Team:Michigan/Collaborations/

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TU Braunschweig & Dr. Hust

Once our project was designed, we seeked overview from specialists in the field. We reached out to Dr. Hust von Technische Universität Braunschweig (Department of Biotechnology). Dr. Hust specializes in building antibody gene libraries and purifying antibody scFvs in E.coli. Through a skype conference he looked over our project, advised experimental design, and helped us build a story around antibody scFv secretion in E.coli. After this discussion, we focused our project on Salmonella sensing and Dr. Hust sent us antibody scFvs targeting Salmonella proteins. These scFv were produced by Dr. Hust through antibody phage display. We wish to thank our collaborator for receiving us with attention and for such strong contribution to our work

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+	<p style="position:absolute;top:88px;width:571px"> Once our project was designed, we seeked overview from specialists in the field. We reached out to Dr. Hust von Technische Universität Braunschweig (Department of Biotechnology). Dr. Hust specializes in building antibody gene libraries and purifying antibody scFvs in E.coli. Through a skype conference he looked over our project, advised experimental design, and helped us build a story around antibody scFv secretion in E.coli. After this discussion, we focused our project on Salmonella sensing and Dr. Hust sent us antibody scFvs targeting Salmonella proteins. These scFv were produced by Dr. Hust through antibody phage display. We wish to thank our collaborator for receiving us with attention and for such strong contribution to our work</p>
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+	<p style="position:absolute;top:355px;width:884px"> Through this meeting with Covance, we were able to <b>gauge the practicality </b> of and <b>modify our project</b> to demonstrate the possible applications of our construct. She advised against an aspect of our project that aimed to improve current probiotic supplements with secretable antibodies. For instance, probiotic supplements could have implications in the cattle industry. <i>E. coli</i> is a naturally occurring bacterium in the intestinal microbiome of cattle, and probiotics are used extensively in the industry to promote good health in the cattle. By inoculating currently used probiotics with <i>E. coli</i> capable of secreting antibodies specific to antigens on the surface of Salmonella cells, we could effect agglutination and removal of harmful bacteria. However, Christine advised against this due to the required resources, length of project, and complications with animal testing. We were able to <b> narrow the focus </b> of our project by concentrating on producing overexpressed antibodies that could be secreted and purified easily. By allowing simplified antibody purification, we could make improvements in the time it takes to go from demand to detection. This method seeks to go beyond the bench by allowing others in both <b>academia and industry</b> to obtain complexly folded proteins easily. This collaboration with Covance also <b>guided our Economic Analysis</b>, as we sought to determine the major limitations of antibody production in the biopharmaceutical market. </p>
+	<p style="position:absolute;top:595px;left:337px"><font size="6"> Public Health Implications </font></p>
+	<p style="position:absolute;top:635px;width:884px"> Salmonella, the cause of gastroenteritis, poses potential risks of mild to life threatening infections.  In the United States, the Centers for Disease Control and Prevention (CDC) estimates that food-borne Salmonella infections cause 1.2 million illnesses yearly, with more than 23,000 hospitalizations and 450 deaths.  The US Department of Agriculture (USDA) estimated that Salmonella cost the nation about $2.65 billion a year [3]. Consequently, detecting and treating pathogenic Salmonella is important to food and health industries [1]. </p>
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+	<p style="position:absolute;top:1000px;width:884px"> Environmental samples, food products, and organisms are all tested for Salmonella.  Current detection methods require culturing samples on selective media for preliminary identification that must later be confirmed using biochemical and serological testing.  Biochemical indicators include fermentation of glucose, negative urease reaction, lysine decarboxylase, negative indole test, hydrogen sulfide production, and fermentation of dulcitol.  Serotyping examines for flagellar (H) and somatic (O) antigens.  Salmonella can also be detected using an ELISA, where the Salmonella antigen is immobilized, bound with specific antibodies, and washed to remove any proteins or other bound antibodies.  The result is a visible signal that quantifies the amount of Salmonella antigen present [2].  The cost of the antibodies required in an ELISA can be reduced by using our OsmY purification system.
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+ 	<p style="position:absolute;top:1150px;width:884px"> References: </br>
+[1] "Salmonella." Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 28 Aug. 2014. Web. 17 Oct. 2014. <http://www.cdc.gov/salmonella/>.
+</br>
+[2] Santiago-Felipe, S., Tortajada-Genaro, L. A., Puchades, R. & Maquieira, A. Recombinase polymerase and enzyme-linked immunosorbent assay as a DNA amplification-detection strategy for food analysis. Analytica chimica acta. 811, 81–7 (2014).
+</br>
+[3] Ross, Robert. "USDA Estimates E Coli, Salmonella Costs at $3.1 Billion." CIDRAP. University of Minnesota, 24 May 2010. Web. 25 Aug. 2014. <http://www.cidrap.umn.edu/news-perspective/2010/05/usda-estimates-e-coli-salmonella-costs-31-billion>.
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