Team:Brasil-SP/Notebook

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<h1 >WELCOME TO iGEM 2014! </h1>
 
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<p>Your team has been approved and you are ready to start the iGEM season!
 
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
 
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<br>
 
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Brasil-SP/Notebook&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 
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<a href="https://2014.igem.org/Team:Brasil-SP"style="color:#000000">Home </a> </td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Team"style="color:#000000"> Team </a> </td>
 
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Brasil-SP"style="color:#000000"> Official Team Profile </a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Project"style="color:#000000"> Project</a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:Brasil-SP/Modeling"style="color:#000000"> Modeling</a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Notebook"style="color:#000000"> Notebook</a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Safety"style=" color:#000000"> Safety </a></td>
 
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<body>
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<a href="https://2014.igem.org/Team:Brasil-SP/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<h1 align=center>Main Assembly Map</h1>
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<p><div align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The main assembly map describes all the constructions that would be done in the project, but some of them have not been performed in time of the iGEM deadline. The succesfully constructed assemblies is indicated by a green check point and the unsuccessfull constructions are indicated by a red "X". Click on these assemblies to access the lab form.</div></p>
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<a class="map_link" id="map_link_1" title="" href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>
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<a class="map_link" id="map_link_2" title="" href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>
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<a class="map_link" id="map_link_3" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>
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<a class="map_link" id="map_link_4" title="" href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>
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<a class="map_link" id="map_link_5" title="" href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>
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<a class="map_link" id="map_link_6" title="" href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>
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<a class="map_link" id="map_link_7" title="" href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>
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<a class="map_link" id="map_link_8" title="" href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>
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<a class="map_link" id="map_link_9" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>
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<a class="map_link" id="map_link_10" title="" href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>
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        <a class="map_link" id="map_link_11" title="" href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a>
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<h1 align=center>Assemblies forms</h1>
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<p><strong>Assembly form template</strong></p>
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<p>This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)</p>
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<tr><td colspan="3"> <h3>Notebook</h3></td></tr>
 
