protocol</p> | ||
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/9/92/Delft2014_Membraneproteinpurification.pdf"> | ||
| + | <p>Membrane protein purification</p> | ||
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/a/a1/Delft2014_SDS-PAGEprotocol.pdf"> | ||
| + | <p>SDS-PAGE</p> | ||
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/f/f4/Delft2014_PlatereaderLD.pdf"> | ||
| + | <p>Plate reader Landmine Detection</p> | ||
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/1/16/Delft2014_curliplatereader.pdf"> | ||
| + | <p>Plate reader Conductive Curli</p> | ||
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/7/7a/Delft2014_ProtocolCongoRed.pdf"> | ||
| + | <p>Congo Red protocol</p> | ||
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/8/83/Delft2014_Gold_NP_and_CV_assay.pdf"> | ||
| + | <p>Gold nanoparticles and crystal violet protocol</p> | ||
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/d/dc/Delft2014_Bioreactor_protocol.pdf"> | ||
| + | <p>Bioreactor protocol</p> | ||
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/8/83/TUDelft_2014_Protocol_Promoter_Characterisation_iGEM_Wageningen.pdf"> | ||
| + | <p>Wageningen 2014 iGEM team collaboration protocol</p> | ||
| + | </a></p> | ||
| + | |||
| + | <h3> Protocols cloning </h3> | ||
<p> <a href="https://static.igem.org/mediawiki/2014/0/06/Delft2014_Miniprepprotocol.pdf"> | <p> <a href="https://static.igem.org/mediawiki/2014/0/06/Delft2014_Miniprepprotocol.pdf"> | ||
<p>Miniprep </p> | <p>Miniprep </p> | ||
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<p>Electroporation</p> | <p>Electroporation</p> | ||
</a></p> | </a></p> | ||
| - | <p><a href="https://static.igem.org/mediawiki/2014/ | + | <p><a href="https://static.igem.org/mediawiki/2014/e/e7/Delft2014_Gelelectrophoresis.pdf"> |
| - | <p> | + | <p>Gel electrophoresis</p> |
</a></p> | </a></p> | ||
| - | <p><a href="https://static.igem.org/mediawiki/2014/ | + | <p><a href="https://static.igem.org/mediawiki/2014/6/6c/Delft2014_Glycerolstockpreparation.pdf"> |
| - | <p> | + | <p>Glycerol stock preparation</p> |
</a></p> | </a></p> | ||
| - | <p><a href="https://static.igem.org/mediawiki/2014/ | + | <p><a href="https://static.igem.org/mediawiki/2014/e/ed/Delft2014_Makingcompetentcells.pdf"> |
| - | <p> | + | <p>Home-made competent cells</p> |
</a></p> | </a></p> | ||
| - | <p><a href="https://static.igem.org/mediawiki/2014/ | + | <p><a href="https://static.igem.org/mediawiki/2014/9/9b/Delft2014_MinElutegelextraction.pdf"> |
| - | <p> | + | <p>Extraction of DNA from gel</p> |
</a></p> | </a></p> | ||
| - | <p><a href="https://static.igem.org/mediawiki/2014/ | + | <p><a href="https://static.igem.org/mediawiki/2014/9/96/Delft2014_PCRpurificationQIAquickkit.pdf"> |
| - | <p> | + | <p>PCR purification</p> |
</a></p> | </a></p> | ||
| - | <p><a href="https://static.igem.org/mediawiki/2014/a/ | + | <p><a href="https://static.igem.org/mediawiki/2014/8/87/Delft2014_RestrictionofplasmidDNA.pdf"> |
| - | <p> | + | <p>Restriction of plasmid DNA</p> |
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/0/00/Delft2014_Restriction_of_PCR_product.pdf"> | ||
| + | <p>Restriction of PCR product</p> | ||
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/2/22/Delft2014_TransformationDH5alpha.pdf"> | ||
| + | <p>Transformation in commercial DH5alpha</p> | ||
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/4/45/Delft2014_Transformationhomemade.pdf"> | ||
| + | <p>Transformation in home-made competent cells</p> | ||
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/7/79/Delft2014_Trace_element_solution.pdf"> | ||
| + | <p>Trace element solution</p> | ||
| + | </a></p> | ||
| + | <p><a href="https://static.igem.org/mediawiki/2014/b/b1/Delft2014_Antibiotics.pdf"> | ||
| + | <p>Antibiotics stocks</p> | ||
</a></p> | </a></p> | ||
Latest revision as of 20:55, 17 October 2014
Notebook
In this notebook you can find all details on protocols, cloning and characterization experiments. Every link is a download link for a pdf file, be aware that the pdf files of the labjournals are quite large.
Labjournals
Protocols characterization
Plate reader Landmine Detection
Gold nanoparticles and crystal violet protocol
Wageningen 2014 iGEM team collaboration protocol
Protocols cloning
Transformation in commercial DH5alpha
