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Team:Penn State/CodonOptimization
From 2014.igem.org
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Revision as of 18:23, 15 July 2014
WELCOME TO PENN STATE iGEM 2014!(Page under construction) |
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CODON OPTIMIZATION PROJECT | |||||||||||||
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Click HERE to return to the Projects page. Project Descriptiontell about project - give background, essentially write the abstract. (1-2 paragraphs) referencesiGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you. It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted. INFO TO INCLUDE:
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Click HERE to return to the Projects page.
Codon Optimization: Engineering a More Useful Gene at the Codon Level
Project Summary
Codons are groups of three nucleotides that specify a single amino acid, which is then added to a growing polypeptide chain during translation. Even though each codon spefifies only one amino acid, some amino acids are coded by multiple codons. It has been demonstrated that the genome of E.coli shows statistical preference for some of these degenerate codons over others, and it is hypothesized that these codons translate more efficiently than non preferred degenerate codons. We constructed synthetic reporter genes entirely from codons hypothesized to be fast or slow,and characterized them in E.coli, demonstrating that...
Why is this important?
Background
Our Objectives
1) Objective 1
2) Objective 2
3) Objective 3
4) Objective 4
5) Objective 5
Design Methods
The figure below shows the vector pFTV that was altered using inverse PCR.
Inverse PCR
Bold heading:
