Team:Evry/Interlab Study/09-18-2014

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<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2> <br>
<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2> <br>
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).<br>
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).<br>
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<img src="" alt="PLAN DE PLAQUE" />
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<img src="https://static.igem.org/mediawiki/2014/4/49/TECANnumber3.jpg" alt="PLAN DE PLAQUE" />
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<br>
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<h2>Other constructions of the Anderson library of constitutive promoters</h2>
<h2>Other constructions of the Anderson library of constitutive promoters</h2>
<br/>
<br/>
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<i>PSB1C3+E0240+J23105</i>:<br>
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<ul>
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<i>PSB1C3+E0240+J23101</i>: <br>  
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<li>glycerol stock preparation from LB culture from the 17th September.
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<li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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<li>Pre-culture of PSB1C3+E0240+J23105 in 3mL of LB+Cam (1:1000). Incubation 16h at 37°C.
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<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h43-23h43).<br>
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</ul>
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<img src="https://static.igem.org/mediawiki/2014/6/65/PlaqueLeft.png" alt="PLAN DE PLAQUE" />
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<br>
<br>
<br>
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<i>PSB1C3+E0240+J23102</i>: <br>
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<li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30). <br>
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<img src="https://static.igem.org/mediawiki/2014/6/62/J23114.26.09.png" alt="PLAN DE PLAQUE" />
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<br>
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<br>
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<i>PSB1C3+E0240+J23104</i>: <br>
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Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
 +
<br>
 +
<br>
 +
 +
<i>PSB1C3+E0240+J23105</i>: <br>
 +
glycerol stock preparation from LB culture of the 17th September.
 +
<br>
 +
<br>
<i>PSB1C3+E0240+J23107</i>: <br>
<i>PSB1C3+E0240+J23107</i>: <br>
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glycerol stock preparation from LB culture from the 17th September.  
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glycerol stock preparation from LB culture of the 17th September.  
 +
<br>
 +
<br>
 +
 
 +
 
 +
<i>PSB1C3+E0240+J23110</i>: <br>
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Pre-culture does'nt grow. The TECAN measure is reported.
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<br>
 +
<br>
 +
 
 +
<i>PSB1C3+E0240+J23115</i>: <br>
 +
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
 +
<br>
 +
<br>
 +
 
 +
<i>PSB1C3+E0240+J23116</i>: <br>
 +
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
 +
<br>
 +
<br>
 +
 
 +
<i>PSB1C3+E0240+J23117</i>: <br>
 +
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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<br>
 +
<br>
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<i>PSB1C3+E0240+J23114</i>:
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<ul>
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        <li> PCR colony of 5 colonies for each construction following protocol table 1 and 2.
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<div class="center">
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<div class="thumb tnone">
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  <div class="thumbinner" style="width:502px;">
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  <a href="https://static.igem.org/mediawiki/2014/7/75/114gel.jpg" class="image">
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    <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/7/75/114gel.jpg" width="450px;" class="thumbimage"/>
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  </a>
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  <div class="thumbcaption">
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    <div class="magnify">
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    <a href="https://static.igem.org/mediawiki/2014/7/75/114gel.jpg" class="internal" title="Enlarge">
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      <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/>
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    </a>
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    </div>
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    <center>GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23114 construction. </center>
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  </div>
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  </div>
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</div>
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</div>
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</ul>
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<br>
<br>
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A pre-culture was made with one colony to make a glycerol stock.<br>
<br>
<br>

Latest revision as of 18:59, 17 October 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010


Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).
PLAN DE PLAQUE

Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23101:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h43-23h43).
    PLAN DE PLAQUE

    PSB1C3+E0240+J23102:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30).
    PLAN DE PLAQUE

    PSB1C3+E0240+J23104:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

    PSB1C3+E0240+J23105:
    glycerol stock preparation from LB culture of the 17th September.

    PSB1C3+E0240+J23107:
    glycerol stock preparation from LB culture of the 17th September.

    PSB1C3+E0240+J23110:
    Pre-culture does'nt grow. The TECAN measure is reported.

    PSB1C3+E0240+J23115:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

    PSB1C3+E0240+J23116:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

    PSB1C3+E0240+J23117:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

    PSB1C3+E0240+J23114:
    • PCR colony of 5 colonies for each construction following protocol table 1 and 2.
      IMAGE
      Symbol
      GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23114 construction.

    A pre-culture was made with one colony to make a glycerol stock.

    Sep 18

    • Retrieved from "http://2014.igem.org/Team:Evry/Interlab_Study/09-18-2014"