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Team:Evry/Notebook/Transposons/09.01.14
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| - | The product of PCR is purified with dialyse during 2h. | + | <h4> Purification of pNK2 with Ori muted </h4><p> |
| + | The product of PCR is purified with dialyse during 2h. </p> | ||
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| + | <h4> Competent cells </h4><p> | ||
To do sorbitol 0,20g/L and glycerol 10%. | To do sorbitol 0,20g/L and glycerol 10%. | ||
<br/>Pseudovibrio competent with wash sorbitol and glycerol in M9 medium. | <br/>Pseudovibrio competent with wash sorbitol and glycerol in M9 medium. | ||
Latest revision as of 20:20, 17 October 2014
Purification of pNK2 with Ori muted
The product of PCR is purified with dialyse during 2h.
Competent cells
To do sorbitol 0,20g/L and glycerol 10%.
Pseudovibrio competent with wash sorbitol and glycerol in M9 medium.
