Team:Evry/Interlab Study/09-16-2014

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<h2>Other constructions of the Anderson library of constitutive promoters</h2>
<h2>Other constructions of the Anderson library of constitutive promoters</h2>
<br/>
<br/>
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<i>PSB1C3+E0240+J23100</i>: <br>
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Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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<br>
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<br>
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<i>PSB1C3+E0240+J23101</i>: <br>
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Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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<br>
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<br>
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<i>PSB1C3+E0240+J23102</i>:
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<ul>
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<li>glycerol stock preparation from LB culture from the 14th September.
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<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200.(18h30-1h30):
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</ul>
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<img src="https://static.igem.org/mediawiki/2014/6/62/J23114.26.09.png" alt="IMAGE DU PLAN DE PLAQUE" />
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<br>
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<br>
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<i>PSB1C3+E0240+J23106</i>:
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<ul>
 +
<li>glycerol stock preparation from LB culture from the 14th September.
 +
<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200.(2h-9h):
 +
</ul>
 +
<img src="https://static.igem.org/mediawiki/2014/2/2d/J23118.26.09_avec_dilution_40%C3%A05nM.png" alt="IMAGE DU PLAN DE PLAQUE" />
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<br>
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<i>PSB1C3+E0240+J23115</i> - <i>PSB1C3+E0240+J23115</i>:
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 +
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<ul>
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        <li> PCR colony of 5 colonies for each construction following protocol table 1 and 2.
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<div class="center">
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<div class="thumb tnone">
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  <div class="thumbinner" style="width:502px;">
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  <a href="https://static.igem.org/mediawiki/2014/4/45/115-116.jpg" class="image">
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    <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/4/45/115-116.jpg" width="450px;" class="thumbimage"/>
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  </a>
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  <div class="thumbcaption">
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    <div class="magnify">
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    <a href="https://static.igem.org/mediawiki/2014/4/45/115-116.jpg" class="internal" title="Enlarge">
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      <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/>
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    </a>
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    </div>
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    <center>GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.</center>
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  </div>
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  </div>
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</div>
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</div>
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      </ul>
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 +
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<br>
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A pre-culture was made with one colony for each construction to make a glycerol stock.<br>
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<br>
 +
 +
 +
 +
 +
 +
<i>PSB1C3+E0240+J23117</i> - <i>PSB1C3+E0240+J23119</i>:
 +
<br>
 +
 +
<ul>
 +
        <li> PCR colony of 6 colonies for each construction following protocol table 1 and 2.
 +
 +
<div class="center">
 +
<div class="thumb tnone">
 +
  <div class="thumbinner" style="width:502px;">
 +
  <a href="https://static.igem.org/mediawiki/2014/d/d5/117-119.jpg" class="image">
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    <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/d/d5/117-119.jpg" width="450px;" class="thumbimage"/>
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  </a>
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  <div class="thumbcaption">
 +
    <div class="magnify">
 +
    <a href="https://static.igem.org/mediawiki/2014/d/d5/117-119.jpg" class="internal" title="Enlarge">
 +
      <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/>
 +
    </a>
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    </div>
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    <center>GEL 2: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23117 and PSB1C3+E0240+J23119 constructions.</center>
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  </div>
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  </div>
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</div>
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</div>
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 +
      </ul>
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 +
<br>
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A pre-culture was made with one colony for each construction to make a glycerol stock.<br>
 +
<br>
 +
 +
 +
 +
<br>
<span class="cd-date">Sep 16</span>  
<span class="cd-date">Sep 16</span>  
</div>
</div>
</div>
</div>
</html>
</html>

Latest revision as of 18:59, 17 October 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010



Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23100:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23101:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23102:
  • glycerol stock preparation from LB culture from the 14th September.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200.(18h30-1h30):
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23106:
  • glycerol stock preparation from LB culture from the 14th September.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200.(2h-9h):
IMAGE DU PLAN DE PLAQUE
PSB1C3+E0240+J23115 - PSB1C3+E0240+J23115:
  • PCR colony of 5 colonies for each construction following protocol table 1 and 2.
    IMAGE
    Symbol
    GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.

A pre-culture was made with one colony for each construction to make a glycerol stock.

PSB1C3+E0240+J23117 - PSB1C3+E0240+J23119:
  • PCR colony of 6 colonies for each construction following protocol table 1 and 2.
    IMAGE
    Symbol
    GEL 2: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23117 and PSB1C3+E0240+J23119 constructions.

A pre-culture was made with one colony for each construction to make a glycerol stock.


Sep 16

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