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<h1 align=center><strong>Life Inside the LAB</strong></h1>
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<td width="45%"  valign="top">
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<p>You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. </p>
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  <h1> <strong>Lab Protocols</strong> </h1>
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  <h2> Competent Cell with Calcium Chloride </h2>
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      <ol>
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        <li><h3>Materials</h3></li>
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            <ul>
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              <li> Solid LB medium - 1 plate </li>
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              <li> Liquid LB medium </li>
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              <li> 2 250ml centrifuge tubes </li>
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              <li> Centrifuge </li>
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              <li> Cell sample (DH5-alpha in our case) </li>
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              <li> Autoclave </li>
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              <li> Inoculation loop </li>
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              <li> Liquid nitrogen </li>
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              <li> 37°C oven </li>
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              <li> CaCl<sub>2</sub>:</li>
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                  <ul>
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                    <li> 60mM CaCl<sub>2</sub> </li>
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                    <li> 10mM HEPES </li>
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                    <li> 15% glicerol </li>
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                    <li> H<sub>2</sub>O to 100ml </li>
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            </ul>
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        <li><h3> Methods </h3> </li>
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        <p><strong>Day 1</strong></p>
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            <ol>
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              <li> Risk the LB plate with the cell sample (DH5-alpha) </li>
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              <li> Incubate the plate at 37°C overnight </li>
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            </ol>
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        <p><strong>Day 2</strong></p>
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            <ol>
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              <li> Sterilize in the Autoclave </li>
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              <ul>
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                  <li> 100ml of liquid LB medium </li>
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                  <li> 100 ml of CaCl<sub>2</sub>
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                  <li> 2 250ml centrifuge tubes </li>
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              </ul>
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              <li> Prepare a 6ml LB inoculum with a DH5-alpha colony and incubate at 37°C/250rpm overnight</li>
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            </ol>
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        <p><strong>Day 3</strong></p>
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            <ol>
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              <li>Add 2ml of the inoculum in 100ml of liquid LB medium. Incubate at 37°C/250rpm until the OD reaches 0,375</li>
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              <li> Distribute the volume in 2 250ml centrifuge tubes (pre chilled) and spin at 10000rpm/7min/4°C</li>
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              <li> Discard the supernatant and ressuspend the pellet in 10ml of CaCl<sub>2</sub> solution</li>
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              <li> Spin the tubes at 7000rpm/7min/4°C</li>
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              <li> Discard the supernatant and ressuspend the pellet in 10ml of CaCl<sub>2</sub> solution. Incubate 30min on ice</li>
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              <li> Spin at 7000rpm/7min/4°C</li>
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              <li> Discard the supernatant and ressuspend the pellet in 2ml of the CaCl<sub>2</sub> solution</li>
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              <li> Distribute the 2ml in 500ul tubes adding 50ul in each.</li>
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              <li> Freeze the samples in liquid nitrogen </li>
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              <li> Stock at -80°C </li>
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            </ol>
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      </ol>
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<h2> Transformation in <em>Escherichia coli</em> (DH5-alpha)</h2>
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  <ol>
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      <li><h3> Materials</h3></li>
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        <ul>
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            <li> 1,5ml tube</li>
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            <li> Styrofoam box with ice</li>
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            <li> Agar plate with antibiotic </li>
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            <li> Competent cells </li>
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            <li> Plasmidial DNA </li>
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            <li> Centrifuge </li>
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            <li> Water bath at 42°C </li>
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            <li> Liquid LB medium </li>       
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            <li> Shaker </li>
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        </ul>
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      <li><h3>Methods</h3></li>
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        <ol>
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            <li>Briefly spin the competent cells and put then on ice </li>
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            <li> Add 50ng of plasmidial DNA in a 1,5ml tube </li>
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            <li> Add the 50ul of competent cell in the same tube</li>
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            <li> Keep the tube on ice for 25min </li>
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            <li> Put the tube in a 42° water bath for 2 min</li>
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            <li> Put the tube on ice for 5 min</li>
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            <li> Add 200ul of liquid LB </li>
