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Team:Evry/Notebook/Transposons/15.10.14
From 2014.igem.org
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== Verification testing of pnK2 in <i>Pseudovibrio denitrificans</i> | == Verification testing of pnK2 in <i>Pseudovibrio denitrificans</i> | ||
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| + | 16 clones of <i>Pseudovibrio denitrificans</i> were grown in 3 ml MB, 30 C°, 24h | ||
| + | |||
| + | DNA extraction: | ||
| + | |||
| + | Invitrogen DNA extraction kit used with the Gram - protocol. | ||
| + | nanodrops gave value between 80 ng/ul & 130 ng/ul | ||
| + | |||
| + | PCR: | ||
| + | |||
Revision as of 20:45, 17 October 2014
== 14.10.2014 ligation==
it gave only one clone.
Clone culture:
5 ml LB + 5 ul Kan (25 mg/ml) - 37 C°
== Transformation Pseudovibrio denitrificans & Dh5 alpha with mRFP in BBa_1413044==
electrocompetent cells:
1 mm cuvette; 1800 V
recuperation media: MB for Pseudovibrio denitrificans , LB for DH5alpha
plating:
MB agar for Pseudovibrio denitrificans
LB agar for LB for DH5alpha
== Transformation Pseudovibrio denitrificans & Dh5 alpha with BBa_1413001 in BBa_1413044==
electrocompetent cells:
1 mm cuvette; 1800 V
recuperation media: MB for Pseudovibrio denitrificans , LB for DH5alpha
plating:
MB agar for Pseudovibrio denitrificans
LB agar for LB for DH5alpha
== Verification testing of pnK2 in Pseudovibrio denitrificans
16 clones of Pseudovibrio denitrificans were grown in 3 ml MB, 30 C°, 24h
DNA extraction:
Invitrogen DNA extraction kit used with the Gram - protocol.
nanodrops gave value between 80 ng/ul & 130 ng/ul
PCR:
