Team:Evry/Interlab Study/09-16-2014

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A pre-culture was made with one colony for each construction to make a glycerol stock.<br>
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<i>PSB1C3+E0240+J23117</i> - <i>PSB1C3+E0240+J23119</i>:
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        <li> PCR colony of 6 colonies for each construction following protocol table 1 and 2.
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    <center>GEL 2: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23117 and PSB1C3+E0240+J23119 constructions.</center>
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A pre-culture was made with one colony for each construction to make a glycerol stock.<br>
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Latest revision as of 18:59, 17 October 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010



Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23100:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23101:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23102:
  • glycerol stock preparation from LB culture from the 14th September.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200.(18h30-1h30):
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23106:
  • glycerol stock preparation from LB culture from the 14th September.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200.(2h-9h):
IMAGE DU PLAN DE PLAQUE
PSB1C3+E0240+J23115 - PSB1C3+E0240+J23115:
  • PCR colony of 5 colonies for each construction following protocol table 1 and 2.
    IMAGE
    Symbol
    GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.

A pre-culture was made with one colony for each construction to make a glycerol stock.

PSB1C3+E0240+J23117 - PSB1C3+E0240+J23119:
  • PCR colony of 6 colonies for each construction following protocol table 1 and 2.
    IMAGE
    Symbol
    GEL 2: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23117 and PSB1C3+E0240+J23119 constructions.

A pre-culture was made with one colony for each construction to make a glycerol stock.


Sep 16

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