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            <li> Incubate at 250rpm/37°C/1 hour </li>
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            <li> Plate the the solution in a Agar plate with the appropriate antibiotic </li>
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            <li> Incubate the plate at 37°C overnight </li>
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        </ol>
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  </ol>
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<h1>Assemblies</h1>
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<!--estou colocando os fichamentos dos assemblies aqui, ainda preciso acabar os que faltam mas ja da pra experimentar com a organização destes-->
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  <ul>
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      <li><a href="https://static.igem.org/mediawiki/2014/e/eb/Assembly_Form.pdf">Assembly Form</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a></li
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      <li><a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a></li>
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  </ul>
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<h1><strong>Life Inside the LAB</stron></h1>
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<thead><h2>July</h2>
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<thead><h2 align=center>July</h2>
</thead>
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     <th>Monday</th>  
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     <th style="background-color:#bfacc0">Monday</th>  
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     <th>Tuesday</th>
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     <th style="background-color:#bfacc0">Tuesday</th>
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     <th>Wednesday</th>
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     <th style="background-color:#bfacc0">Wednesday</th>
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     <th>Thursday</th>
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     <th style="background-color:#bfacc0">Thursday</th>
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     <th>Friday</th>
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     <th style="background-color:#bfacc0">Friday</th>
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     <th>Saturday</th>
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     <th style="background-color:#bfacc0">Saturday</th>
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     <th>Sunday</th>
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     <th style="background-color:#bfacc0">Sunday</th>
   </tr> <tr>
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     <td>30/06
     <td>30/06
     <ul>
     <ul>
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       <li>PCR lasR (BBa_C0079) for amplification + RBS addition using primers</li>
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       <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> lasR (<a href="http://parts.igem.org/Part:BBa_C0079" style="color:#3ab473">BBa_C0079</a>). The purpose of this PCR was to add the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021</a>), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called <a href="http://parts.igem.org/Part:BBa_K1521001" style="color:#3ab473">BBa_K1521001</a>.</li>
     </ul>
     </ul>
     </td>
     </td>
     <td>01/07  
     <td>01/07  
     <ul>
     <ul>
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       <li>Purification of lasR PCR product </li>
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       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> of lasR <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> product </li>
     </ul>
     </ul>
     </td>
     </td>
     <td>02/07
     <td>02/07
     <ul>
     <ul>
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       <li>Transformation of the Biobricks:</li>
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       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks:</li>
       <ul>
       <ul>
-
         <li>BBa_143012</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
-
         <li>BBa_K081001</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
-
         <li>BBa_E0840</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
       </ul>
       </ul>
       <li>Inoculum of the Biobricks sent by iGEM HQ:</li>
       <li>Inoculum of the Biobricks sent by iGEM HQ:</li>
       <ul>
       <ul>
-
         <li>BBa_K316037</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
-
         <li>BBa_K316018</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li>
-
         <li>BBa_K316015</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 279: Line 153:
     <td>03/07
     <td>03/07
     <ul>
     <ul>
-
       <li>Transformation of the Biobricks sent by the Imperial College Team:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks sent by the Imperial College Team:</li>
       <ul>
       <ul>
-
         <li>BBa_K316016</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016<a/></li>
-
         <li>BBa_K143031</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143031" style="color:#3ab473">BBa_K143031<a/></li>
       </ul>
       </ul>
-
       <li>Miniprep, Quantification of the plasmidial DNA samples and Restriction Analysis:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification of the plasmidial DNA samples and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_143012</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
-
         <li>BBa_K081001</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li>
-
         <li>BBa_E0840</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 298: Line 172:
       <li>Inoculum:</li>
       <li>Inoculum:</li>
       <ul>
       <ul>
-
         <li>BBa_143012</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
-
         <li>BBa_K081001</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
-
         <li>BBa_E0840</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 308: Line 182:
     <td>07/07
     <td>07/07
     <ul>
     <ul>
-
       <li>Miniprep, Quantification and Restriction Analysis:
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_143012</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
-
         <li>BBa_K081001</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
-
         <li>BBa_E0840</li>
+
         <li<a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
       </ul>
       </ul>
-
       <li>Transformation:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_B0015</li>
+
         <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 325: Line 199:
     <li>Inoculum:</li>
     <li>Inoculum:</li>
       <ul>
       <ul>
-
         <li>BBa_B0015</li>
+
         <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 331: Line 205:
     <td>10/07
     <td>10/07
     <ul>
     <ul>
-
       <li>Miniprep, Quantification and Restriction Analysis:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_B0015 (restriction analysis fail, repeat)</li>
+
         <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a> (restriction analysis fail, repeat)</li>
       </ul>
       </ul>
-
       <li>PCR qteE for amplification + RBS addition using primers</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> the qteE gene for amplification. We also added the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021,</a>) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called <a href="http://parts.igem.org/Part:BBa_K1521000" style="color:#3ab473">BBa_K1521000</a>.</li>
-
       <li>Ligation of RBS+lasR in PTZ57R/T vector (instaclone kit)</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of RBS+lasR in PTZ57R/T vector (instaclone kit)</li>
     </ul>
     </ul>
     </td>
     </td>
Line 342: Line 216:
     <ul>
     <ul>
       <li>Prepare 40% glycerol solution</li>
       <li>Prepare 40% glycerol solution</li>
-
       <li>Transformation:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
       <ul>
       <ul>
-
         <li>RBS + lasR (BBa_C0079)</li>
+
         <li>RBS + lasR (<a href="http://parts.igem.org/Part:BBa_C0079"style="color:#3ab473">BBa_C0079</a>)</li>
       </ul>
       </ul>
-
       <li>Purification:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a>:</li>
       <ul>
       <ul>
         <li>RBS + qteE</li>
         <li>RBS + qteE</li>
Line 359: Line 233:
       <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li>
       <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li>
       <ul>
       <ul>
-
         <li>Digestion EXSP</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
       </ul>
       </ul>
       <li>Prepare X-gal</li>
       <li>Prepare X-gal</li>
Line 372: Line 246:
       </ul>
       </ul>
       <li>Replate RBS+lasR</li>
       <li>Replate RBS+lasR</li>
-
       <li>Ligation of PCR qteE in pGEM T-easy vector</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> qteE in pGEM T-easy vector</li>
-
       <li>Transformation:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_J04450</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> <a href="" style="color:#3ab473"> BBa_J04450</a></a></li>
       </ul>
       </ul>
-
       <li>Restriction analysis:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_B0015</li>
+
         <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 385: Line 259:
     <td>16/07
     <td>16/07
     <ul>
     <ul>
-
     <li>Tranformation:</li>
+
     <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a>:</li>
       <ul>
       <ul>
-
       <li>BBa_K823003</li>
+
       <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li>
       <li>RBS+qteE</li>
       <li>RBS+qteE</li>
       </ul>
       </ul>
     <li>Electrophoresis analysis:</li>
     <li>Electrophoresis analysis:</li>
       <ul>
       <ul>
-
       <li>BBa_B0015</li>
+
       <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 398: Line 272:
     <td>17/07
     <td>17/07
     <ul>
     <ul>
-
       <li>Glycerol Stock and Miniprep:<li>
+
       <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:<li>
       <ul>
       <ul>
-
         <li>BBa_J04450</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
         <li>RBS+lasR</li>
         <li>RBS+lasR</li>
-
         <li>BBa_B0015</li>
+
         <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
       </ul>
       </ul>
-
       <li>Digestion:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_J04450 (EP)</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li>
-
         <li>BBa_J04450 (XS)</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li>
       </ul>
       </ul>
       <li>Inoculum:</li>
       <li>Inoculum:</li>
       <ul>
       <ul>
-
         <li>BBa_K823003</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li>
       </ul>
       </ul>
-
       <li>Transformation</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       <ul>
       <ul>
-
         <li>BBa_K143055</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 421: Line 295:
     <td>18/07
     <td>18/07
     <ul>
     <ul>
-
       <li>Glycerol Stock and Miniprep:</li>
+
       <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_J04450</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
-
         <li>BBa_B0015</li>
+
         <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
       </ul>
       </ul>
-
       <li>Gel Purification:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_J04450 (EP)</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li>
-
         <li>BBa_J04450 (XS)</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li>
       </ul>
       </ul>
-
       <li>Restriction analysis:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_K823003</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 442: Line 316:
     <td>21/07
     <td>21/07
     <ul>
     <ul>
-
       <li>Transformation:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_316016</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
       </ul>
       </ul>
       <li>Inoculum:</li>
       <li>Inoculum:</li>
       <ul>
       <ul>
-
         <li>BBa_K143055</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
         <li>RBS+qteE</li>
         <li>RBS+qteE</li>
       </ul>
       </ul>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
       <ul>
       <ul>
-
         <li>Digestion EXSP</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 461: Line 335:
       <li>Inoculum:</li>
       <li>Inoculum:</li>
       <ul>
       <ul>
-
         <li>BBa_K316016</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
       </ul>
       </ul>
-
       <li>Miniprep, Restriction analysis and Digestion:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
       <ul>
       <ul>
         <li>RBS+qteE</li>
         <li>RBS+qteE</li>
Line 469: Line 343:
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
       <ul>
       <ul>
-
         <li>Gel Purification</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
-
         <li>Ligation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 478: Line 352:
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
       <ul>
       <ul>
-
         <li>Transformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       </ul>
       </ul>
-
       <li>Prepare more competent cells</li>
+
       <li>Prepare more <a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf" style="color:#f05151"> competent cells</a></li>
       <li>Prepare LB medium (solid and liquid)</li>
       <li>Prepare LB medium (solid and liquid)</li>
       <li>Inoculum</li>
       <li>Inoculum</li>
       <ul>
       <ul>
-
         <li>BBa_K316016</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
       </ul>
       </ul>
       <li>Prepare RBS+lasR and RBS+qteE for sequencing</li>
       <li>Prepare RBS+lasR and RBS+qteE for sequencing</li>
Line 495: Line 369:
         <li>Inoculum</li>
         <li>Inoculum</li>
       </ul>
       </ul>
-
       <li>Miniprep:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_K316016</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 506: Line 380:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
-
         <li>Restriction analysis</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 518: Line 392:
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>:</li>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>:</li>
       <ul>
       <ul>
-
         <li>Restriction analysis (+NdeI enzyme)</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (+NdeI enzyme)</li>
-
         <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that bot the construction and the pSB1C3 vector had similar size. Check out the Lab Journal report of the day for more information.</li>
+
         <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.</li>
       </ul>
       </ul>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
Line 525: Line 399:
           <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
       <ul>
       <ul>
-
         <li>Digestion EXSP</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
       </ul>  
       </ul>  
     </ul>
     </ul>
