Team:Brasil-SP/Notebook
From 2014.igem.org
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- | < | + | <!--main content --> |
- | < | + | <table width="70%" align="center"> |
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- | < | + | <h1 align=center>Main Assembly Map</h1> |
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- | </ | + | <p><div align="justify"> The main assembly map describes all the constructions that would be done in the project, but some of them have not been performed in time of the iGEM deadline. The succesfully constructed assemblies is indicated by a green check point and the unsuccessfull constructions are indicated by a red "X". Click on these assemblies to access the lab form.</div></p> |
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+ | .map_image .map_link:hover {border: dashed 2px #d7ccea;} | ||
+ | .map_image #map_link_1 { width: 120px; height: 82px; top: 15px; left: 1px;} | ||
+ | .map_image #map_link_2 { width: 86px; height: 83px; top: 17px; left: 136px; } | ||
+ | .map_image #map_link_3 { width: 223px; height: 84px; top: 16px; left: 229px; } | ||
+ | .map_image #map_link_4 { width: 138px; height: 86px; top: 14px; left: 464px; } | ||
+ | .map_image #map_link_5 { width: 113px; height: 85px; top: 14px; left: 616px; } | ||
+ | .map_image #map_link_6 { width: 256px; height: 89px; top: 125px; left: 140px; } | ||
+ | .map_image #map_link_7 { width: 351px; height: 87px; top: 258px; left: 221px; } | ||
+ | .map_image #map_link_8 { width: 425px; height: 91px; top: 404px; left: 47px; } | ||
+ | .map_image #map_link_9 { width: 111px; height: 80px; top: 153px; left: 978px; } | ||
+ | .map_image #map_link_10 { width: 168px; height: 81px; top: 153px; left: 805px; } | ||
+ | .map_image #map_link_11 { width: 127px; height: 83px; top: 129px; left: 629px; } | ||
+ | </style> | ||
+ | <div class="map_image" style="background-image: url('https://static.igem.org/mediawiki/2014/8/87/Mapa.png');"> | ||
+ | <a class="map_link" id="map_link_1" title="" href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a> | ||
+ | <a class="map_link" id="map_link_2" title="" href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a> | ||
+ | <a class="map_link" id="map_link_3" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> | ||
+ | <a class="map_link" id="map_link_4" title="" href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a> | ||
+ | <a class="map_link" id="map_link_5" title="" href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a> | ||
+ | <a class="map_link" id="map_link_6" title="" href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> | ||
+ | <a class="map_link" id="map_link_7" title="" href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a> | ||
+ | <a class="map_link" id="map_link_8" title="" href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a> | ||
+ | <a class="map_link" id="map_link_9" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a> | ||
+ | <a class="map_link" id="map_link_10" title="" href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> | ||
+ | <a class="map_link" id="map_link_11" title="" href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> | ||
+ | </div> | ||
- | + | <h1 align=center>Assemblies forms</h1> | |
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<p><strong>Assembly form template</strong></p> | <p><strong>Assembly form template</strong></p> | ||
<p>This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)</p> | <p>This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)</p> | ||
- | + | <div align=center> | |
<a href="https://static.igem.org/mediawiki/2014/e/eb/Assembly_Form.pdf"><img src="https://static.igem.org/mediawiki/2014/3/32/Assembly_form_pg3.png" style="width:700px;height:400px"/></a> | <a href="https://static.igem.org/mediawiki/2014/e/eb/Assembly_Form.pdf"><img src="https://static.igem.org/mediawiki/2014/3/32/Assembly_form_pg3.png" style="width:700px;height:400px"/></a> | ||
+ | </div> | ||
- | + | <h1 align=center><strong>Life Inside the LAB</strong></h1> | |
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- | <thead><h2>July</h2> | + | <thead><h2 align=center>July</h2> |
</thead> | </thead> | ||
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<table> | <table> | ||
<tr> | <tr> | ||
- | <th>Monday</th> | + | <th style="background-color:#bfacc0">Monday</th> |
- | <th>Tuesday</th> | + | <th style="background-color:#bfacc0">Tuesday</th> |
- | <th>Wednesday</th> | + | <th style="background-color:#bfacc0">Wednesday</th> |
- | <th>Thursday</th> | + | <th style="background-color:#bfacc0">Thursday</th> |
- | <th>Friday</th> | + | <th style="background-color:#bfacc0">Friday</th> |
- | <th>Saturday</th> | + | <th style="background-color:#bfacc0">Saturday</th> |
- | <th>Sunday</th> | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
<td>30/06 | <td>30/06 | ||
<ul> | <ul> | ||
- | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> lasR (BBa_C0079). The purpose of this PCR was to add the RBS (BBa_K143021), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called BBa_K1521001.</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> lasR (<a href="http://parts.igem.org/Part:BBa_C0079" style="color:#3ab473">BBa_C0079</a>). The purpose of this PCR was to add the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021</a>), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called <a href="http://parts.igem.org/Part:BBa_K1521001" style="color:#3ab473">BBa_K1521001</a>.</li> |
</ul> | </ul> | ||
</td> | </td> | ||
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<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks:</li> | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks:</li> | ||
<ul> | <ul> | ||
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li> |
- | <li>BBa_K081001</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li> |
- | <li>BBa_E0840</li> | + | <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li> |
</ul> | </ul> | ||
<li>Inoculum of the Biobricks sent by iGEM HQ:</li> | <li>Inoculum of the Biobricks sent by iGEM HQ:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K316037</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li> |
