Team:Tuebingen/Notebook/Protocols/LB agar

From 2014.igem.org

(Difference between revisions)
 
Line 41: Line 41:
<ol>
<ol>
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   <li> Add the regents to 800ml H<sub>2</sub>O</li>
+
   <li> Add the reagents to 800 ml H<sub>2</sub>O</li>
   <li> Adjust pH to 7.5 with NaOH</li>
   <li> Adjust pH to 7.5 with NaOH</li>
Line 49: Line 49:
   <li> Melt agar into solution in the microwave</li>
   <li> Melt agar into solution in the microwave</li>
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   <li> Adjust volume to 1L with dH<sub>2</sub>O</li>
+
   <li> Adjust volume to 1 L with dH<sub>2</sub>O</li>
   <li> Sterilize by autoclaving</li>
   <li> Sterilize by autoclaving</li>

Latest revision as of 19:40, 17 October 2014


Protocols

LB agar

Reagents

10 g Bacto-tryptone
5 g yeast extract
10 g NaCl

 

Procedure

  1. Add the reagents to 800 ml H2O
  2. Adjust pH to 7.5 with NaOH
  3. Add 15g agar
  4. Melt agar into solution in the microwave
  5. Adjust volume to 1 L with dH2O
  6. Sterilize by autoclaving

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