Latest revision as of 20:25, 17 October 2014

BUCT-CHINA

PROJECT

Device

This device includes two parts, one of them is physical instrument and the other is matched software.

Our device matched experimental detection is consists of fourteen parts, including bottom plat, guide rail, chip slot, motor cabinet, toothed belt, driving wheel, driven wheel, testing scaffold, LED support, control, light barrier, hemispheroid, miniature photoelectric switch, article shading, etc.

The graphical representation will clarify the principle of how this device works.

Step1: Bioluminescence, mostly depends on the reporter genes varies as gfp, rfp, yfp etc;

Step2:Collecting lights: in this part, we use a photo dyed(PD) to collect light intensity data then transform it into electricity (nA). Here we need a illuminant to motivate the chassis bacteria to produce bioluminescence in our comprehensive detection in E.coli; because our recombinant bioluminescent E.coli is transmitted into a plasmid with luxAB to produce blue-green light itself so there is no need to add an outer illuminant. On the contrary, as for another specific detection, an additional illuminant is necessary. It is worth noting that we use LED (385nm) as stimulant. Consequently, the following circuits will transform electricity into voltage for the range of 0-10V.

Step3：Operational amplifier. It is an OP-AMP precision and the model is OPA602AU.

Step4: Second-order active low-pass filter. It will complete signal processing .

Step5: Analog digital conversion(ADC),type is AD7707. Through this process, the analog signal will exist in a digital way.

Step6: Single Chip Microcomputer. Digital signal will come into play. It will be linked to a computer by serial ports.

Figure: The structure of matched chips which are used in our device.

Figure:The software we designed to match with our device .

The device has been tested using rhodamine B. Following figure is the sensitivity curve. The detection limit is lower than 1ng/ml.

Figure:Sensitivity curve

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 		  		<h3>PROJECT</h3>
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-                                  <h1 style="font-size:44px; top:30px; text-align: center;"><b>device</b></h1>
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+                               <p style="text-align:center; color:#333; font-size:20px"><img src="https://static.igem.org/mediawiki/2014/4/4f/All.png" alt="" align="absmiddle" /></p>
-                                 <p style="text-align:left; color:#333; font-size:20px">There are a population of 884 million still drink water without the previous purification all over the world. There are about 3 billion to 4 billion families lack of drinkable water. Each year about 3.5 million people's deaths are associated with insufficient water supply and sanitation condition, which occurs mainly in developing countries.</p><br>
+                                 <p style="text-align:left; color:#333; font-size:20px">This device includes two parts, one of them is physical instrument and the other is matched software.</p>
+ <p style="text-align:left; color:#333; font-size:20px">Our device matched experimental detection is consists of fourteen parts, including bottom plat, guide rail, chip slot, motor cabinet, toothed belt, driving wheel, driven wheel, testing scaffold, LED support, control, light barrier, hemispheroid, miniature photoelectric switch, article shading, etc. </p>
-<p style="text-align:left; color:#333; font-size:20px">According to all above, the matter of water quality is a great matter of human beings’ life quality. It has always been crucial to many researchers engaging in improving and making breakthroughs on the approaches towards water quality detection. Currently chemical detection methods are more complex and time consuming; besides ,on the way of bringing the samples to the laboratory, some of the indicators may probably be changed. Another thing we may acknowledge is that chemical detection apparatus cost too much money.  </p><br>
+<p style="text-align:left; color:#333; font-size:20px">The graphical representation will clarify the principle of how this device works. </p>
+<p style="text-align:center; color:#333; font-size:20px"><img src="https://static.igem.org/mediawiki/2014/a/a4/Device.png" alt="" align="absmiddle" /></p>
-<p style="text-align:left; color:#333; font-size:20px">Based on the matters above, this year we intended to look for a new way using biotechnology. Here come the ideas, through two kinds of approaches we may achieve rapid water quality detection. One of the approaches is so called comprehensive detection, here we mainly rely on LuxAB ,which encodes a kind of luciferase. Because anything toxic within the water may have a negative impact on the activity of luciferase, the luminescence is likely to be decreased. The other is specific detection, because only in this way can we successfully quantify specific metal ions like Hg(II). One sequence of MerR operon occupies a significant position along with quorum sensing system. In addition, we use gfp as reporter.</p><br>
+<p style="text-align:left; color:#333; font-size:20px">Step1: Bioluminescence, mostly depends on the reporter genes varies as gfp, rfp, yfp etc;</p>
-<p style="text-align:left; color:#333; font-size:20px">Besides the two biosensors we built, the joint uses of microfluidic chips, automatic detection devices, photoelectric detection technology and micro total analysis systems will extremely promote biological detection method. This device can also be linked to the computer and has the ability of offline CNC screen operation, which improves the detection efficiency. What’s more, little device is convenient to carry and free from  limitation of experimental conditions.</p><br><br>
+<p style="text-align:left; color:#333; font-size:20px">Step2:Collecting lights: in this part, we use a photo dyed(PD) to collect light intensity data then transform it into electricity (nA). Here we need a illuminant to motivate the chassis bacteria to produce bioluminescence in our comprehensive detection in E.coli; because our recombinant bioluminescent E.coli is transmitted into a plasmid with luxAB to produce blue-green light itself so there is no need to add an outer illuminant. On the contrary, as for another specific detection, an additional illuminant is necessary. It is worth noting that we use LED (385nm) as stimulant. Consequently, the following circuits will transform electricity into voltage for the range of 0-10V. </p>
-<p style="text-align:left; color:#333; font-size:20px">Biological method not only has the priority of concise operations and less consumption, but also has the advantages of high accuracy, specificity, and security.</p><br><br>
+<p style="text-align:left; color:#333; font-size:20px">Step3：Operational amplifier. It is an OP-AMP precision and the model is OPA602AU.</p>
+<p style="text-align:left; color:#333; font-size:20px">Step4: Second-order active low-pass filter. It will complete signal processing .</p>
+<p style="text-align:left; color:#333; font-size:20px">Step5: Analog digital conversion(ADC),type is AD7707. Through this process, the analog signal will exist in a digital way.</p>
+<p style="text-align:left; color:#333; font-size:20px">Step6: Single Chip Microcomputer. Digital signal will come into play. It will be linked to a computer by serial ports. </p>
+<p style="text-align:center; color:#333; font-size:20px"><img src="https://static.igem.org/mediawiki/2014/8/84/Xinp.jpg" alt="" align="absmiddle" /></p>
+<p style="color:#333; font-size:15px; italic">Figure: The structure of matched chips which are used in our device.   </p>
+<p style="text-align:center; color:#333; font-size:20px"><img src="https://static.igem.org/mediawiki/2014/1/14/Softw.jpg" alt="" align="absmiddle" /></p>
+<p style="color:#333; font-size:15px; italic">Figure:The software we designed to match with our device .        </p>
+<p style="text-align:left; color:#333; font-size:20px">The device has been tested using rhodamine B. Following figure is the sensitivity curve. The detection limit is lower than 1ng/ml.
 </p>
+<p style="text-align:center; color:#333; font-size:20px"><img src="https://static.igem.org/mediawiki/2014/3/37/Shiyan.jpg" alt="" align="absmiddle" /></p>
+<p style="text-align:center; color:#333; font-size:20px"><img src="https://static.igem.org/mediawiki/2014/1/11/Xiaxian.JPG" alt="" align="absmiddle" /></p>
+<p style="color:#333; font-size:15px; italic">Figure:Sensitivity curve </p>
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Latest revision as of 20:25, 17 October 2014

PROJECT

Device