Line 535: Line 409:
           <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
       <ul>
       <ul>
-
         <li>Gel Purification</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
-
         <li>Ligation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 546: Line 420:
           <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
       <ul>
       <ul>
-
         <li>Transformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       </ul>
       </ul>
     </ul>     
     </ul>     
Line 558: Line 432:
         <li>Inoculum</li>
         <li>Inoculum</li>
       </ul>
       </ul>
-
       <li>PCR BBa_K143055 for RBS removal</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for RBS removal</li>
       <li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this  Lac promotor :) </li>
       <li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this  Lac promotor :) </li>
-
       <li>PCR of Plac BBa_K143055 was followed by a Gel Purification and Ligation in the PUC19 vector</li>
+
       <li>PCR of Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> was followed by a <a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> in the PUC19 vector</li>
     </ul>
     </ul>
     </td>
     </td>
Line 568: Line 442:
   </tr>
   </tr>
</table>
</table>
 +
<br>
 +
<br>
 +
<br>
 +
 +
</tbody>
</tbody>
-
<thead><h2>August</h2>
+
<thead><h2 align=center>August</h2>
</thead>
</thead>
Line 579: Line 458:
<table style="width:100%">
<table style="width:100%">
   <tr>
   <tr>
-
     <th>Monday</th>
+
     <th style="background-color:#bfacc0">Monday</th>  
-
     <th>Tuesday</th>
+
     <th style="background-color:#bfacc0">Tuesday</th>
-
     <th>Wednesday</th>
+
     <th style="background-color:#bfacc0">Wednesday</th>
-
     <th>Thursday</th>
+
     <th style="background-color:#bfacc0">Thursday</th>
-
     <th>Friday</th>
+
     <th style="background-color:#bfacc0">Friday</th>
-
     <th>Saturday</th>
+
     <th style="background-color:#bfacc0">Saturday</th>
-
     <th>Sunday</th>
+
     <th style="background-color:#bfacc0">Sunday</th>
   </tr> <tr>
   </tr> <tr>
     <td></td>
     <td></td>
Line 593: Line 472:
     <td>01/08
     <td>01/08
     <ul>
     <ul>
-
       <li>Transformation:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_K143055</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
       </ul>
       </ul>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
Line 602: Line 481:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
-
         <li>Restriction analysis</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 617: Line 496:
         <li>E. coli DH5-alpha</li>
         <li>E. coli DH5-alpha</li>
         <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li>
-
         <li>Plac BBa_K143055</li>
+
         <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
       </ul>
       </ul>
-
       <li>Transformation</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       <ul>
       <ul>
-
         <li>BBa_P0312</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
       </ul>
       </ul>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
       <ul>
       <ul>
         <li>Prepare more inoculum</li>
         <li>Prepare more inoculum</li>
-
         <li>Repeat Restriction analysis (for more info go to the day's report)</li>
+
         <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 633: Line 512:
     <ul>
     <ul>
       <li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li>
       <li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li>
-
       <li>Miniprep:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
       <ul>
       <ul>
-
         <li>Plac BBa_K143055</li>
+
         <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
       </ul>
       </ul>
       <li>Inoculum</li>
       <li>Inoculum</li>
       <ul>
       <ul>
-
         <li>BBa_P0312</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
       </ul>
       </ul>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
       <ul>
       <ul>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
-
         <li>And once again! Restriction analysis (this BBa_K316016 is tough)</li>
+
         <li>And once again! <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (this <a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a> is tough)</li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 651: Line 530:
     <td>07/08
     <td>07/08
     <ul>
     <ul>
-
       <li>MIniprep and Restriction analysis:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_P0312</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 663: Line 542:
     <td>11/08
     <td>11/08
     <ul>
     <ul>
-
       <li>Prepare Plac BBa_K143055 for sequencing</li>
+
       <li>Prepare Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for sequencing</li>
       <li>Analyse the RBS+qteE and RBS+lasR sequencing file</li>
       <li>Analyse the RBS+qteE and RBS+lasR sequencing file</li>
     </ul>
     </ul>
Line 669: Line 548:
     <td>12/08
     <td>12/08
     <ul>
     <ul>
-
       <li>Digestion:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
       <ul>
       <ul>
         <li>RBS+qteE</li>
         <li>RBS+qteE</li>
Line 682: Line 561:
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
       <ul>
       <ul>
-
         <li>Digestion EXSP</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 688: Line 567:
     <td>14/08
     <td>14/08
     <ul>
     <ul>
-
       <li>Gel Purification and Ligation in pSB1C3 for BBiobrick standard:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a> in pSB1C3 for BBiobrick standard:</li>
       <ul>
       <ul>
         <li>RBS+qteE</li>
         <li>RBS+qteE</li>
Line 695: Line 574:
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and  
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and  
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
-
       <li>Gel Purification</li>
+
       <ul>
-
      <li>OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion</li>
+
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li>OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion</li>
 +
      </ul>
     </ul>
     </ul>
     </td>
     </td>
Line 705: Line 586:
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
       <ul>
       <ul>
-
         <li>Digestion EXSP</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 714: Line 595:
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
       <ul>
       <ul>
-
         <li>Gel Purification</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 724: Line 605:
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
       <ul>
       <ul>
-
         <li>Ligation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
       </ul>
       </ul>
       <li>Prepare LB medium (liquid and solid)</li>
       <li>Prepare LB medium (liquid and solid)</li>
Line 734: Line 615:
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
       <ul>
       <ul>
-
         <li>Transformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 753: Line 634:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a></li>
-
         <li>Restriction analysis</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 763: Line 644:
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li>
       <ul>
       <ul>
-
         <li>Repeat restriction analysis adding the enzyme NdeI (for more info check out the Lab Journal)</li>
+
         <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">restriction analysis</a> adding the enzyme NdeI.</li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 774: Line 655:
       <li>Inoculum</li>
       <li>Inoculum</li>
