Team:ETH Zurich/expresults/qs/tab-prhl

(Difference between revisions)

Revision as of 20:10, 17 October 2014

**Table 3** Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])
In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10³ nM. In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed. Severe crosstalk was detected in the case of 3OC6-HSL binding the RhlR regulator followed by induction of pRhl. The transition occurred at a concentration of the inducer molecule of 10³ nM but compared to the reference curve a lower (1000 AU) value of fluorescence was observed. The second case of crosstalk with the pRhl was detected with 3OC12-HSL binding to the corresponding LasR regulator followed by inducing the promoter pRhl. There the switching occurred at a concentration 1nM of 3OC12-HSL reaching a fluorescence of 750 AU. This is approximately 0.5 fold the value of the fluorescence shown by the reference curve in green.

@@ Line 1: / Line 1: @@
 {|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"
 |+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])
 |colspan="4" style='font-size:10pt';text-align:center|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM.
 In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed.