Team:Groningen/Template/MODULE/Notebook/detection/week2

From 2014.igem.org

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4 August - 10 August
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August 4 - August 10
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Made fresh batch of competent cells.
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A new batch of cmpetent <i>E. coli</i> DH5α was prepared
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K592025 was ligated to B0015 with pSB2K3 plasmid backbone.
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A ligation was performed with the purple-blue chromoprotein amilCP BioBrick (<a href="http://parts.igem.org/Part:BBa_K592025">BBa_K592025</a>) together with the double terminator BioBrick (<a href="http://parts.igem.org/Part:BBa_ B0015">BBa_ B0015</a>) with the pSB2K3 vector
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Transformed into the <i>E. coli</i> DH5α and selected on Kanamycin plates.
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The ligation product was transformed to <i>E. coli</i> DH5α and plated on LB agar with kanamycin
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An overnight culture was grown, and the plasmid was isolated out of the culture in order obtain a high concentration of construct
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The construct was double digested, and checked on gel
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Transformed into the E.coli DH5α and selected on Kanamycin plates.
 
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Plasmids were isolated.
 
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Double digested to check the size of the insert
 

Latest revision as of 19:26, 17 October 2014

August 4 - August 10
 
A new batch of cmpetent E. coli DH5α was prepared
 
A ligation was performed with the purple-blue chromoprotein amilCP BioBrick (BBa_K592025) together with the double terminator BioBrick (BBa_ B0015) with the pSB2K3 vector
 
The ligation product was transformed to E. coli DH5α and plated on LB agar with kanamycin
 
An overnight culture was grown, and the plasmid was isolated out of the culture in order obtain a high concentration of construct
 
The construct was double digested, and checked on gel
 
 
 
 

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