- | <li>BBa_K316018</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li> |
- | <li>BBa_K316015</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks sent by the Imperial College Team:</li> | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks sent by the Imperial College Team:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016<a/></li> |
- | <li>BBa_K143031</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143031" style="color:#3ab473">BBa_K143031<a/></li> |
</ul> | </ul> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification of the plasmidial DNA samples and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li> |
<ul> | <ul> | ||
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li> |
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li> |
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li> |
- | <li>BBa_K081001</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li> |
- | <li>BBa_E0840</li> | + | <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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<td>07/07 | <td>07/07 | ||
<ul> | <ul> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li> |
<ul> | <ul> | ||
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li> |
- | <li>BBa_K081001</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li> |
- | <li>BBa_E0840</li> | + | <li<a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li> |
</ul> | </ul> | ||
<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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<td>10/07 | <td>10/07 | ||
<ul> | <ul> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_B0015 (restriction analysis fail, repeat)</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a> (restriction analysis fail, repeat)</li> |
</ul> | </ul> | ||
- | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> the qteE gene for amplification. We also added the RBS (BBa_K143021) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called BBa_K1521000.</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> the qteE gene for amplification. We also added the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021,</a>) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called <a href="http://parts.igem.org/Part:BBa_K1521000" style="color:#3ab473">BBa_K1521000</a>.</li> |
<li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of RBS+lasR in PTZ57R/T vector (instaclone kit)</li> | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of RBS+lasR in PTZ57R/T vector (instaclone kit)</li> | ||
</ul> | </ul> | ||
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<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> | ||
<ul> | <ul> | ||
- | <li>RBS + lasR (BBa_C0079)</li> | + | <li>RBS + lasR (<a href="http://parts.igem.org/Part:BBa_C0079"style="color:#3ab473">BBa_C0079</a>)</li> |
</ul> | </ul> | ||
<li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a>:</li> | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a>:</li> | ||
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<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> <a href="" style="color:#3ab473"> BBa_J04450</a></a></li> |
</ul> | </ul> | ||
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 468: | Line 259: | ||
<td>16/07 | <td>16/07 | ||
<ul> | <ul> | ||
- | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_K823003</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li> |
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
</ul> | </ul> | ||
<li>Electrophoresis analysis:</li> | <li>Electrophoresis analysis:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 481: | Line 272: | ||
<td>17/07 | <td>17/07 | ||
<ul> | <ul> | ||
- | <li>Glycerol Stock and <a href=" | + | <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:<li> |
<ul> | <ul> | ||
- | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li> |
<li>RBS+lasR</li> | <li>RBS+lasR</li> | ||
- | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_J04450 (EP)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li> |
- | <li>BBa_J04450 (XS)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li> |
</ul> | </ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K823003</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li> |
</ul> | </ul> | ||
<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
<ul> | <ul> | ||
- | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 504: | Line 295: | ||
<td>18/07 | <td>18/07 | ||
<ul> | <ul> | ||
- | <li>Glycerol Stock and <a href=" | + | <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li> |
- | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
<li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a>:</li> | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a>:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_J04450 (EP)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li> |
- | <li>BBa_J04450 (XS)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li> |
</ul> | </ul> | ||
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K823003</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 527: | Line 318: | ||
<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> | ||
<ul> | <ul> | ||
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
</ul> | </ul> | ||
Line 544: | Line 335: | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li> |
<ul> | <ul> | ||
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
Line 567: | Line 358: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
<li>Prepare RBS+lasR and RBS+qteE for sequencing</li> | <li>Prepare RBS+lasR and RBS+qteE for sequencing</li> | ||
Line 578: | Line 369: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
</ul> | </ul> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 589: | Line 380: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
</ul> | </ul> | ||
Line 602: | Line 393: | ||
<ul> | <ul> | ||
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (+NdeI enzyme)</li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (+NdeI enzyme)</li> | ||
