Team:Bordeaux/NotebookJune6

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We try a non-selective culture with our bacteria.
We try a non-selective culture with our bacteria.
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Latest revision as of 20:52, 17 October 2014

6th June

Molecular biology

  • We need BL21 E.coli without plasmid for have new competent BL21
    We try a non-selective culture with our bacteria.
    • schema1

  • Plasmid extraction by QIAprep Spin Miniprep Kit from QIAGEN of our ELPs.
    • schema1

  • Digestion by BamH1 and EcoR1, ligation and transformation by electroporation and by heat shock of our bacteria with low copy IGEM backbone pSB1C3 (2014 Kit Plate 4, well 4B)
    • Transformation by heat shock
      1. Plate of LB and pSB1C3
      2. Plate of LB + Chlo and pSB1C3
      3. Plate of LB + Chlo without DNA
      4. Plate of LB + Amp and pSBK-

    • Transformation by electroporation: recuperation on
      1. Plate of LB and pSB1C3
      2. Plate of LB + Chlo and pSB1C3
      3. Plate of LB + Chlo without DNA
      4. Plate of LB + Amp and pSBK-

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