Team:TU Delft-Leiden/Project/Notebook

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    <h2> Notebook </h2>
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<p> In this notebook you can find all details on protocols, cloning and characterization experiments.
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Every link is a download link for a pdf file, be aware that the pdf files of the labjournals are quite large. </p>
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<div class="tableofcontents">
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<h2> Notebook </h2>
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<center> <h3> Contents </h3> </center>
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<p>
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In this notebook you can find all details on protocols, cloning and characterization experiments.
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Every link is a download link for a pdf file, be aware that the pdf files of the labjournals are quite large.
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</p>
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<ul>
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<br>
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    <li>
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          <p>Labjournals</p>
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          </a>
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          <ul>
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              <li>  
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<h3>Labjournals</h3>  
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                    <a href="https://static.igem.org/mediawiki/2014/2/29/Delft2014_Labjournal_General.pdf">
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<p><a href="https://static.igem.org/mediawiki/2014/2/29/Delft2014_Labjournal_General.pdf">
                     <p>General work </p>
                     <p>General work </p>
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                     </a>
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                     </a></p>
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              </li>
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<p><a href="https://static.igem.org/mediawiki/2014/1/10/Delft2014_LabjournalLandmine.pdf">
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              <li>
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                    <a href="https://2014.igem.org/Team:TU_Delft-Leiden/Project/Notebook/LabjournalLandmine">
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                     <p>Landmine Module</p>
                     <p>Landmine Module</p>
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                     </a>
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                     </a></p>
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              </li>
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<p><a href="https://static.igem.org/mediawiki/2014/b/b8/Delft2014_LabjournalEET.pdf">
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  <li>
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                    <a href="https://2014.igem.org/Team:TU_Delft-Leiden/Project/Notebook/LabjournalEET">
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                     <p>EET Module</p>
                     <p>EET Module</p>
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                     </a>
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                     </a></p>
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              </li>
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<p> <a href="https://static.igem.org/mediawiki/2014/a/ae/Delft2014_LabjournalCurli.pdf">
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  <li>
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                    <a href="https://2014.igem.org/Team:TU_Delft-Leiden/Project/Notebook/LabjournalCurli">
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                     <p>Curli Module</p>
                     <p>Curli Module</p>
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                     </a>
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                     </a></p>
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              </li>
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<br>
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          </ul>
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<h3>Protocols characterization</h3>
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    </li>
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<p><a href="https://static.igem.org/mediawiki/2014/d/d9/Delft2014_Hemestainingprotocol.pdf">
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<li>
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                    <p>Heme stain protocol</p>
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          <p>Protocols</p>
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                    </a></p>
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          </a>
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<p><a href="https://static.igem.org/mediawiki/2014/9/92/Delft2014_Membraneproteinpurification.pdf">
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                    <p>Membrane protein purification</p>
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          <ul>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/a/a1/Delft2014_SDS-PAGEprotocol.pdf">
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                    <p>SDS-PAGE</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/f/f4/Delft2014_PlatereaderLD.pdf">
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                    <p>Plate reader Landmine Detection</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/1/16/Delft2014_curliplatereader.pdf">
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                    <p>Plate reader Conductive Curli</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/7/7a/Delft2014_ProtocolCongoRed.pdf">
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                    <p>Congo Red protocol</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/8/83/Delft2014_Gold_NP_and_CV_assay.pdf">
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                    <p>Gold nanoparticles and crystal violet protocol</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/d/dc/Delft2014_Bioreactor_protocol.pdf">
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                    <p>Bioreactor protocol</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/8/83/TUDelft_2014_Protocol_Promoter_Characterisation_iGEM_Wageningen.pdf">
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                    <p>Wageningen 2014 iGEM team collaboration protocol</p>
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                    </a></p>
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              <li>  
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<h3> Protocols cloning </h3>
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                    <a href="https://2014.igem.org/Team:TU_Delft-Leiden/Project/Notebook/ProtocolMiniprep">
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<p>    <a href="https://static.igem.org/mediawiki/2014/0/06/Delft2014_Miniprepprotocol.pdf">
                     <p>Miniprep </p>
                     <p>Miniprep </p>
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                     </a>
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                     </a></p>
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              </li>
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<p><a href="https://static.igem.org/mediawiki/2014/a/a5/Delft2014_Electroporationprotocol.pdf">
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              <li>
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                    <a href="https://2014.igem.org/Team:TU_Delft-Leiden/Project/Notebook/ProtocolElectroporation">
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                     <p>Electroporation</p>
                     <p>Electroporation</p>
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                     </a>
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                     </a></p>
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              </li>
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<p><a href="https://static.igem.org/mediawiki/2014/e/e7/Delft2014_Gelelectrophoresis.pdf">
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                    <p>Gel electrophoresis</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/6/6c/Delft2014_Glycerolstockpreparation.pdf">
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                    <p>Glycerol stock preparation</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/e/ed/Delft2014_Makingcompetentcells.pdf">
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                    <p>Home-made competent cells</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/9/9b/Delft2014_MinElutegelextraction.pdf">
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                    <p>Extraction of DNA from gel</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/9/96/Delft2014_PCRpurificationQIAquickkit.pdf">
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                    <p>PCR purification</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/8/87/Delft2014_RestrictionofplasmidDNA.pdf">
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                    <p>Restriction of plasmid DNA</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/0/00/Delft2014_Restriction_of_PCR_product.pdf">
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                    <p>Restriction of PCR product</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/2/22/Delft2014_TransformationDH5alpha.pdf">
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                    <p>Transformation in commercial DH5alpha</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/4/45/Delft2014_Transformationhomemade.pdf">
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                    <p>Transformation in home-made competent cells</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/7/79/Delft2014_Trace_element_solution.pdf">
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                    <p>Trace element solution</p>
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                    </a></p>
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<p><a href="https://static.igem.org/mediawiki/2014/b/b1/Delft2014_Antibiotics.pdf">
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                    <p>Antibiotics stocks</p>
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                    </a></p>
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Latest revision as of 20:55, 17 October 2014


Notebook

In this notebook you can find all details on protocols, cloning and characterization experiments. Every link is a download link for a pdf file, be aware that the pdf files of the labjournals are quite large.


Labjournals

General work

Landmine Module

EET Module

Curli Module


Protocols characterization

Heme stain protocol

Membrane protein purification

SDS-PAGE

Plate reader Landmine Detection

Plate reader Conductive Curli

Congo Red protocol

Gold nanoparticles and crystal violet protocol

Bioreactor protocol

Wageningen 2014 iGEM team collaboration protocol

Protocols cloning

Miniprep

Electroporation

Gel electrophoresis

Glycerol stock preparation

Home-made competent cells

Extraction of DNA from gel

PCR purification

Restriction of plasmid DNA

Restriction of PCR product

Transformation in commercial DH5alpha

Transformation in home-made competent cells

Trace element solution

Antibiotics stocks

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