- | <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that | + | <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.</li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
Line 641: | Line 432: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
</ul> | </ul> | ||
- | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> BBa_K143055 for RBS removal</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for RBS removal</li> |
<li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :) </li> | <li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :) </li> | ||
- | <li>PCR of Plac BBa_K143055 was followed by a <a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> in the PUC19 vector</li> | + | <li>PCR of Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> was followed by a <a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> in the PUC19 vector</li> |
</ul> | </ul> | ||
</td> | </td> | ||
Line 651: | Line 442: | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | |||
</tbody> | </tbody> | ||
- | <thead><h2>August</h2> | + | <thead><h2 align=center>August</h2> |
</thead> | </thead> | ||
Line 662: | Line 458: | ||
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
- | <th>Monday</th> | + | <th style="background-color:#bfacc0">Monday</th> |
- | <th>Tuesday</th> | + | <th style="background-color:#bfacc0">Tuesday</th> |
- | <th>Wednesday</th> | + | <th style="background-color:#bfacc0">Wednesday</th> |
- | <th>Thursday</th> | + | <th style="background-color:#bfacc0">Thursday</th> |
- | <th>Friday</th> | + | <th style="background-color:#bfacc0">Friday</th> |
- | <th>Saturday</th> | + | <th style="background-color:#bfacc0">Saturday</th> |
- | <th>Sunday</th> | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
<td></td> | <td></td> | ||
Line 678: | Line 474: | ||
<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
Line 685: | Line 481: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
</ul> | </ul> | ||
Line 700: | Line 496: | ||
<li>E. coli DH5-alpha</li> | <li>E. coli DH5-alpha</li> | ||
<li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li> | ||
- | <li>Plac BBa_K143055</li> | + | <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
<ul> | <ul> | ||
- | <li>BBa_P0312</li> | + | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | ||
Line 716: | Line 512: | ||
<ul> | <ul> | ||
<li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li> | <li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li> |
<ul> | <ul> | ||
- | <li>Plac BBa_K143055</li> | + | <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
<ul> | <ul> | ||
- | <li>BBa_P0312</li> | + | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | ||
<ul> | <ul> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
- | <li>And once again! <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (this BBa_K316016 is tough)</li> | + | <li>And once again! <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (this <a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a> is tough)</li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 734: | Line 530: | ||
<td>07/08 | <td>07/08 | ||
<ul> | <ul> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_P0312</li> | + | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 746: | Line 542: | ||
<td>11/08 | <td>11/08 | ||
<ul> | <ul> | ||
- | <li>Prepare Plac BBa_K143055 for sequencing</li> | + | <li>Prepare Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for sequencing</li> |
<li>Analyse the RBS+qteE and RBS+lasR sequencing file</li> | <li>Analyse the RBS+qteE and RBS+lasR sequencing file</li> | ||
</ul> | </ul> | ||
Line 838: | Line 634: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a></li> |
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
</ul> | </ul> | ||
Line 848: | Line 644: | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">restriction analysis</a> adding the enzyme NdeI | + | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">restriction analysis</a> adding the enzyme NdeI.</li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 859: | Line 655: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
<ul> | <ul> | ||
- | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li> |
- | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
- | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> BBa_K143055 with higher melting temperature | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> with higher melting temperature.</li> |
<ul> | <ul> | ||
</td> | </td> | ||
Line 895: | Line 691: | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
- | <thead><h2>September</h2> | + | <thead><h2 align=center>September</h2> |
</thead> | </thead> | ||
Line 904: | Line 703: | ||
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
- | < | + | <th style="background-color:#bfacc0">Monday</th> |
- | < | + | <th style="background-color:#bfacc0">Tuesday</th> |
- | < | + | <th style="background-color:#bfacc0">Wednesday</th> |
- | < | + | <th style="background-color:#bfacc0">Thursday</th> |
- | < | + | <th style="background-color:#bfacc0">Friday</th> |
- | < | + | <th style="background-color:#bfacc0">Saturday</th> |
- | < | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
<td>01/09 | <td>01/09 | ||
Line 916: | Line 715: | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a> | ||
<ul> | <ul> | ||
- | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a> of the part BII with EcoRV | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a> of the part BII with EcoRV. |
</ul> | </ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | ||
Line 961: | Line 760: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
</ul> | </ul> | ||
Line 973: | Line 772: | ||
<td>08/09 | <td>08/09 | ||
<ul> | <ul> | ||
- | <li>QuickChange PCR to mutate the cleavage site of the | + | <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li> |
<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li> | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li> | ||
</ul> | </ul> | ||
Line 981: | Line 780: | ||
<ul> | <ul> | ||
<li>The QuickChange protocol didn't work well. So try again with more attention!</li> | <li>The QuickChange protocol didn't work well. So try again with more attention!</li> | ||
