Team:TU Delft-Leiden/Project/Life science/EET

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<h2> Extracellular Electron Transport module</h2>
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    <h2>Module Electron Transport</h2>
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    <p>
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        Information with respect to literature consulted regarding the Modules is referred to under Context. Also, each of the three complementary Modules is equipped with an Integration of Departments, in which it is described how the Departments Modeling, Experimental Work and Microfluidics interact. Furthermore, each Module contains information on Cloning and results are presented under Characterization.
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      <ul> Module Electron Transport
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        <ul>
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            <li><a href="/Team:TU_Delft-Leiden/Project/Life_science/EET/theory">Context</a></li>
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            <li><a href="/Team:TU_Delft-Leiden/Project/Life_science/EET/integration">Integration of Departments</a></li>
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            <li><a href="/Team:TU_Delft-Leiden/Project/Life_science/EET/cloning">Cloning</a></li>
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            <li><a href="/Team:TU_Delft-Leiden/Project/Life_science/EET/characterisation">Characterization</a></li>
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        </ul>
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Keen to see our <b>conclusions</b> for this module?  See the list below!
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<li>The <i> mtrCAB</i> genes under control of the weakened T7lacO promoter were successfully BioBricked.</li>
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<li>The <i> ccm </i> genes under control of the pFAB640 promoter were successfully BioBricked. </li>
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<li>Expression of both our <i>mtrCAB</i> BioBrick and <i>ccm</i> BioBrick results in Extracellular Electron Transport. </li>
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<h3> Background Information</h3>
 
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Want to know how we came to these conclusions? Go to our <a href=https://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/EET/characterisation><b>Characterization</b></a> page!</p>
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    <p>
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        Interested in one of our other Modules? Navigate to the&nbsp; <a href="https://2014.igem.org/Team:TU_Delft-Leiden/WetLab/landmine"> <b> Module Landmine Detection </b> </a> page and discover how we tweaked <i> E. coli</i> to let it respond on landmines. Interested in living materials? Go to our  <a href="https://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/curli"> <b> Module Conductive Curli </b> </a> and find out how you can combine the benefits of both living- and non-living materials.
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<figure>
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<img src=
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"https://static.igem.org/mediawiki/2014/8/86/Module_electron_transport_transfer_pathway_with_Mtr_protein_complex.jpg" width="100%" height="100%">
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<figcaption>
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<br>
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<h2> Cloning Strategy and Characterisation of this module</h2>
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Figure 1: General scheme of biosensory plug-and-play E.coli cell responsive to, in this particular case, TNT. Generated is a measureable electrical current via the implemented extracellular electron transport pathway.
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  <li> <a href="/Team:TU_Delft-Leiden/Project/Life_science/EET/cloning"><p> Cloning </p>
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        </a>
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</li>
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<li> <a href="/Team:TU_Delft-Leiden/Project/Life_science/EET/characterisation"><p> Characterisation </p>
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<h3> References </h3>
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Latest revision as of 20:49, 17 October 2014

Module Electron Transport

Information with respect to literature consulted regarding the Modules is referred to under Context. Also, each of the three complementary Modules is equipped with an Integration of Departments, in which it is described how the Departments Modeling, Experimental Work and Microfluidics interact. Furthermore, each Module contains information on Cloning and results are presented under Characterization.

Keen to see our conclusions for this module? See the list below!

  • The mtrCAB genes under control of the weakened T7lacO promoter were successfully BioBricked.
  • The ccm genes under control of the pFAB640 promoter were successfully BioBricked.
  • Expression of both our mtrCAB BioBrick and ccm BioBrick results in Extracellular Electron Transport.

Want to know how we came to these conclusions? Go to our Characterization page!


Interested in one of our other Modules? Navigate to the  Module Landmine Detection page and discover how we tweaked E. coli to let it respond on landmines. Interested in living materials? Go to our Module Conductive Curli and find out how you can combine the benefits of both living- and non-living materials.



Figure 1: General scheme of biosensory plug-and-play E.coli cell responsive to, in this particular case, TNT. Generated is a measureable electrical current via the implemented extracellular electron transport pathway.
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