       <ul>
       <ul>
-
         <li>BBa_J04450</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
-
         <li>BBa_K143055</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
       </ul>
       </ul>
-
       <li>PCR BBa_K143055 with higher melting temperature (check out our Lab Journal for more info)</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> with higher melting temperature.</li>
     <ul>
     <ul>
     </td>
     </td>
Line 788: Line 669:
         <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
         <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
       <ul>
       <ul>
-
         <li>Digestion EXSP</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 800: Line 681:
         <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
         <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
       <ul>
       <ul>
-
         <li>Gel Purification</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 810: Line 691:
   </tr>
   </tr>
</table>
</table>
 +
<br>
 +
<br>
 +
<br>
-
<thead><h2>September</h2>
+
<thead><h2 align=center>September</h2>
</thead>
</thead>
Line 819: Line 703:
<table style="width:100%">
<table style="width:100%">
   <tr>
   <tr>
-
     <td>Monday</td>
+
     <th style="background-color:#bfacc0">Monday</th>  
-
     <td>Tuesday</td>
+
     <th style="background-color:#bfacc0">Tuesday</th>
-
     <td>Wednesday</td>
+
     <th style="background-color:#bfacc0">Wednesday</th>
-
     <td>Thursday</td>
+
     <th style="background-color:#bfacc0">Thursday</th>
-
     <td>Friday</td>
+
     <th style="background-color:#bfacc0">Friday</th>
-
     <td>Saturday</td>
+
     <th style="background-color:#bfacc0">Saturday</th>
-
     <td>Sunday</td>
+
     <th style="background-color:#bfacc0">Sunday</th>
   </tr> <tr>
   </tr> <tr>
     <td>01/09
     <td>01/09
Line 831: Line 715:
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>
       <ul>
       <ul>
-
         <li>Digestion of the part BII with EcoRV (for more info check out our Lab Journal)
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a> of the part BII with EcoRV.
       </ul>
       </ul>
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,  
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,  
Line 839: Line 723:
         <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
         <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
       <ul>
       <ul>
-
         <li>Ligation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 851: Line 735:
         <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
         <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
       <ul>
       <ul>
-
         <li>Transformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 876: Line 760:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
-
         <li>Restriction analysis</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 888: Line 772:
     <td>08/09
     <td>08/09
     <ul>
     <ul>
-
       <li>QuickChange PCR to mutate the cleavage site of the BBa_316037 to a Cathepsin S recognition site</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li>
-
       <li>Transformation of the QuickCHange PCR product</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li>
     </ul>
     </ul>
     </td>
     </td>
Line 896: Line 780:
     <ul>
     <ul>
       <li>The QuickChange protocol didn't work well. So try again with more attention!</li>
       <li>The QuickChange protocol didn't work well. So try again with more attention!</li>
-
       <li>QuickChange PCR to mutate the cleavage site of the BBa_316037 to a Cathepsin S recognition site</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li>
-
       <li>Transformation of the QuickCHange PCR product</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li>
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
           <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,  
           <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,  
Line 903: Line 787:
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
       <ul>
       <ul>
-
         <li>Digestion EXSP</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
       </ul>  
       </ul>  
     </ul>
     </ul>
Line 909: Line 793:
     <td>11/09
     <td>11/09
     <ul>
     <ul>
-
       <li>Prepare inoculum of the mutated BBa_K316037</li>
+
       <li>Prepare inoculum of the mutated <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
           <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,  
           <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,  
Line 915: Line 799:
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
       <ul>
       <ul>
-
         <li>Gel Purification</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
-
         <li>Ligation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
       </ul>   
       </ul>   
     </ul>
     </ul>
Line 927: Line 811:
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
       <ul>
       <ul>
-
         <li>Tranformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a></li>
       </ul>
       </ul>
-
       <li>We had far to many difficulties trying to remove the RBS from the BBa_K143055, so we decided to synthesize it using PCR.</li>
+
       <li>We had far to many difficulties trying to remove the RBS from the <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a>, so we decided to synthesize it using PCR.</li>
-
       <li>PCR for BBa_143015 (BBa_143055 without RBS)</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf" style="color:#f05151">PCR for synthesis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> (<a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> without RBS)</li>
     </ul>
     </ul>
     </td>
     </td>
Line 943: Line 827:
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
       <ul>
       <ul>
-
         <li>Repeat Ligation</li>
+
         <li>Repeat <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
       </ul>
       </ul>
       <li>Prepare LB medium (solid and liquid)</li>
       <li>Prepare LB medium (solid and liquid)</li>
Line 953: Line 837:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
       </ul>
       </ul>
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
Line 959: Line 843:
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
       <ul>
       <ul>
-
         <li>Transformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 968: Line 852:
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>  
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>  
       <ul>
       <ul>
-
         <li>Restriction analysis</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
Line 976: Line 860:
         <li>Inoculum</li>
         <li>Inoculum</li>
       </ul>
       </ul>
-
       <li>Gel Purification of the BBa_K143015</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
-
     </ul>
+
     </ul>    
     </td>
     </td>
     <td>16/09
     <td>16/09
Line 987: Line 871:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
-
         <li>Restriction analysis</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 994: Line 878:
     <td>17/09
     <td>17/09
     <ul>
     <ul>
-
       <li>LIgation of the synthesized BBa_K143015 in TOPO vector followed by Transformation</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of the synthesized <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> in TOPO vector followed by <a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
     </ul>
     </ul>
 +
 +
    <ul>
 +
      <li>Measurement Interlab Study - Samples growth biobrick devices 1, 2 and 3</li>
 +
    </ul>
 +
   