- | <li>QuickChange PCR to mutate the cleavage site of the | + | <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li> |
<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li> | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
Line 994: | Line 793: | ||
<td>11/09 | <td>11/09 | ||
<ul> | <ul> | ||
- | <li>Prepare inoculum of the mutated BBa_K316037</li> | + | <li>Prepare inoculum of the mutated <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li> |
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
<a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>, | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>, | ||
Line 1,012: | Line 811: | ||
<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
<ul> | <ul> | ||
- | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a></li> |
</ul> | </ul> | ||
- | <li>We had far to many difficulties trying to remove the RBS from the BBa_K143055, so we decided to synthesize it using PCR.</li> | + | <li>We had far to many difficulties trying to remove the RBS from the <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a>, so we decided to synthesize it using PCR.</li> |
- | <li>PCR for | + | <li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf" style="color:#f05151">PCR for synthesis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> (<a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> without RBS)</li> |
</ul> | </ul> | ||
</td> | </td> | ||
Line 1,038: | Line 837: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
</ul> | </ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
Line 1,061: | Line 860: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
</ul> | </ul> | ||
- | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> of the BBa_K143015</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li> |
</ul> | </ul> | ||
</td> | </td> | ||
Line 1,072: | Line 871: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
</ul> | </ul> | ||
Line 1,079: | Line 878: | ||
<td>17/09 | <td>17/09 | ||
<ul> | <ul> | ||
- | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of the synthesized BBa_K143015 in TOPO vector followed by <a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | + | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of the synthesized <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> in TOPO vector followed by <a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
Line 1,089: | Line 888: | ||
<td>18/09 | <td>18/09 | ||
<ul> | <ul> | ||
- | <li>Replate BBa_K143015. We forgot the X-gal :(</li> | + | <li>Replate <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a>. We forgot the X-gal :(</li> |
</ul> | </ul> | ||
<ul> | <ul> | ||
Line 1,101: | Line 900: | ||
<td>22/09 | <td>22/09 | ||
<ul> | <ul> | ||
- | <li>Inoculum of the BBa_K143015</li> | + | <li>Inoculum of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li> |
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> | ||
<ul> | <ul> | ||
Line 1,115: | Line 914: | ||
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
</ul> | </ul> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li> |
</ul> | </ul> | ||
</td> | </td> | ||
Line 1,141: | Line 940: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
</ul> | </ul> | ||
Line 1,184: | Line 983: | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | |||
- | <thead><h2>October</h2> | + | <thead><h2 align=center>October</h2> |
</thead> | </thead> | ||
Line 1,193: | Line 997: | ||
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
- | < | + | <th style="background-color:#bfacc0">Monday</th> |
- | < | + | <th style="background-color:#bfacc0">Tuesday</th> |
- | < | + | <th style="background-color:#bfacc0">Wednesday</th> |
- | < | + | <th style="background-color:#bfacc0">Thursday</th> |
- | < | + | <th style="background-color:#bfacc0">Friday</th> |
- | < | + | <th style="background-color:#bfacc0">Saturday</th> |
- | < | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
<td></td> | <td></td> | ||
Line 1,210: | Line 1,014: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 1,228: | Line 1,032: | ||
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
</ul> | </ul> | ||
- | <li>Assemblies KIX, | + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, |
+ | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
+ | <ahref="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
<ul> | <ul> | ||
<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
Line 1,234: | Line 1,040: | ||
</ul> | </ul> | ||
</td> | </td> | ||
- | <td>07/10</td> | + | <td>07/10 |
- | <td>08/10</td> | + | <ul> |
- | <td>09/10</td> | + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, |
- | <td>10/10</td> | + | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and |
+ | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
+ | <ul> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
+ | </ul> | ||
+ | </ul> | ||
+ | </td> | ||
+ | <td>08/10 | ||
+ | <ul> | ||
+ | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
+ | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
+ | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
+ | <ul> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
+ | </ul> | ||
+ | <ul> | ||
+ | </td> | ||
+ | <td>09/10 | ||
+ | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
+ | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
+ | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
+ | <ul> | ||
+ | <li>Inoculum</li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | <td>10/10 | ||
+ | <ul> | ||
+ | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
+ | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
+ | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
+ | <ul> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
+ | </ul> | ||
+ | </ul> | ||
+ | </td> | ||
<td>11/10</td> | <td>11/10</td> | ||
<td>12/10</td> | <td>12/10</td> | ||
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</html> | </html> | ||
+ | {{:Team:Brasil-SP/Templates/Footer}} |
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