Team:Evry/Interlab Study/09-11-2014

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<h2>Construction n°1: PSB1C3 with I20260</h2>
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<br/><h2>Construction n°2: PSB1C3 with J23101-E1010</h2>
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<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2>
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<h2>Constructions with other promoters</h2>
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<h2>Other constructions of the Anderson library of constitutive promoters</h2>
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PSB1C3+E0240+J23101 and PSB1C3+E0240+J23118:  
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<i>PSB1C3+E0240+J23101</i>, <i>PSB1C3+E0240+J23118</i>, <i>PSB1C3+E0240+J23105</i>, <i>PSB1C3+E0240+J23106</i> and <i>PSB1C3+E0240+J23107</i>:  
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         <li> Transformation plate from the 10th observation. There were 50 colonies for each constructions.
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         <li> Transformation plate from the 10th September observation. There were 50 colonies for each constructions.</ul>
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         <li> PCR colony of 8 colonies for each construction following protocol table 1 and 2.
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https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg
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<i>PSB1C3+E0240+J23101</i> and <i>PSB1C3+E0240+J23118</i>,<br>
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         <li> PCR colony of 3 colonies for each construction following protocol table 1 and 2.
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     <center>1% agarose gel. Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction.  Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction. </center>
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     <center>GEL 1: 1% agarose gel.<br>  Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction.<br> Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction. </center>
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        <li> PCR purification of sample 1 and 4 of the Gel 1 were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
         <li> Preparation of samples to sequencing. N° XX
         <li> Preparation of samples to sequencing. N° XX
         <li> Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C.
         <li> Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C.
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<br/> PSB1C3+E0240+J231xx:
 
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<i>PSB1C3+E0240+J23100</i>, <i>PSB1C3+E0240+J23102</i>, <i>PSB1C3+E0240+J23103</i>, <i>PSB1C3+E0240+J23104</i> and <i>PSB1C3+E0240+J23118</i>,<br>
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        <li> PCR colony of 1 colony for each construction following protocol table 1 and 2.
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    <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/f/f7/Gel_11.jpg" width="450px;" class="thumbimage"/>
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    <center>GEL 2: 1% agarose gel.<br>  Lane 1: PCR product of 1 colonies of the PSB1C3+E0240+J23100 construction.<br>  Lane 2: PCR product of 1 colonies of the PSB1C3+E0240+J23102 construction.<br> Lane 3: PCR product of 1 colonies of the PSB1C3+E0240+J23103 construction.<br> Lane 4: PCR product of 1 colonies of the PSB1C3+E0240+J23104 construction. </center>
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A pre-culture was made for each colony to make a glycerol stock.<br>
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<span class="cd-date">Sep 11</span>  
<span class="cd-date">Sep 11</span>  
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Latest revision as of 18:55, 17 October 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010



Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23101, PSB1C3+E0240+J23118, PSB1C3+E0240+J23105, PSB1C3+E0240+J23106 and PSB1C3+E0240+J23107:
  • Transformation plate from the 10th September observation. There were 50 colonies for each constructions.


PSB1C3+E0240+J23101 and PSB1C3+E0240+J23118,
  • PCR colony of 3 colonies for each construction following protocol table 1 and 2.
    IMAGE
    Symbol
    GEL 1: 1% agarose gel.
    Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction.
    Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction.
  • PCR purification of sample 1 and 4 of the Gel 1 were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
  • Preparation of samples to sequencing. N° XX
  • Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C.


PSB1C3+E0240+J23100, PSB1C3+E0240+J23102, PSB1C3+E0240+J23103, PSB1C3+E0240+J23104 and PSB1C3+E0240+J23118,
  • PCR colony of 1 colony for each construction following protocol table 1 and 2.
    IMAGE
    Symbol
    GEL 2: 1% agarose gel.
    Lane 1: PCR product of 1 colonies of the PSB1C3+E0240+J23100 construction.
    Lane 2: PCR product of 1 colonies of the PSB1C3+E0240+J23102 construction.
    Lane 3: PCR product of 1 colonies of the PSB1C3+E0240+J23103 construction.
    Lane 4: PCR product of 1 colonies of the PSB1C3+E0240+J23104 construction.

    A pre-culture was made for each colony to make a glycerol stock.

    Sep 11

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