     </td>
     </td>
     <td>18/09
     <td>18/09
     <ul>
     <ul>
-
       <li>Replate BBa_K143015. We forgot the X-gal :(</li>
+
       <li>Replate <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a>. We forgot the X-gal :(</li>
 +
    </ul>
 +
    <ul>
 +
      <li>Measurement Interlab Study - Sample preparation and characterization by Flow Cytometry and Fluorometry</li>
     </ul>
     </ul>
     </td>
     </td>
Line 1,008: Line 900:
     <td>22/09
     <td>22/09
     <ul>
     <ul>
-
       <li>Inoculum of the BBa_K143015</li>
+
       <li>Inoculum of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
-
       <li>Assembly KXIV
+
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
     </ul>
     </ul>
 +
   
     </td>
     </td>
-
     <td>23/09</td>
+
     <td>23/09
-
     <td>24/09</td>
+
    <ul>
-
     <td>25/09</td>
+
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
-
     <td>26/09</td>
+
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
 +
    </ul>
 +
    </td>
 +
     <td>24/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
     <td>25/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
     <td>26/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
     <td>27/09</td>
     <td>27/09</td>
     <td>28/09</td>
     <td>28/09</td>
   </tr> <tr>
   </tr> <tr>
-
     <td>29/09</td>
+
     <td>29/09
-
     <td>30/09</td>
+
    <ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
     <td>30/09
 +
    <ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
     <td></td>
     <td></td>
     <td></td>
     <td></td>
Line 1,028: Line 983:
   </tr>
   </tr>
</table>
</table>
 +
<br>
 +
<br>
 +
<br>
 +
 +
-
<thead><h2>October</h2>
+
<thead><h2 align=center>October</h2>
</thead>
</thead>
Line 1,037: Line 997:
<table style="width:100%">
<table style="width:100%">
   <tr>
   <tr>
-
     <td>Monday</td>
+
     <th style="background-color:#bfacc0">Monday</th>  
-
     <td>Tuesday</td>
+
     <th style="background-color:#bfacc0">Tuesday</th>
-
     <td>Wednesday</td>
+
     <th style="background-color:#bfacc0">Wednesday</th>
-
     <td>Thursday</td>
+
     <th style="background-color:#bfacc0">Thursday</th>
-
     <td>Friday</td>
+
     <th style="background-color:#bfacc0">Friday</th>
-
     <td>Saturday</td>
+
     <th style="background-color:#bfacc0">Saturday</th>
-
     <td>Sunday</td>
+
     <th style="background-color:#bfacc0">Sunday</th>
   </tr> <tr>
   </tr> <tr>
     <td></td>
     <td></td>
-
     <td>30/07</td>
+
     <td></td>
-
     <td>01/08</td>
+
     <td>01/10
-
     <td>02/08</td>
+
    <ul>
-
     <td>03/08</td>
+
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
-
     <td>Points</td>
+
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
-
     <td>Points</td>
+
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
     <td>02/10</td>
 +
     <td>03/10</td>
 +
     <td>04/10</td>
 +
     <td>05/10</td>
   </tr>
   </tr>
  <tr>
  <tr>
-
     <th>First Name</th>
+
     <td>06/10
-
     <th>Last Name</th>
+
    <ul>
-
     <th>Points</th>
+
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
-
     <th>Points</th>
+
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
-
     <th>Points</th>
+
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
-
     <th>Points</th>
+
      <ul>
-
     <th>Points</th>
+
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <ahref="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
     </td>
 +
    <td>07/10 
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
     <td>08/10
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    <ul>
 +
     </td>
 +
    <td>09/10
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
     </td>
 +
    <td>10/10
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
     </td>
 +
    <td>11/10</td>
 +
     <td>12/10</td>
   </tr> <tr>
   </tr> <tr>
-
     <th>First Name</th>
+
     <td>13/10</td>
-
     <th>Last Name</th>
+
     <td>14/10</td>
-
     <th>Points</th>
+
     <td>15/10</td>
-
     <th>Points</th>
+
     <td>16/10</td>
-
     <th>Points</th>
+
     <td>17/10</td>
-
     <th>Points</th>
+
     <td>18/10</td>
-
     <th>Points</th>
+
     <td>19/10</td>
   </tr> <tr>
   </tr> <tr>
-
     <th>First Name</th>
+
     <td>20/10</td>
-
     <th>Last Name</th>
+
     <td>21/10</td>
-
     <th>Points</th>
+
     <td>22/10</td>
-
     <th>Points</th>
+
     <td>23/10</td>
-
     <th>Points</th>
+
     <td>24/10</td>
-
     <th>Points</th>
+
     <td>25/10</td>
-
     <th>Points</th>
+
     <td>26/10</td>
   </tr> <tr>
   </tr> <tr>
-
     <th>First Name</th>
+
     <td>27/10</td>
-
     <th>Last Name</th>
+
     <td>28/10</td>
-
     <th>Points</th>
+
     <td>29/10</td>
-
     <th>Points</th>
+
     <td>30/10</td>
-
     <th>Points</th>
+
     <td>31/10</td>
-
     <th>Points</th>
+
     <td></td>
-
     <th>Points</th>
+
     <td></td>
   </tr>
   </tr>
</table>
</table>
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{{:Team:Brasil-SP/Templates/Footer}}

Latest revision as of 20:32, 17 October 2014

HeaderNotebookBRASILAP.png

Main Assembly Map

     The main assembly map describes all the constructions that would be done in the project, but some of them have not been performed in time of the iGEM deadline. The succesfully constructed assemblies is indicated by a green check point and the unsuccessfull constructions are indicated by a red "X". Click on these assemblies to access the lab form.

AI AII AIII BIII AIV BII CII DI AVI AV BIV

Assemblies forms

Assembly form template

This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)

Life Inside the LAB

July

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
30/06
  • PCR lasR (BBa_C0079). The purpose of this PCR was to add the RBS (BBa_K143021), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called BBa_K1521001.
 01/07 02/07 03/07 04/07 05/07 06/07
07/07 08/07 09/07 10/07
  • Miniprep, Quantification and Restriction Analysis:
    • BBa_B0015 (restriction analysis fail, repeat)
  • PCR the qteE gene for amplification. We also added the RBS (BBa_K143021,) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called BBa_K1521000.
  • Ligation of RBS+lasR in PTZ57R/T vector (instaclone kit)
 11/07 12/07 13/07
14/07 15/07 16/07 17/07 18/07 19/07 20/07
21/07 22/07 23/07 24/07 25/07 26/07 27/07
28/07
  • Assembly AIII:
    • Restriction analysis (+NdeI enzyme)
    • OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.
  • Assembly AII, AV and AVI:
 29/07 30/07 31/07
  • Assembly AII, AV and AVI:
    • Inoculum
  • PCR BBa_K143055 for RBS removal
  • OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :)
  • PCR of Plac BBa_K143055 was followed by a Gel Purification and Ligation in the PUC19 vector



August

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/08 02/08 03/08
04/08 05//08 06/08 07/08 08/08 09/08 10/08
11/08
  • Prepare Plac BBa_K143055 for sequencing
  • Analyse the RBS+qteE and RBS+lasR sequencing file
 12/08
  • Digestion:
    • RBS+qteE
    • RBS+lasR
    • OBS: this digestion was performed so we clone this parts in the pSB1C3 vector to put them in Biobrick standard
 13/08 14/08
  • Gel Purification and Ligation in pSB1C3 for BBiobrick standard:
    • RBS+qteE
    • RBS+lasR
  • Assemblies BII and BIII:
    • Gel Purification
    • OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion
 15/08 16/08 17/08
18/08 19/08 20/08
  • RBS+qteE, RBS+lasR and Assemblies BII and BIII:
    • Inoculum
 21/08 22/08 23/08 24/08
25/08 26/08 27/08 28/08 29/08 30/08 31/08



September

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/09 02/09 03/09 04/09 05/09 06/09 07/09
08/09 09/09 10/09 11/09 12/09 13/09
  • Assembly KX
    • Inoculum
  • Assemblies DI, KVIII and KXI:
  • Prepare LB medium (solid and liquid)
 14/09
15/09 16/09 17/09
  • Measurement Interlab Study - Samples growth biobrick devices 1, 2 and 3
 18/09
  • Measurement Interlab Study - Sample preparation and characterization by Flow Cytometry and Fluorometry
 19/09 20/09 21/09
22/09 23/09 24/09 25/09
  • Assembly KXIV:
    • Inoculum
 26/09 27/09 28/09
29/09 30/09



October

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/10 02/10 03/10 04/10 05/10
06/10 07/10 08/10 09/10
  • Assemblies KIX, BIV and KXVI
    • Inoculum
    		 10/10 11/10 12/10
    13/10 14/10 15/10 16/10 17/10 18/10 19/10
    20/10 21/10 22/10 23/10 24/10 25/10 26/10
    27/10 28/10 29/10 30/10 31/10

    Sponsors

    This project is part of iGEM 2014

    Wiki Links

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    Retrieved from "http://2014.igem.org/Team:Brasil-SP/Notebook"